Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACRAD_RS13125 Genome accession   NZ_AP019740
Coordinates   2772847..2775027 (-) Length   726 a.a.
NCBI ID   WP_005024212.1    Uniprot ID   -
Organism   Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2767847..2780027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACRAD_RS13110 (ACRAD_25620) - 2770284..2771147 (-) 864 WP_005024218.1 hypothetical protein -
  ACRAD_RS13115 (ACRAD_25630) aroB 2771155..2772240 (-) 1086 WP_005024217.1 3-dehydroquinate synthase -
  ACRAD_RS13120 (ACRAD_25640) aroK 2772259..2772810 (-) 552 WP_005024215.1 shikimate kinase AroK -
  ACRAD_RS13125 (ACRAD_25650) pilQ 2772847..2775027 (-) 2181 WP_005024212.1 type IV pilus secretin PilQ Machinery gene
  ACRAD_RS13130 (ACRAD_25660) pilP 2775045..2775572 (-) 528 WP_005024210.1 pilus assembly protein PilP Machinery gene
  ACRAD_RS13135 (ACRAD_25670) comO 2775569..2776279 (-) 711 WP_005024207.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACRAD_RS13140 (ACRAD_25680) pilN 2776276..2776935 (-) 660 WP_005018044.1 PilN domain-containing protein Machinery gene
  ACRAD_RS13145 (ACRAD_25690) comM 2776935..2777993 (-) 1059 WP_005024205.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 726 a.a.        Molecular weight: 78765.46 Da        Isoelectric Point: 5.4971

>NTDB_id=73759 ACRAD_RS13125 WP_005024212.1 2772847..2775027(-) (pilQ) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
MKKSFQEFLMGDGNSMIHLYRQFAMGAVAVAMMQVASAAVNITNIVPMQIPGQGTEIRVMFNGLPPQPQAYQLEQPSRLI
LDFDQAKQNLKQPSIPVATNEASAIHVASDEQRSRIIVSLKDAGAFTTRVEGNTFILKINPVTGAPTVLPQPVAVAQQPA
QGISNIGFQRGSQGEGQVLIDFLNPNTPADVQQQGSKVIVRMLGNKIPSHLIRRLNMNDFATPVSTVDAYNEGGNGIITI
QSTGSYEYMAYQTENRLTVSLKRPEEKSTVKTRSAPNYSGKKVSLDFQDIEVRRVLQLLADFTGINLVAADSVQGNITLR
LKDVPWDQALDIILKTKNLDKRRNGDVIWIAPVAELIKAEEEEAKAIAQSTRLMPLQTEYIQLNYAKAVDIEKLITQGRN
SGNGSANGASNNTEALGDSVGSLLSPRGTISIDPRTNTLIINDTAQKLDQIRRMIDLLDVAVKQVMVEARIVRASTDFTK
EMGVKWGILSQGITRNNSLLVGGSETTLWNLRDPDDEGKYTIERPQNLNVDLGVTAPGASRIAFGLISLSDFMLDLELSA
LQADGYGEVISTPKVLTADKQQARVASGTQIPYQSSEGGGANAVSTTEFVDATLSLNVTPSITPDGKIQMLLNITSDTPG
NPTPTGQIAINKNEINTNVLVNNGETVVLGGIFEQETRNTQTKVPVLGDLPGVGRLFRRDAKSDNKRELLIFVTPRIVND
SATGNH

