Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FQS62_RS17640 Genome accession   NZ_CP109812
Coordinates   3810912..3812171 (+) Length   419 a.a.
NCBI ID   WP_099589893.1    Uniprot ID   -
Organism   Stenotrophomonas sp. SBJS02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3796924..3809992 3810912..3812171 flank 920


Gene organization within MGE regions


Location: 3796924..3812171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FQS62_RS17575 (FQS62_017575) - 3796924..3797598 (+) 675 WP_141097651.1 hypothetical protein -
  FQS62_RS17580 (FQS62_017580) - 3797592..3798605 (+) 1014 WP_049447473.1 hypothetical protein -
  FQS62_RS17585 (FQS62_017585) - 3798599..3799615 (+) 1017 WP_208042994.1 TraB/GumN family protein -
  FQS62_RS17590 (FQS62_017590) - 3799934..3800203 (+) 270 WP_049447475.1 hypothetical protein -
  FQS62_RS17595 (FQS62_017595) - 3800255..3802012 (+) 1758 WP_208042995.1 ABC transporter transmembrane domain-containing protein -
  FQS62_RS17600 (FQS62_017600) sucD 3802130..3803005 (-) 876 WP_017356169.1 succinate--CoA ligase subunit alpha -
  FQS62_RS17605 (FQS62_017605) sucC 3803026..3804195 (-) 1170 WP_208042996.1 ADP-forming succinate--CoA ligase subunit beta -
  FQS62_RS17610 (FQS62_017610) - 3804601..3806214 (+) 1614 WP_049447477.1 ATP-binding protein -
  FQS62_RS17615 (FQS62_017615) pilR 3806275..3807648 (+) 1374 WP_049447478.1 sigma-54 dependent transcriptional regulator Regulator
  FQS62_RS17620 (FQS62_017620) pilB 3807754..3809482 (-) 1729 Protein_3445 type IV-A pilus assembly ATPase PilB -
  FQS62_RS22305 - 3809576..3809992 (-) 417 WP_208043038.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FQS62_RS17635 (FQS62_017635) pilA2 3810098..3810550 (-) 453 WP_208043037.1 pilin Machinery gene
  FQS62_RS17640 (FQS62_017640) pilC 3810912..3812171 (+) 1260 WP_099589893.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45882.38 Da        Isoelectric Point: 10.1923

>NTDB_id=749533 FQS62_RS17640 WP_099589893.1 3810912..3812171(+) (pilC) [Stenotrophomonas sp. SBJS02]
MSVSRSAIKKEPVARATTELLPFVWEGTDKRGVKMKGEQLAKNANLLRAELRKQGINPGQVKAKPKPLFGAAGSAVKPKD
IAFFSRQMATMMKSGVPIVSSLEIIAGGHKNPRMKKLVETIRTDIEGGSSLHEAISRHPVQFDELYRNLVRAGEGAGVLE
TVLETIATYKENMEALKGKIKKAMFYPAMIVLVAFVVSAILLMFVVPQFEEVFKSFGAELPAFTQMIVAASRFMLSWWWL
LLIVLVGSSVGFFMAYKRSPKMQHTFDRLVLKVPVIGQIMNNSAIARFARTTAVTFKAGVPLVEALGIVAGATGNKVYEE
GVLRMRDDVSVGYPVNMAMKQLNLFPHMVIQMTGIGEEAGALDTMLFKVAEYYEQEVNNAVDALSSLLEPMIMVFIGTIV
GGIVIGMYLPIFKLGAVVG

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=749533 FQS62_RS17640 WP_099589893.1 3810912..3812171(+) (pilC) [Stenotrophomonas sp. SBJS02]
ATGTCTGTCAGTCGCAGTGCGATCAAGAAAGAGCCCGTGGCCCGCGCGACCACGGAACTGCTGCCGTTTGTCTGGGAGGG
GACCGACAAGCGGGGCGTGAAGATGAAAGGCGAGCAGTTGGCAAAGAACGCCAATCTGCTGCGGGCGGAGCTACGTAAGC
AGGGGATCAACCCCGGCCAGGTCAAAGCCAAGCCGAAGCCGCTGTTCGGCGCAGCGGGAAGTGCCGTCAAGCCGAAAGAC
ATCGCCTTCTTCAGCCGGCAGATGGCCACGATGATGAAGTCCGGCGTCCCGATCGTTTCGTCACTGGAGATCATTGCCGG
GGGCCACAAGAATCCCCGGATGAAGAAGCTGGTCGAGACGATCCGTACCGACATCGAAGGCGGCTCTTCGCTGCACGAGG
CGATCAGCCGTCACCCGGTGCAGTTCGACGAGCTCTACCGCAACCTTGTACGTGCGGGTGAAGGCGCAGGTGTGCTCGAA
ACTGTGCTGGAAACCATTGCGACCTACAAAGAGAACATGGAAGCCCTGAAGGGCAAGATCAAAAAGGCCATGTTCTACCC
GGCCATGATCGTCCTTGTGGCCTTTGTAGTGTCAGCCATCCTGCTGATGTTTGTGGTTCCGCAGTTTGAAGAGGTTTTCA
AGAGCTTCGGCGCCGAGTTGCCTGCGTTTACTCAGATGATCGTGGCTGCCTCGCGATTCATGCTCTCCTGGTGGTGGTTG
CTGCTGATTGTCCTTGTGGGCAGTTCTGTAGGCTTCTTCATGGCCTACAAGCGCTCTCCCAAGATGCAGCACACCTTTGA
CCGTTTGGTGCTCAAAGTGCCGGTCATAGGGCAGATCATGAACAACAGCGCGATTGCTCGTTTCGCCCGCACCACGGCGG
TGACCTTCAAGGCCGGTGTGCCACTGGTGGAGGCCCTGGGCATCGTGGCTGGCGCCACCGGCAACAAGGTCTACGAAGAA
GGCGTGCTGCGGATGCGCGACGACGTGTCGGTCGGCTATCCGGTCAACATGGCCATGAAGCAGCTGAACCTGTTCCCGCA
CATGGTCATCCAGATGACCGGCATCGGCGAAGAGGCCGGCGCGCTGGATACCATGCTTTTCAAGGTGGCCGAGTATTATG
AGCAGGAGGTCAACAATGCCGTGGACGCCTTGAGCAGCCTGCTGGAGCCGATGATCATGGTGTTCATTGGTACCATTGTC
GGTGGCATCGTGATCGGCATGTACCTCCCCATCTTCAAACTCGGCGCCGTCGTCGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)

Type II secretion system (T2SS), protein F (T2SSF)

(288-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.788

94.511

0.508
  pilC Legionella pneumophila strain ERS1305867

51.629

95.227

0.492
  pilC Acinetobacter baylyi ADP1

50

97.375

0.487
  pilC Acinetobacter baumannii D1279779

49.502

95.943

0.475
  pilG Neisseria meningitidis 44/76-A

42.356

95.227

0.403
  pilG Neisseria gonorrhoeae MS11

42.569

94.749

0.403
  pilC Vibrio cholerae strain A1552

40.741

96.659

0.394
  pilC Vibrio campbellii strain DS40M4

39.651

95.704

0.379
  pilC Thermus thermophilus HB27

38.025

96.659

0.368