Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OOT43_RS20150 Genome accession   NZ_CP110921
Coordinates   4287664..4289379 (+) Length   571 a.a.
NCBI ID   WP_266022503.1    Uniprot ID   -
Organism   Methylococcus mesophilus strain 16-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4282664..4294379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOT43_RS20135 (OOT43_20135) - 4282833..4284326 (+) 1494 WP_266022500.1 leucyl aminopeptidase -
  OOT43_RS20140 (OOT43_20140) - 4284323..4284754 (+) 432 WP_266022501.1 DNA polymerase III subunit chi -
  OOT43_RS20145 (OOT43_20145) - 4284849..4287614 (+) 2766 WP_266022502.1 valine--tRNA ligase -
  OOT43_RS20150 (OOT43_20150) pilB 4287664..4289379 (+) 1716 WP_266022503.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OOT43_RS20155 (OOT43_20155) pilC 4289384..4290598 (+) 1215 WP_266022504.1 type II secretion system F family protein Machinery gene
  OOT43_RS20160 (OOT43_20160) pilD 4290609..4291487 (+) 879 WP_266022505.1 A24 family peptidase Machinery gene
  OOT43_RS20165 (OOT43_20165) coaE 4291495..4292091 (+) 597 WP_266022506.1 dephospho-CoA kinase -
  OOT43_RS20170 (OOT43_20170) zapD 4292193..4292960 (+) 768 WP_266022507.1 cell division protein ZapD -
  OOT43_RS20175 (OOT43_20175) yacG 4292972..4293187 (+) 216 WP_266022508.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63191.85 Da        Isoelectric Point: 5.6701

>NTDB_id=756949 OOT43_RS20150 WP_266022503.1 4287664..4289379(+) (pilB) [Methylococcus mesophilus strain 16-5]
MAAVSGVQQLSGLAKSLVKAQLLSETDAQAYFEDARRKKTPFVSYLVSNKILDGLQIAQVAHQEFGLPLLDLDAVVFDIP
PTQWVSEKLIRRHHILPLLRRGNHLFVAASDPTNFLGLDEITFQTRLVTDCILVEENKLSRCIEAVLDAADTSLKQLLEE
DLDSLQITAGDDEGSHAEPEVSGIDDAPIVRFVNRLLLDAVRKGASDIHIEPYEKFLRIRFRHDGILHEVANPPAALGIK
ICARIKVMSRMDIAEHRIPQDGRIKMTLSRSRAIDFRVNTCPTLFGEKIVLRILDPGSAQIGVEMLGFEPEQRDNFLRAL
EKPYGMILVTGPTGSGKTVTLYTGLNLLNRVEANISTVEDPVEITVPGINQVNVNYKTGLTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIASSVLLIMAQRLARRLCPHCKREEKVPPEVLLA
SGFREEDIGNFTLYGPVGCEQCVKGYRGRVGIYQVMPISEEINRIILEGGNVMALTEQAHAEGIADLRESGLKKVRAGVT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=756949 OOT43_RS20150 WP_266022503.1 4287664..4289379(+) (pilB) [Methylococcus mesophilus strain 16-5]
ATGGCCGCGGTTTCCGGCGTCCAGCAACTGAGCGGCTTGGCGAAAAGCCTGGTCAAGGCCCAGCTGCTTTCGGAGACGGA
TGCCCAGGCTTACTTCGAGGATGCGCGCCGGAAGAAGACCCCTTTCGTCAGCTATCTCGTCTCCAACAAGATCCTCGACG
GTCTCCAGATCGCCCAGGTCGCCCATCAGGAGTTCGGGCTGCCGCTGCTCGACCTGGACGCGGTCGTCTTCGACATTCCC
CCGACCCAGTGGGTCAGCGAGAAGCTGATTCGCCGCCATCACATCCTGCCCCTGCTGCGGCGGGGCAACCATCTCTTCGT
CGCGGCCTCGGATCCGACCAATTTTCTGGGTCTGGACGAAATCACCTTCCAGACCCGTCTCGTCACCGATTGCATCCTGG
TCGAGGAGAACAAGCTTTCGCGCTGCATCGAAGCGGTCCTGGACGCGGCCGACACCAGCCTGAAGCAACTGCTCGAAGAG
GACCTGGACAGTCTCCAGATCACTGCCGGGGACGACGAAGGCTCGCACGCCGAACCGGAAGTTTCCGGCATCGACGATGC
GCCGATCGTGCGTTTCGTCAACCGGCTCTTGCTGGACGCGGTGCGAAAAGGGGCGTCGGACATCCATATCGAGCCCTACG
AAAAATTCCTGCGCATCCGGTTTCGGCACGACGGCATCCTGCACGAGGTCGCGAATCCTCCGGCGGCGCTGGGGATCAAG
ATATGCGCCCGCATCAAGGTGATGTCGCGCATGGACATCGCGGAACACCGGATTCCCCAGGACGGCCGCATCAAGATGAC
GCTGTCGCGCTCCCGCGCCATCGATTTCCGCGTCAATACCTGCCCGACGTTGTTCGGGGAAAAGATCGTGTTGCGTATCC
TCGATCCCGGTTCGGCCCAGATTGGCGTCGAGATGCTCGGATTCGAGCCGGAGCAGCGGGACAATTTCCTCCGCGCCCTG
GAAAAGCCCTATGGCATGATTCTCGTCACTGGGCCCACCGGCAGCGGCAAGACCGTCACTCTCTACACCGGCCTGAACCT
GCTCAATCGCGTCGAAGCCAACATCTCTACCGTGGAGGACCCGGTCGAAATCACGGTGCCCGGTATCAACCAGGTCAACG
TCAACTACAAGACCGGCCTCACGTTCGCCGAGGCGCTGCGCGCGTTTCTGCGCCAGGATCCCGACATCATCATGGTCGGC
GAGATCCGCGACCTGGAGACCGCCGAGATCGCCGTCAAGGCGGCCCAGACCGGCCATCTGGTACTTTCGACCCTGCACAC
CAACGATGCCCCTCAGACCTTGAACCGTCTGATGCAGATGGGGATCCCGGCGTTCAACATCGCCTCCTCCGTGCTCCTGA
TCATGGCGCAAAGGCTGGCCCGCAGGCTTTGTCCACATTGCAAACGGGAGGAGAAGGTTCCGCCCGAGGTTCTGCTCGCG
TCCGGCTTCCGGGAGGAAGACATCGGCAATTTCACGCTCTACGGACCGGTGGGCTGCGAGCAGTGCGTGAAGGGCTACAG
GGGGCGGGTCGGCATCTACCAGGTCATGCCGATTTCGGAGGAAATCAACCGCATCATCCTGGAGGGTGGGAACGTGATGG
CCCTGACCGAGCAGGCGCACGCGGAGGGCATCGCGGATTTGAGGGAGTCCGGCTTGAAGAAGGTGAGGGCGGGTGTCACC
AGCCTCGAGGAAATCGACCGTGTGACGAGAGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.87

100

0.573
  pilB Acinetobacter baylyi ADP1

53.287

100

0.539
  pilB Acinetobacter baumannii D1279779

52.007

100

0.522
  pilB Vibrio cholerae strain A1552

49.027

98.949

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

47.509

98.424

0.468
  pilF Neisseria gonorrhoeae MS11

47.34

98.774

0.468
  pilB Vibrio campbellii strain DS40M4

46.181

98.599

0.455
  pilF Thermus thermophilus HB27

38.137

99.65

0.38
  pilB/pilB1 Synechocystis sp. PCC 6803

38.6

97.548

0.377