Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N5O83_RS03650 Genome accession   NZ_CP104579
Coordinates   775806..777509 (-) Length   567 a.a.
NCBI ID   WP_279534409.1    Uniprot ID   A0AA42TZZ4
Organism   Pseudomonas oleovorans strain GD04132     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 770806..782509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O83_RS03620 (N5O83_03625) - 771446..772135 (+) 690 WP_279534407.1 energy-coupling factor ABC transporter permease -
  N5O83_RS03625 (N5O83_03630) - 772242..772457 (+) 216 WP_247723116.1 hypothetical protein -
  N5O83_RS03630 (N5O83_03635) yacG 772621..772815 (-) 195 WP_017678823.1 DNA gyrase inhibitor YacG -
  N5O83_RS03635 (N5O83_03640) coaE 772812..773420 (-) 609 WP_004425004.1 dephospho-CoA kinase -
  N5O83_RS03640 (N5O83_03645) pilD 773716..774585 (-) 870 WP_279534408.1 A24 family peptidase Machinery gene
  N5O83_RS03645 (N5O83_03650) pilC 774586..775803 (-) 1218 WP_055984076.1 type II secretion system F family protein Machinery gene
  N5O83_RS03650 (N5O83_03655) pilB 775806..777509 (-) 1704 WP_279534409.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5O83_RS03655 (N5O83_03660) pilE 777847..778386 (+) 540 WP_279534410.1 pilin Machinery gene
  N5O83_RS03660 (N5O83_03665) tfpZ 778550..779284 (+) 735 WP_279534411.1 TfpX/TfpZ family type IV pilin accessory protein -
  N5O83_RS03665 (N5O83_03670) - 779453..779775 (-) 323 Protein_726 nicotinate-nucleotide diphosphorylase (carboxylating) -
  N5O83_RS03670 (N5O83_03675) - 779848..780246 (+) 399 WP_279534412.1 nucleotidyltransferase domain-containing protein -
  N5O83_RS03675 (N5O83_03680) - 780236..780658 (+) 423 WP_278620745.1 DUF86 domain-containing protein -
  N5O83_RS03680 (N5O83_03685) - 780912..781301 (-) 390 WP_004424977.1 type II toxin-antitoxin system VapC family toxin -
  N5O83_RS03685 (N5O83_03690) - 781298..781555 (-) 258 WP_004424974.1 type II toxin-antitoxin system prevent-host-death family antitoxin -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62492.52 Da        Isoelectric Point: 5.3460

>NTDB_id=730620 N5O83_RS03650 WP_279534409.1 775806..777509(-) (pilB) [Pseudomonas oleovorans strain GD04132]
MNESISLSGLARQLAQAELMDEKSAQQAQLQAQRNKLPLVTYLVQNKLVKSRVLAELAAEQFGVAFFDLNVIDKEGQPRD
LVSEKLVRQHRVLPLWRRGNKLFVALSDPTNHQAVTDVQFSTGLTTEAILVEDDKLGDAIDKFFDSANSGMDELADVDLD
GLDVEAVDDEKSVGEGGADADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGILHEVARPPIQLAPRISAR
LKVMAALDISERRKPQDGRIKMKISKSKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYMSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNNCKKEVNVPRETLLAEGFP
EDKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPNLQRIIMEEGNSIDIAAQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=730620 N5O83_RS03650 WP_279534409.1 775806..777509(-) (pilB) [Pseudomonas oleovorans strain GD04132]
ATGAACGAGAGCATTTCCCTTTCAGGCCTGGCCAGGCAGTTGGCACAGGCCGAACTGATGGACGAAAAATCCGCCCAGCA
GGCCCAACTGCAGGCCCAGCGCAACAAGCTGCCGCTGGTGACCTATCTGGTGCAGAACAAGCTGGTGAAAAGCCGAGTCC
TGGCCGAGTTGGCCGCCGAACAGTTCGGCGTGGCTTTTTTCGATCTGAATGTAATCGACAAAGAAGGCCAGCCACGGGAT
CTGGTCAGCGAGAAACTGGTTCGCCAGCATCGTGTGCTGCCGCTCTGGCGCAGAGGCAACAAACTGTTCGTGGCGCTCTC
CGACCCGACCAATCACCAGGCCGTCACCGATGTGCAGTTCAGCACCGGCCTGACCACAGAAGCAATTTTGGTCGAGGACG
ACAAACTCGGCGATGCCATCGACAAGTTCTTCGACTCGGCCAATAGCGGCATGGACGAGCTGGCAGACGTTGATCTGGAT
GGGCTGGATGTCGAGGCGGTTGATGATGAGAAGTCGGTCGGCGAAGGTGGCGCCGACGCTGACGATGCGCCGGTAGTGCG
CTTCGTCAACAAGATGCTGCTGGATGCCATCAAAGGCGGCTCTTCCGACCTGCACTTCGAGCCTTACGAAAAAGCCTATC
GGGTACGCTTTCGTACCGACGGCATCCTCCATGAGGTCGCTCGCCCGCCCATCCAGCTGGCGCCGCGAATTTCCGCCCGT
CTCAAGGTGATGGCCGCGCTAGATATCTCTGAGCGCCGCAAGCCGCAGGATGGCCGGATCAAGATGAAGATTTCCAAGAG
CAAGGCCATCGACTTTCGCGTCAACACCCTGCCCACACTCTGGGGCGAAAAGATCGTGATGCGGATTCTCGACCCCACAA
GCGCGCAGATGGGCATCGACGCACTGGGCTACGAGGAGGAGCAGAAAGAGCTGTATATGAGCGCCCTCAAACAGCCTCAG
GGCATGATTCTGGTCACCGGCCCCACTGGCTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATCCTCAACACGCC
TGATGTGAATATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAGGGTATCAACCAGGTCAACGTCAACCCCAAGC
AGGGCATGGACTTCACCCAGGCGCTGCGCGCCTTTCTGCGTCAGGACCCGGACATCATCATGGTCGGTGAGATCCGCGAC
CTGGACACAGCCTCTATTGCCGTCAAGGCCGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACCCGCCTGCGCAATATGGGCGTGCCTTCCTTCAATATCGCCACCTCGGTCAACCTGATCATCGCCCAGC
GTCTGGCGCGCAAGCTGTGCAACAACTGCAAGAAGGAAGTGAACGTACCGCGCGAGACGCTGCTGGCCGAAGGTTTCCCC
GAAGACAAGATCGGCACATTCAAAATCTACGGCCCGGTCGGTTGCGATAACTGCAAGGGCGGTTACAAAGGGCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCCAACCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATTGCCGCGC
AGATGCGTAAAGACGGCTTTAATGACCTACGCACCTCCGCTTTGCTCAAGGCCATGCAAGGGGTCACCAGCCTTGAGGAA
GTCAACCGCGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.844

100

0.593
  pilB Acinetobacter baylyi ADP1

58.761

99.647

0.586
  pilB Legionella pneumophila strain ERS1305867

54.657

100

0.548
  pilB Vibrio cholerae strain A1552

49.383

100

0.494
  pilF Neisseria gonorrhoeae MS11

49.293

99.824

0.492
  pilB Vibrio campbellii strain DS40M4

47.544

100

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

50.755

93.474

0.474
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.113

93.651

0.376