Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KI231_RS25060 Genome accession   NZ_AP021903
Coordinates   5587875..5589575 (+) Length   566 a.a.
NCBI ID   WP_213026615.1    Uniprot ID   -
Organism   Pseudomonas sp. Seg1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5582875..5594575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI231_RS25040 (PHLH6_49450) - 5583036..5585987 (+) 2952 WP_213026613.1 DUF748 domain-containing protein -
  KI231_RS25045 (PHLH6_49460) - 5585987..5586283 (+) 297 WP_213028839.1 DUF2845 domain-containing protein -
  KI231_RS25050 (PHLH6_49470) - 5586550..5586906 (-) 357 WP_213026614.1 BON domain-containing protein -
  KI231_RS25055 (PHLH6_49480) - 5587205..5587648 (-) 444 WP_103306537.1 pilin -
  KI231_RS25060 (PHLH6_49490) pilB 5587875..5589575 (+) 1701 WP_213026615.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI231_RS25065 (PHLH6_49500) pilC 5589578..5590795 (+) 1218 WP_213026616.1 type II secretion system F family protein Machinery gene
  KI231_RS25070 (PHLH6_49510) pilD 5590797..5591669 (+) 873 WP_213026617.1 A24 family peptidase Machinery gene
  KI231_RS25075 (PHLH6_49520) coaE 5591666..5592289 (+) 624 WP_213026618.1 dephospho-CoA kinase -
  KI231_RS25080 (PHLH6_49530) yacG 5592286..5592486 (+) 201 WP_213026619.1 DNA gyrase inhibitor YacG -
  KI231_RS25085 (PHLH6_49540) - 5592493..5592708 (-) 216 WP_007959584.1 hypothetical protein -
  KI231_RS25090 (PHLH6_49550) - 5592774..5593463 (-) 690 WP_213026620.1 energy-coupling factor ABC transporter permease -
  KI231_RS25095 (PHLH6_49560) - 5593460..5593927 (-) 468 WP_103306530.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62609.60 Da        Isoelectric Point: 5.9446

>NTDB_id=75688 KI231_RS25060 WP_213026615.1 5587875..5589575(+) (pilB) [Pseudomonas sp. Seg1]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
VDVESIDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKAIEIPRETLIKEGFPE
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTADLQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=75688 KI231_RS25060 WP_213026615.1 5587875..5589575(+) (pilB) [Pseudomonas sp. Seg1]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTTACGGAAAAAAGCGCCCAGCAAGC
CTGGCAACAGGCCCAGCGCAATCGCCTGTCGCTGGTCAGTTATCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTGG
CCGAGATCGCTTCGGAGCATTTCGGCATGGCCTTCATGGATCTTAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCCTGTGGCGCCGCGGCAACAAACTGTTCGTGGGCATCTCCGA
CCCGAGCAATCATCAGGCAATCAATGACATTCAGTTCAGCACCGGGCTGAGTACCGAAGCCATTCTGGTGGAGGACGACA
AACTCACCGATGCCATCGAAAAGTTCTTCGACACCCACGCCAGCGGCCTCGAAGACATGGCCGACGTCGACCTCGATGGC
GTTGATGTCGAATCCATCGACGACAGCAAGCAGGACGCCATCGGCGGACTCGACGCCGACGACGCGCCGGTGGTGCGCTT
CGTTCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCCTCCGACCTGCACTTCGAGCCCTACGAAAAGAACTACCGGG
TGCGCGTACGCACCGACGGCATTCTGCGCGAAGTGGCCAAGCCGCCGATTCAACTGGCCGGGAGAATCGCCGCACGGTTG
AAAGTCATGGCCAGCCTCGACATCTCGGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGAGCAA
GTCGATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGTG
CACAGATCGGCATCGATGCGCTCGGCTACGAGCCGGCGCAGAAAGACCTGTACATGGCCGCGCTCAAGCAGCCACAAGGC
ATGATTCTGGTCACCGGCCCCACCGGCTCAGGCAAGACCGTGTCGCTGTACACCGGGCTTAACATTCTCAATACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCTGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAACAGG
GCCTGGATTTCGCCCAGGCCCTGCGTTCGTTTCTGCGGCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGTGACCTC
GAAACCGCCGAGATCGCCATCAAGGCCGCGCAAACCGGCCACCTCGTACTCTCTACCCTGCATACCAACAGCGCAGCCGA
AACCCTGACCCGCCTGCACAACATGGGCATCCCCGGCTTCAATATCGCCACCTCGGTCAGCCTGATCATTGCCCAGCGAC
TGGCGCGCAAGCTGTGCAGCCATTGCAAGAAAGCCATCGAGATCCCCCGCGAAACCCTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCAGTTTCACGATCTACGAACCGGTCGGTTGCGATCACTGCAACGGCGGCTACAAGGGGCGCGTGGGGAT
TTATGAAGTGGTGAAAAACACAGCAGACCTGCAGCGACTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACATCCAGA
TGCGCCGCGACGGTTTCGATGACCTGCGCACTTCCGGCCTGCATAAAGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.558
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

49.734

99.47

0.495
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilF Thermus thermophilus HB27

36.738

98.587

0.362

Multiple sequence alignment