Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   FNL60_RS01225 Genome accession   NZ_AP019720
Coordinates   237384..238325 (+) Length   313 a.a.
NCBI ID   WP_002264247.1    Uniprot ID   -
Organism   Streptococcus mutans strain NBRC 13955     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 232384..243325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNL60_RS01215 (SM3g_02070) rpoC 233114..236743 (+) 3630 WP_002263433.1 DNA-directed RNA polymerase subunit beta' -
  FNL60_RS01220 (SM3g_02080) - 236871..237254 (+) 384 WP_002264248.1 DUF1033 family protein -
  FNL60_RS01225 (SM3g_02090) comYA 237384..238325 (+) 942 WP_002264247.1 competence type IV pilus ATPase ComGA Machinery gene
  FNL60_RS01230 (SM3g_02100) comYB 238261..239292 (+) 1032 WP_255262721.1 competence type IV pilus assembly protein ComGB Machinery gene
  FNL60_RS01235 (SM3g_02110) comYC 239292..239606 (+) 315 WP_002263437.1 competence type IV pilus major pilin ComGC Machinery gene
  FNL60_RS01240 (SM3g_02120) comYD 239593..239997 (+) 405 WP_002264246.1 competence type IV pilus minor pilin ComGD Machinery gene
  FNL60_RS01245 (SM3g_02130) comYE 239969..240262 (+) 294 WP_002263439.1 competence type IV pilus minor pilin ComGE Machinery gene
  FNL60_RS01250 (SM3g_02140) comYF 240249..240683 (+) 435 WP_002264245.1 competence type IV pilus minor pilin ComGF Machinery gene
  FNL60_RS01255 (SM3g_02150) comYG 240661..241050 (+) 390 WP_002263441.1 competence type IV pilus minor pilin ComGG Machinery gene
  FNL60_RS01260 (SM3g_02160) comYH 241105..242058 (+) 954 WP_002264244.1 class I SAM-dependent methyltransferase Machinery gene
  FNL60_RS01265 (SM3g_02170) - 242117..243316 (+) 1200 WP_002263443.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35667.03 Da        Isoelectric Point: 8.5765

>NTDB_id=73497 FNL60_RS01225 WP_002264247.1 237384..238325(+) (comYA) [Streptococcus mutans strain NBRC 13955]
MVQKLGRKIIAEAVQNKAQDIYIIPKKESYELYMRIGNERRFIDVYDHHFMANLISHFKFVAGMNVGEKRRSQLGSCDYS
YDEGKVISLRLSSVGDYRGYESLVIRLLFSGRHDLKFWFNSMDLILNEIKGRGLYLFSGPVGSGKTSLMYQLVKLRFGTK
QVITIEDPVEIKQDNMLQLQLNESIDMTYDNLIKLSLRHRPDILIIGEIRDKETARAVIRASLTGAMVFSTIHAKSIPGV
YARMLELGTSSEELQNALRGIVYQRLIGGGGIIDFAKGDYQNHSAKKWNKQIYDLFTAGYITEGEAQAEEIED

Nucleotide


Download         Length: 942 bp        

>NTDB_id=73497 FNL60_RS01225 WP_002264247.1 237384..238325(+) (comYA) [Streptococcus mutans strain NBRC 13955]
ATGGTTCAAAAATTAGGTAGAAAAATCATTGCCGAAGCTGTTCAAAATAAAGCACAAGATATCTATATTATTCCTAAAAA
AGAATCTTATGAGCTTTATATGAGAATAGGGAATGAAAGGAGGTTCATTGATGTTTATGACCATCATTTCATGGCTAATC
TTATTAGTCACTTTAAATTTGTTGCAGGAATGAATGTTGGTGAGAAAAGACGAAGTCAGCTGGGCTCTTGTGATTATTCT
TATGATGAAGGGAAAGTTATTTCATTAAGGTTATCAAGTGTAGGAGATTATCGTGGTTATGAAAGTTTGGTTATTCGCTT
ACTTTTTTCTGGACGTCACGATTTAAAATTCTGGTTTAACAGTATGGATTTGATTTTAAATGAAATCAAGGGGCGAGGGC
TTTATCTTTTTTCAGGTCCAGTTGGAAGTGGCAAAACAAGTTTGATGTATCAATTGGTAAAACTAAGATTTGGGACCAAA
CAGGTAATTACCATTGAAGATCCTGTGGAAATTAAGCAAGATAATATGTTACAACTGCAGCTTAATGAAAGCATTGATAT
GACTTATGATAATTTAATTAAATTATCTCTGAGGCATCGTCCGGATATTTTAATTATTGGAGAAATTCGAGATAAAGAAA
CCGCCAGAGCTGTTATTAGAGCAAGTCTTACAGGGGCAATGGTATTCTCAACTATCCATGCTAAAAGCATTCCAGGTGTT
TATGCTAGGATGTTAGAATTGGGTACTAGTTCAGAAGAATTACAAAATGCTTTGCGTGGTATTGTTTATCAACGTTTAAT
AGGGGGAGGAGGGATTATTGACTTTGCCAAAGGGGATTATCAAAACCACTCAGCAAAAAAGTGGAACAAACAAATTTATG
ACCTTTTTACAGCAGGATATATCACTGAAGGAGAAGCACAAGCAGAAGAAATTGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

99.681

100

0.997
  comYA Streptococcus mutans UA140

99.361

100

0.994
  comYA Streptococcus gordonii str. Challis substr. CH1

67.093

100

0.671
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

66.026

99.681

0.658
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

65.916

99.361

0.655
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

65.916

99.361

0.655
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.916

99.361

0.655
  comGA/cglA/cilD Streptococcus pneumoniae D39

65.916

99.361

0.655
  comGA/cglA/cilD Streptococcus pneumoniae R6

65.916

99.361

0.655
  comGA Lactococcus lactis subsp. cremoris KW2

52.412

99.361

0.521

Multiple sequence alignment