Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   KI231_RS25065 Genome accession   NZ_AP021903
Coordinates   5589578..5590795 (+) Length   405 a.a.
NCBI ID   WP_213026616.1    Uniprot ID   -
Organism   Pseudomonas sp. Seg1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5584578..5595795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI231_RS25045 (PHLH6_49460) - 5585987..5586283 (+) 297 WP_213028839.1 DUF2845 domain-containing protein -
  KI231_RS25050 (PHLH6_49470) - 5586550..5586906 (-) 357 WP_213026614.1 BON domain-containing protein -
  KI231_RS25055 (PHLH6_49480) - 5587205..5587648 (-) 444 WP_103306537.1 pilin -
  KI231_RS25060 (PHLH6_49490) pilB 5587875..5589575 (+) 1701 WP_213026615.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI231_RS25065 (PHLH6_49500) pilC 5589578..5590795 (+) 1218 WP_213026616.1 type II secretion system F family protein Machinery gene
  KI231_RS25070 (PHLH6_49510) pilD 5590797..5591669 (+) 873 WP_213026617.1 A24 family peptidase Machinery gene
  KI231_RS25075 (PHLH6_49520) coaE 5591666..5592289 (+) 624 WP_213026618.1 dephospho-CoA kinase -
  KI231_RS25080 (PHLH6_49530) yacG 5592286..5592486 (+) 201 WP_213026619.1 DNA gyrase inhibitor YacG -
  KI231_RS25085 (PHLH6_49540) - 5592493..5592708 (-) 216 WP_007959584.1 hypothetical protein -
  KI231_RS25090 (PHLH6_49550) - 5592774..5593463 (-) 690 WP_213026620.1 energy-coupling factor ABC transporter permease -
  KI231_RS25095 (PHLH6_49560) - 5593460..5593927 (-) 468 WP_103306530.1 FAD/FMN-containing dehydrogenase -
  KI231_RS25100 (PHLH6_49570) - 5594058..5594684 (+) 627 WP_103306529.1 DUF1780 domain-containing protein -
  KI231_RS25105 (PHLH6_49580) - 5594681..5595208 (+) 528 WP_103306528.1 MOSC domain-containing protein -
  KI231_RS25110 (PHLH6_49590) - 5595260..5595433 (+) 174 WP_003228304.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44010.74 Da        Isoelectric Point: 10.0258

>NTDB_id=75689 KI231_RS25065 WP_213026616.1 5589578..5590795(+) (pilC) [Pseudomonas sp. Seg1]
MAVKAAKISVYAWEGTDKKGSKMTGELSGLNPALIKAQLRKQGINPGKVRKKSASILSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFANALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSEFMQQWWWAILGVLAAAIFGTR
HALKKSQALRDRKDTWLLNLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRTTGVFPNMAVQMTAIGEESGALDEMLDKVAGFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=75689 KI231_RS25065 WP_213026616.1 5589578..5590795(+) (pilC) [Pseudomonas sp. Seg1]
ATGGCGGTCAAGGCAGCGAAAATCAGTGTTTATGCCTGGGAAGGCACGGACAAGAAAGGCAGCAAAATGACCGGTGAGCT
GAGCGGGCTGAACCCGGCGCTGATCAAGGCGCAGTTGCGCAAACAGGGGATCAACCCTGGCAAGGTGCGCAAGAAATCCG
CCTCGATACTGAGCTTCGGTAAACGCATCAAGGCTCAGGACATCGCCCTGTTCACGCGGCAGATGGCGACCATGATGAAG
GCTGGCGTACCGTTGTTGCAGTCATTCGACATCATCGGCGAAGGTTTCGAAAACCCGGCGATGCGCAAACTGGTCGACGA
GGTGAAACAGGAAGTCGCTGCGGGTAACAGTTTCGCCAACGCCCTGCGCAAGAAACCGCAGTATTTCGACGAGTTGTACT
GCAACCTGGTGGATGCCGGCGAACAGTCCGGTGCCCTCGATACGTTGCTGGAGCGTGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCTACCGCCGTCGTGCTGGTCGCGGCGGTGGTGACCGGAAT
TTTGCTGGTGAAAGTGGTGCCACAGTTCCAGTCGGTGTTTTCCGGATTCGGTGCCGAGCTGCCGGCGTTCACCCTGATGG
TGATCAGCCTCTCCGAATTCATGCAGCAATGGTGGTGGGCGATTCTCGGTGTATTGGCGGCGGCGATTTTCGGCACCCGC
CACGCCCTGAAAAAGTCTCAGGCCTTGCGCGACCGCAAAGACACCTGGCTGCTGAATTTGCCGTTGGTGGGCACACTGAT
GTACAAGTCGGCCGTCGCCCGATTTGCTCGCACGCTGTCGACGACTTTTGCTGCTGGCGTGCCGCTGGTGGAAGCCCTCG
ACTCGGTGGCCGGGGCAACCGGCAACGTGGTGTTCAAGCGCGCGGTGTTGCGTATCCGCCAGGATGTTTCCACCGGTATG
CAGTTGAATTTTTCGATGCGCACCACCGGCGTCTTTCCCAATATGGCCGTGCAAATGACGGCGATCGGCGAAGAGTCTGG
CGCACTGGACGAGATGCTCGACAAGGTCGCCGGGTTTTACGAGGACGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TCATGGAGCCGTTCATCATGGTGGTCCTGGGGGTGATCGTCGGCGGTCTGGTAGTCGCCATGTACCTGCCGATTTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baylyi ADP1

59.804

100

0.602
  pilC Acinetobacter baumannii D1279779

59.069

100

0.595
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilC Vibrio cholerae strain A1552

43.687

97.778

0.427
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.133

100

0.412
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment