Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N4269_RS02295 Genome accession   NZ_CP104356
Coordinates   476905..478131 (-) Length   408 a.a.
NCBI ID   WP_000648511.1    Uniprot ID   Q9X4G9
Organism   Vibrio cholerae strain PNUSAV001141     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471905..483131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4269_RS02275 (N4269_02275) yacG 474224..474421 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  N4269_RS02280 (N4269_02280) zapD 474595..475335 (-) 741 WP_000207194.1 cell division protein ZapD -
  N4269_RS02285 (N4269_02285) coaE 475367..475975 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  N4269_RS02290 (N4269_02290) pilD 475972..476847 (-) 876 WP_001960918.1 A24 family peptidase Machinery gene
  N4269_RS02295 (N4269_02295) pilC 476905..478131 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  N4269_RS02300 (N4269_02300) pilB 478174..479862 (-) 1689 WP_134987328.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N4269_RS02305 (N4269_02305) - 479863..480324 (-) 462 WP_162906052.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N4269_RS02310 (N4269_02310) nadC 480569..481459 (-) 891 WP_134987327.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N4269_RS02315 (N4269_02315) ampD 481755..482300 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45134.43 Da        Isoelectric Point: 10.3424

>NTDB_id=729043 N4269_RS02295 WP_000648511.1 476905..478131(-) (pilC) [Vibrio cholerae strain PNUSAV001141]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=729043 N4269_RS02295 WP_000648511.1 476905..478131(-) (pilC) [Vibrio cholerae strain PNUSAV001141]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAACTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTGTCTCTTTTGGCACGCCTAACCCATCGCGTGAAAAGTAAAGATATTACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCGATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAAGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATCAAAGCGCTCATCTACCCCAGCATGGTGGTGTTGGTCGCGCTCGGAGTTTC
CTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCAGAACTGCCTTGGTTTACGC
AGCAAGTACTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCCTTCCAGATCCGTTTAAAAACCAGTCGCTTGGGGCTGAAATTTCCGATTATTGGCAA
TGTACTCGCCAAAGCGTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACGGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTATATTGCCATGCGCAATACTGAAGCTTTTCCGGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCAGGGCAATTAGATGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACAGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTGTTTCTTGGGACGGTCGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9X4G9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

100

100

1
  pilC Vibrio campbellii strain DS40M4

73.75

98.039

0.723
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402