Nucleotide


Download         Length: 2181 bp        

>NTDB_id=73759 ACRAD_RS13125 WP_005024212.1 2772847..2775027(-) (pilQ) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
ATGAAAAAGAGTTTTCAAGAATTTTTGATGGGGGATGGCAACAGCATGATTCATTTATATCGTCAATTTGCGATGGGAGC
AGTTGCTGTAGCAATGATGCAGGTAGCAAGTGCAGCGGTCAATATTACCAATATTGTACCTATGCAGATTCCGGGACAAG
GGACAGAAATTCGAGTCATGTTTAATGGATTGCCACCCCAGCCCCAGGCTTATCAGCTTGAACAGCCTTCGCGACTGATC
TTAGACTTTGATCAGGCCAAGCAAAATCTTAAACAGCCTAGTATTCCAGTTGCTACCAATGAAGCAAGCGCAATTCATGT
GGCCTCTGATGAACAACGTTCCCGAATTATTGTAAGTTTAAAAGATGCAGGTGCCTTTACTACCCGTGTAGAAGGCAATA
CTTTTATTTTAAAGATTAATCCGGTAACAGGCGCGCCTACAGTATTGCCTCAGCCAGTTGCTGTAGCACAGCAGCCTGCT
CAGGGTATCTCGAACATTGGTTTCCAGCGTGGAAGTCAGGGCGAGGGACAGGTTTTAATTGATTTTCTGAACCCGAATAC
TCCTGCTGATGTACAGCAGCAGGGAAGTAAAGTCATAGTCAGGATGCTGGGAAATAAAATCCCTTCCCATCTAATCCGCC
GCTTGAATATGAATGATTTTGCCACGCCGGTCTCGACTGTTGATGCCTATAATGAAGGCGGTAATGGTATTATTACTATT
CAATCAACTGGCAGTTATGAATATATGGCGTACCAGACTGAGAACCGCTTAACAGTCAGTTTAAAGCGCCCGGAAGAAAA
GAGTACGGTCAAAACCCGTTCTGCACCTAATTATAGCGGTAAAAAGGTTTCACTCGACTTTCAGGATATTGAAGTTCGTC
GGGTATTACAGTTGTTAGCAGATTTCACTGGTATCAACCTGGTTGCAGCCGACTCTGTTCAGGGAAATATTACGTTGCGC
CTGAAAGATGTACCATGGGACCAGGCTTTAGACATTATTTTAAAAACCAAAAACCTGGATAAACGTCGTAATGGTGATGT
AATCTGGATTGCTCCGGTTGCAGAGCTGATTAAAGCAGAAGAGGAAGAAGCTAAGGCGATTGCGCAAAGTACCAGACTTA
TGCCTTTACAAACTGAATATATCCAGCTGAATTATGCAAAAGCTGTCGATATCGAAAAACTGATTACCCAAGGCAGAAAT
AGTGGTAATGGTAGTGCTAACGGTGCTTCAAACAATACGGAAGCACTGGGTGATAGCGTGGGAAGCCTGCTAAGTCCAAG
AGGAACCATTTCAATAGACCCACGTACCAATACCTTAATCATTAATGATACGGCTCAGAAACTTGACCAGATCCGCCGTA
TGATTGATCTTCTGGATGTAGCGGTAAAACAGGTGATGGTTGAGGCCCGTATTGTCAGGGCTAGTACTGACTTCACCAAA
GAAATGGGCGTGAAGTGGGGAATTCTTTCACAGGGTATTACCCGTAATAACAGCCTGCTGGTCGGCGGCAGTGAAACCAC
CTTATGGAATCTGCGTGATCCTGATGATGAAGGCAAATACACCATAGAGCGTCCACAAAACCTGAATGTGGATTTAGGCG
TTACAGCTCCGGGTGCAAGCCGTATTGCCTTCGGTTTAATTAGTCTTTCTGATTTTATGCTGGATCTCGAATTGTCAGCC
TTGCAGGCTGATGGTTATGGTGAAGTCATTTCTACGCCGAAAGTACTAACAGCTGACAAACAGCAGGCCCGAGTTGCTTC
TGGTACCCAAATTCCCTACCAGTCTTCAGAGGGCGGTGGCGCCAATGCAGTCTCTACCACTGAATTTGTGGATGCTACTT
TAAGTCTGAATGTAACACCAAGTATTACCCCTGATGGTAAAATCCAGATGTTGCTGAACATTACCAGCGATACTCCGGGG
AACCCGACACCAACCGGACAGATTGCGATTAACAAGAATGAAATTAATACTAATGTGCTGGTTAACAATGGTGAAACTGT
AGTCCTTGGCGGTATTTTTGAACAGGAAACCAGGAATACGCAAACGAAAGTACCTGTTCTCGGAGACCTGCCGGGTGTAG
GCCGTTTATTCCGCCGTGATGCTAAAAGTGATAACAAGCGTGAGTTACTGATTTTTGTCACACCACGAATTGTTAATGAC
AGTGCGACTGGAAATCATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-352)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-464)

AMIN domain (AMIN)

(55-136)

Bacterial type II and III secretion system protein (Secretin)

(560-718)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

73.416

100

0.734
  pilQ Acinetobacter baumannii strain A118

73.278

100

0.733
  comQ Acinetobacter baylyi ADP1

73.072

98.209

0.718
  pilQ Pseudomonas aeruginosa PAK

39.669

100

0.397
  pilQ Legionella pneumophila strain Lp02

38.384

95.455

0.366
  pilQ Legionella pneumophila strain ERS1305867

38.273

95.73

0.366

Multiple sequence alignment