Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACRAD_RS13140 Genome accession   NZ_AP019740
Coordinates   2776276..2776935 (-) Length   219 a.a.
NCBI ID   WP_005018044.1    Uniprot ID   A0A062C2K4
Organism   Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2771276..2781935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACRAD_RS13120 (ACRAD_25640) aroK 2772259..2772810 (-) 552 WP_005024215.1 shikimate kinase AroK -
  ACRAD_RS13125 (ACRAD_25650) pilQ 2772847..2775027 (-) 2181 WP_005024212.1 type IV pilus secretin PilQ Machinery gene
  ACRAD_RS13130 (ACRAD_25660) pilP 2775045..2775572 (-) 528 WP_005024210.1 pilus assembly protein PilP Machinery gene
  ACRAD_RS13135 (ACRAD_25670) comO 2775569..2776279 (-) 711 WP_005024207.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACRAD_RS13140 (ACRAD_25680) pilN 2776276..2776935 (-) 660 WP_005018044.1 PilN domain-containing protein Machinery gene
  ACRAD_RS13145 (ACRAD_25690) comM 2776935..2777993 (-) 1059 WP_005024205.1 pilus assembly protein PilM Machinery gene
  ACRAD_RS13150 (ACRAD_25700) ponA 2778155..2780701 (+) 2547 WP_005024203.1 penicillin-binding protein PBP1a -
  ACRAD_RS13155 (ACRAD_25710) - 2780748..2781554 (-) 807 WP_005024201.1 putative RNA methyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24229.61 Da        Isoelectric Point: 4.6921

>NTDB_id=73762 ACRAD_RS13140 WP_005018044.1 2776276..2776935(-) (pilN) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
MTTINLLPWRDELREQRKKQFIIICIGVALLGLAGVVLTWMYYDRKLDDQEQANQLIISTNQNLDIQLKSLDGLQEQRDA
IIERMNLIQGLQGKRPVTVRLVDELARVIPANMYLTKFARTGDKFTLEGKAESPNTVAELLRGLEASPWYRNAFMNSFLA
AEEKKEQTPTSLIPRPEENYGSFVVTVDAGEIAADSPPSADPNAPTNTAATGPAAGAAQ

Nucleotide


Download         Length: 660 bp        

>NTDB_id=73762 ACRAD_RS13140 WP_005018044.1 2776276..2776935(-) (pilN) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
ATGACAACAATTAACCTGCTCCCTTGGCGTGATGAGCTAAGGGAACAACGGAAAAAGCAATTTATTATTATTTGTATTGG
GGTGGCATTACTAGGTTTAGCCGGAGTGGTACTGACTTGGATGTACTATGACCGCAAGCTGGATGATCAGGAACAAGCTA
ATCAACTGATTATCAGTACTAACCAGAACCTGGATATACAGCTTAAATCTCTAGATGGCCTGCAAGAACAGCGTGATGCC
ATTATTGAGCGCATGAACCTGATTCAGGGCTTACAGGGTAAACGTCCGGTTACTGTTCGTTTGGTGGATGAGCTGGCACG
TGTAATCCCAGCCAATATGTATCTGACCAAATTTGCACGGACTGGTGATAAATTCACTCTCGAAGGGAAGGCAGAAAGCC
CGAACACTGTAGCTGAGCTTTTGCGTGGTCTAGAAGCTTCACCTTGGTATCGTAATGCCTTTATGAACTCATTCCTTGCC
GCGGAAGAAAAGAAAGAGCAGACCCCAACTTCGCTGATACCTCGACCAGAAGAAAACTATGGCAGTTTTGTTGTAACTGT
AGATGCAGGTGAGATTGCTGCTGATAGTCCGCCATCGGCTGATCCCAATGCACCAACCAATACAGCAGCAACGGGTCCTG
CAGCGGGAGCAGCACAATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A062C2K4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

71.364

100

0.717
  comN Acinetobacter baylyi ADP1

68.545

97.26

0.667

Multiple sequence alignment