Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N4269_RS02300 Genome accession   NZ_CP104356
Coordinates   478174..479862 (-) Length   562 a.a.
NCBI ID   WP_134987328.1    Uniprot ID   -
Organism   Vibrio cholerae strain PNUSAV001141     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473174..484862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4269_RS02275 (N4269_02275) yacG 474224..474421 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  N4269_RS02280 (N4269_02280) zapD 474595..475335 (-) 741 WP_000207194.1 cell division protein ZapD -
  N4269_RS02285 (N4269_02285) coaE 475367..475975 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  N4269_RS02290 (N4269_02290) pilD 475972..476847 (-) 876 WP_001960918.1 A24 family peptidase Machinery gene
  N4269_RS02295 (N4269_02295) pilC 476905..478131 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  N4269_RS02300 (N4269_02300) pilB 478174..479862 (-) 1689 WP_134987328.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N4269_RS02305 (N4269_02305) - 479863..480324 (-) 462 WP_162906052.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N4269_RS02310 (N4269_02310) nadC 480569..481459 (-) 891 WP_134987327.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N4269_RS02315 (N4269_02315) ampD 481755..482300 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N4269_RS02320 (N4269_02320) - 482344..483732 (-) 1389 WP_001959226.1 IS4-like element ISVa18 family transposase -
  N4269_RS02325 (N4269_02325) fldB 483871..484389 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62002.08 Da        Isoelectric Point: 6.2588

>NTDB_id=729044 N4269_RS02300 WP_134987328.1 478174..479862(-) (pilB) [Vibrio cholerae strain PNUSAV001141]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHPQELTGQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=729044 N4269_RS02300 WP_134987328.1 478174..479862(-) (pilB) [Vibrio cholerae strain PNUSAV001141]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCGACGCAAGAACAAGCGGTGGTTACCCAGGT
TAGCGCTTCGGGAACTTCAGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAAGAACTGACCGGGCAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATCACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTACTGCTGCTGGCGGTCTCTGACCCAACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTACTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCTGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGTTTACCGCAAGATGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGAATGATT
TTGATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTGTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCATTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAAATCGCGGTAAAAGCGGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCATACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTTGCGTCATCACTCAGTTTAATCATCGCCCAGCGTCTTGCGC
GCCGCTTGTGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCATTTCAGCCCAATGAA
ATCTTGTATGAAGCGAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAGCGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TACTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.288

100

0.993
  pilB Vibrio parahaemolyticus RIMD 2210633

73.665

100

0.737
  pilB Vibrio campbellii strain DS40M4

73.488

100

0.735
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baumannii D1279779

50

92.527

0.463
  pilB Acinetobacter baylyi ADP1

50.884

90.569

0.461
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37

100

0.395
  pilF Thermus thermophilus HB27

39.668

96.441

0.383
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB Haemophilus influenzae Rd KW20

39.279

93.772

0.368