Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N5O83_RS03645 Genome accession   NZ_CP104579
Coordinates   774586..775803 (-) Length   405 a.a.
NCBI ID   WP_055984076.1    Uniprot ID   A0AA42TU78
Organism   Pseudomonas oleovorans strain GD04132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 769586..780803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O83_RS03605 (N5O83_03610) - 769657..769830 (-) 174 WP_169971266.1 DUF3094 family protein -
  N5O83_RS03610 (N5O83_03615) - 770055..770609 (-) 555 WP_279534406.1 MOSC domain-containing protein -
  N5O83_RS03615 (N5O83_03620) - 770606..771235 (-) 630 WP_004425012.1 DUF1780 domain-containing protein -
  N5O83_RS03620 (N5O83_03625) - 771446..772135 (+) 690 WP_279534407.1 energy-coupling factor ABC transporter permease -
  N5O83_RS03625 (N5O83_03630) - 772242..772457 (+) 216 WP_247723116.1 hypothetical protein -
  N5O83_RS03630 (N5O83_03635) yacG 772621..772815 (-) 195 WP_017678823.1 DNA gyrase inhibitor YacG -
  N5O83_RS03635 (N5O83_03640) coaE 772812..773420 (-) 609 WP_004425004.1 dephospho-CoA kinase -
  N5O83_RS03640 (N5O83_03645) pilD 773716..774585 (-) 870 WP_279534408.1 A24 family peptidase Machinery gene
  N5O83_RS03645 (N5O83_03650) pilC 774586..775803 (-) 1218 WP_055984076.1 type II secretion system F family protein Machinery gene
  N5O83_RS03650 (N5O83_03655) pilB 775806..777509 (-) 1704 WP_279534409.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5O83_RS03655 (N5O83_03660) pilE 777847..778386 (+) 540 WP_279534410.1 pilin Machinery gene
  N5O83_RS03660 (N5O83_03665) tfpZ 778550..779284 (+) 735 WP_279534411.1 TfpX/TfpZ family type IV pilin accessory protein -
  N5O83_RS03665 (N5O83_03670) - 779453..779775 (-) 323 Protein_726 nicotinate-nucleotide diphosphorylase (carboxylating) -
  N5O83_RS03670 (N5O83_03675) - 779848..780246 (+) 399 WP_279534412.1 nucleotidyltransferase domain-containing protein -
  N5O83_RS03675 (N5O83_03680) - 780236..780658 (+) 423 WP_278620745.1 DUF86 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43949.71 Da        Isoelectric Point: 9.8564

>NTDB_id=730619 N5O83_RS03645 WP_055984076.1 774586..775803(-) (pilC) [Pseudomonas oleovorans strain GD04132]
MAGKALKTSVFTWEGKNKSGAVVKGELSGQNPSLVKAQLRKQGINPTKVRKKGVSLFSAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIISEGFDNPNMRKLVDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVESGEQAGALENLLDRIATYKEKT
EALKAKIKKAMNYPIAVVVVAIIVSAILLIKVVPQFEEVFANFGAELPAFTLMVIGISQFLQEWWFIFLIAIIAAAFAFK
EAMKRSQKLRDSVDRGVLKLPIVGDILYKSAVARFARTLSTTFAAGVPLVDALDSVSGATGNVVFKNATNKIKADVSTGM
QLNFSMRTTGTFPSMAVQMTAIGEESGSLDEMLDKVASFYEAEVDNMVDGLTSLMEPIIMSVLGVLIGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=730619 N5O83_RS03645 WP_055984076.1 774586..775803(-) (pilC) [Pseudomonas oleovorans strain GD04132]
ATGGCGGGAAAAGCTCTAAAAACCAGTGTGTTCACCTGGGAAGGCAAGAACAAAAGCGGTGCCGTCGTCAAGGGGGAGCT
CAGCGGGCAGAACCCGTCCTTGGTCAAGGCGCAACTGCGCAAACAGGGGATCAATCCGACCAAAGTACGCAAAAAGGGGG
TGTCGCTCTTCAGCGCAGGCAAGAAGATCAAACCCATGGATATCGCCCTGTTTACCCGGCAGATGGCGACCATGATGAAA
GCTGGCGTGCCACTGCTGCAGTCCTTCGACATCATCTCAGAGGGCTTCGACAACCCCAACATGCGCAAGCTGGTCGACGA
GGTGAAGCAGGAGGTGGCTGCTGGTAACAGTTTTGCCGCCTCGCTGCGCAAGAAACCACAGTACTTCGATGAACTGTATT
GCAACCTGGTTGAGTCTGGCGAACAGGCCGGCGCGCTGGAGAATCTGCTGGACCGAATCGCCACCTACAAAGAAAAGACC
GAAGCGCTGAAGGCCAAGATCAAGAAGGCGATGAACTATCCAATCGCTGTTGTGGTGGTCGCCATCATCGTCTCAGCCAT
TTTGCTCATTAAAGTTGTACCGCAATTCGAGGAAGTTTTCGCCAACTTCGGCGCTGAACTACCTGCCTTTACGCTGATGG
TAATAGGCATATCGCAATTTCTTCAGGAGTGGTGGTTCATTTTTCTGATTGCAATCATCGCCGCTGCCTTTGCATTCAAA
GAAGCGATGAAGCGCTCGCAGAAACTGCGCGACTCGGTTGACCGTGGCGTACTGAAACTGCCAATCGTCGGCGACATTCT
TTACAAATCTGCGGTAGCCCGCTTCGCACGCACACTATCAACGACTTTTGCCGCTGGCGTGCCACTGGTCGATGCGCTGG
ATTCGGTCTCCGGCGCCACCGGTAATGTGGTGTTTAAGAATGCGACCAACAAGATCAAAGCAGATGTATCTACCGGCATG
CAGCTTAACTTCTCCATGCGCACCACCGGCACCTTCCCCTCCATGGCCGTGCAGATGACCGCTATTGGCGAAGAGTCTGG
CTCGCTGGATGAGATGCTCGATAAGGTAGCGAGCTTCTATGAAGCAGAAGTGGACAACATGGTTGACGGCCTGACCAGCC
TGATGGAACCCATCATCATGTCCGTACTGGGCGTACTGATCGGCGGCCTAATCATCGCCATGTACCTACCTATCTTCCAG
CTGGGCGCCGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.469

100

0.825
  pilC Acinetobacter baylyi ADP1

60.553

98.272

0.595
  pilC Acinetobacter baumannii D1279779

59.653

99.753

0.595
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

45.771

99.259

0.454
  pilG Neisseria meningitidis 44/76-A

45.771

99.259

0.454
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.404

97.778

0.395