Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbB   Type   Machinery gene
Locus tag   OHI04_RS02085 Genome accession   NZ_CP110849
Coordinates   414951..415340 (+) Length   129 a.a.
NCBI ID   WP_010905314.1    Uniprot ID   S6EQX8
Organism   Lactococcus lactis strain VHProbi V60     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 414951..433720 414951..415340 within 0


Gene organization within MGE regions


Location: 414951..433720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHI04_RS02085 (OHI04_02085) ssbB 414951..415340 (+) 390 WP_010905314.1 single-stranded DNA-binding protein Machinery gene
  OHI04_RS02090 (OHI04_02090) groES 415460..415744 (+) 285 WP_003131589.1 co-chaperone GroES -
  OHI04_RS02095 (OHI04_02095) groL 415832..417460 (+) 1629 WP_003131585.1 chaperonin GroEL -
  OHI04_RS02100 (OHI04_02100) - 417506..418318 (-) 813 WP_039114610.1 MBL fold metallo-hydrolase -
  OHI04_RS02105 (OHI04_02105) - 418487..419929 (-) 1443 WP_052230896.1 sensor histidine kinase -
  OHI04_RS02110 (OHI04_02110) yycF 419922..420623 (-) 702 WP_003131580.1 response regulator YycF -
  OHI04_RS02115 (OHI04_02115) tmk 420801..421436 (+) 636 WP_012897230.1 dTMP kinase -
  OHI04_RS02120 (OHI04_02120) - 421569..422429 (+) 861 WP_003131573.1 DNA polymerase III subunit delta' -
  OHI04_RS02125 (OHI04_02125) ricT 422481..423263 (+) 783 WP_039114615.1 PSP1 domain-containing protein -
  OHI04_RS02130 (OHI04_02130) yabA 423256..423582 (+) 327 WP_003131570.1 DNA replication initiation control protein YabA -
  OHI04_RS02135 (OHI04_02135) rsmI 423582..424457 (+) 876 WP_039114617.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  OHI04_RS02140 (OHI04_02140) - 424534..424725 (-) 192 WP_012897234.1 hypothetical protein -
  OHI04_RS02145 (OHI04_02145) - 425754..426086 (-) 333 WP_039114618.1 hypothetical protein -
  OHI04_RS02150 (OHI04_02150) - 426086..426397 (-) 312 WP_039114619.1 hypothetical protein -
  OHI04_RS02155 (OHI04_02155) - 426646..427218 (-) 573 WP_155276098.1 hypothetical protein -
  OHI04_RS02160 (OHI04_02160) - 427232..427546 (-) 315 WP_039114623.1 hypothetical protein -
  OHI04_RS02165 (OHI04_02165) - 427666..427815 (-) 150 WP_153951467.1 hypothetical protein -
  OHI04_RS02170 (OHI04_02170) - 427826..428719 (-) 894 WP_039114625.1 ATP-binding protein -
  OHI04_RS02175 (OHI04_02175) - 428731..429573 (-) 843 WP_052230897.1 phage replisome organizer protein -
  OHI04_RS02180 (OHI04_02180) - 429582..429908 (-) 327 WP_023163763.1 HTH domain-containing protein -
  OHI04_RS02185 (OHI04_02185) - 429895..430158 (-) 264 WP_039114627.1 hypothetical protein -
  OHI04_RS02190 (OHI04_02190) - 430172..430441 (-) 270 WP_039114629.1 hypothetical protein -
  OHI04_RS02195 (OHI04_02195) - 430438..430956 (-) 519 WP_052230898.1 hypothetical protein -
  OHI04_RS02200 (OHI04_02200) - 430961..431155 (-) 195 WP_023163769.1 DUF1655 domain-containing protein -
  OHI04_RS02205 (OHI04_02205) - 431283..431546 (-) 264 WP_039114631.1 hypothetical protein -
  OHI04_RS02210 (OHI04_02210) - 431584..431778 (-) 195 WP_039114633.1 transcriptional regulator -
  OHI04_RS02215 (OHI04_02215) - 431925..432461 (+) 537 WP_080756578.1 helix-turn-helix domain-containing protein -
  OHI04_RS02220 (OHI04_02220) - 432521..433720 (+) 1200 WP_039114635.1 tyrosine-type recombinase/integrase -

Sequence

Protein


Download         Length: 129 a.a.        Molecular weight: 14672.73 Da        Isoelectric Point: 8.3705

>NTDB_id=756602 OHI04_RS02085 WP_010905314.1 414951..415340(+) (ssbB) [Lactococcus lactis strain VHProbi V60]
MNKTMLIGRLTSAPEISKTTNDKSYVRVTLAVNRRFKNEKGEREADFISIIIWGKSAETLVSYAKKGSLISIEGEIRTRN
YTDKQNQKHYVTEILGLSYDLLESRATIALRESAIKTEETLLDAEELPF

Nucleotide


Download         Length: 390 bp        

>NTDB_id=756602 OHI04_RS02085 WP_010905314.1 414951..415340(+) (ssbB) [Lactococcus lactis strain VHProbi V60]
ATGAATAAAACCATGTTAATTGGACGCTTAACCAGTGCGCCAGAAATTTCAAAAACAACAAATGATAAATCATATGTCCG
TGTGACCTTAGCAGTTAATCGCCGTTTCAAAAATGAAAAAGGAGAACGAGAGGCAGATTTTATTTCAATTATTATTTGGG
GAAAATCTGCAGAAACTTTAGTTTCTTACGCAAAAAAAGGAAGTCTTATTTCAATTGAAGGAGAAATTAGAACTAGAAAT
TATACGGATAAGCAAAATCAAAAACATTATGTCACAGAAATCTTAGGACTGAGTTATGATTTACTTGAAAGTAGGGCAAC
AATCGCTTTACGAGAAAGCGCTATAAAGACTGAAGAAACCTTACTTGATGCGGAAGAACTCCCTTTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(1-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6EQX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Lactococcus lactis subsp. cremoris KW2

90.698

100

0.907
  ssbB Streptococcus sobrinus strain NIDR 6715-7

60.769

100

0.612
  ssbB/cilA Streptococcus pneumoniae TIGR4

56.923

100

0.574
  ssbB/cilA Streptococcus pneumoniae Rx1

56.154

100

0.566
  ssbB/cilA Streptococcus pneumoniae D39

56.154

100

0.566
  ssbB/cilA Streptococcus pneumoniae R6

56.154

100

0.566
  ssbB/cilA Streptococcus mitis NCTC 12261

56.154

100

0.566
  ssbB/cilA Streptococcus mitis SK321

56.154

100

0.566
  ssbA Streptococcus mutans UA159

55.385

100

0.558
  ssb Latilactobacillus sakei subsp. sakei 23K

43.75

86.822

0.38
  ssbA Bacillus subtilis subsp. subtilis str. 168

44.762

81.395

0.364