NTDB

Overview

Name	pilE	Type	Machinery gene
Locus tag	N5O83_RS03655	Genome accession	NZ_CP104579
Coordinates	777847..778386 (+)	Length	179 a.a.
NCBI ID	WP_279534410.1	Uniprot ID	A0AA42TYW7
Organism	Pseudomonas oleovorans strain GD04132
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 772847..783386

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5O83_RS03640 (N5O83_03645)	pilD	773716..774585 (-)	870	WP_279534408.1	A24 family peptidase	Machinery gene
N5O83_RS03645 (N5O83_03650)	pilC	774586..775803 (-)	1218	WP_055984076.1	type II secretion system F family protein	Machinery gene
N5O83_RS03650 (N5O83_03655)	pilB	775806..777509 (-)	1704	WP_279534409.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N5O83_RS03655 (N5O83_03660)	pilE	777847..778386 (+)	540	WP_279534410.1	pilin	Machinery gene
N5O83_RS03660 (N5O83_03665)	tfpZ	778550..779284 (+)	735	WP_279534411.1	TfpX/TfpZ family type IV pilin accessory protein	-
N5O83_RS03665 (N5O83_03670)	-	779453..779775 (-)	323	Protein_726	nicotinate-nucleotide diphosphorylase (carboxylating)	-
N5O83_RS03670 (N5O83_03675)	-	779848..780246 (+)	399	WP_279534412.1	nucleotidyltransferase domain-containing protein	-
N5O83_RS03675 (N5O83_03680)	-	780236..780658 (+)	423	WP_278620745.1	DUF86 domain-containing protein	-
N5O83_RS03680 (N5O83_03685)	-	780912..781301 (-)	390	WP_004424977.1	type II toxin-antitoxin system VapC family toxin	-
N5O83_RS03685 (N5O83_03690)	-	781298..781555 (-)	258	WP_004424974.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
N5O83_RS03690 (N5O83_03695)	-	782946..783317 (-)	372	Protein_731	nicotinate-nucleotide diphosphorylase (carboxylating)	-

Sequence

Protein

Download Length: 179 a.a. Molecular weight: 18128.78 Da Isoelectric Point: 7.7775

                                >NTDB_id=730621 N5O83_RS03655 WP_279534410.1 777847..778386(+) (pilE) [Pseudomonas oleovorans strain GD04132]

MKAQMQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRTRITEGLNLAQPAKLAIATEGSASAADLARVATTWNAQANNL

GATSKYVTSVLITGASGLITITYDATDVGVAAAANTLTLTPWIRDSAAGRSLSAAQAAGVTGSIDWGCASATNVTATANG

ITPLAAGTLLAKYAPAECR

Nucleotide

Download Length: 540 bp

                                >NTDB_id=730621 N5O83_RS03655 WP_279534410.1 777847..778386(+) (pilE) [Pseudomonas oleovorans strain GD04132]

ATGAAAGCTCAAATGCAAAAGGGTTTTACCCTGATCGAATTGATGATCGTGGTTGCGATTATTGGTATTCTGGCGGCCGT

TGCGCTGCCGGCTTATCAGGACTATACCGTACGCACCCGGATCACCGAAGGATTGAATCTGGCGCAACCTGCAAAATTAG

CTATTGCTACAGAAGGTTCTGCTTCAGCCGCTGATCTGGCTCGAGTGGCTACCACTTGGAATGCTCAGGCGAACAACCTT

GGCGCGACTTCCAAGTATGTTACTTCTGTGTTGATTACTGGCGCATCTGGTCTGATTACCATTACCTATGATGCGACCGA

CGTAGGTGTCGCTGCTGCTGCGAATACCTTGACTTTGACTCCTTGGATTCGTGACTCCGCGGCTGGCCGGTCTCTCTCTG

CTGCTCAAGCCGCTGGTGTTACCGGTTCTATCGATTGGGGTTGTGCGTCTGCCACGAATGTTACCGCTACAGCGAACGGG

ATTACTCCTCTTGCCGCAGGTACTTTGCTAGCTAAGTACGCTCCGGCTGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	45	100	0.503
pilA	Ralstonia pseudosolanacearum GMI1000	43.407	100	0.441
pilA2	Legionella pneumophila str. Paris	44.253	97.207	0.43
pilA2	Legionella pneumophila strain ERS1305867	44.253	97.207	0.43
pilA	Vibrio parahaemolyticus RIMD 2210633	45.638	83.24	0.38
pilA/pilAI	Pseudomonas stutzeri DSM 10701	37.222	100	0.374

Detailed information NTG0730621