Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   N4269_RS02290 Genome accession   NZ_CP104356
Coordinates   475972..476847 (-) Length   291 a.a.
NCBI ID   WP_001960918.1    Uniprot ID   -
Organism   Vibrio cholerae strain PNUSAV001141     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470972..481847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4269_RS02270 (N4269_02270) parC 471890..474175 (+) 2286 WP_000102510.1 DNA topoisomerase IV subunit A -
  N4269_RS02275 (N4269_02275) yacG 474224..474421 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  N4269_RS02280 (N4269_02280) zapD 474595..475335 (-) 741 WP_000207194.1 cell division protein ZapD -
  N4269_RS02285 (N4269_02285) coaE 475367..475975 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  N4269_RS02290 (N4269_02290) pilD 475972..476847 (-) 876 WP_001960918.1 A24 family peptidase Machinery gene
  N4269_RS02295 (N4269_02295) pilC 476905..478131 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  N4269_RS02300 (N4269_02300) pilB 478174..479862 (-) 1689 WP_134987328.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N4269_RS02305 (N4269_02305) - 479863..480324 (-) 462 WP_162906052.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N4269_RS02310 (N4269_02310) nadC 480569..481459 (-) 891 WP_134987327.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32463.72 Da        Isoelectric Point: 7.1618

>NTDB_id=729042 N4269_RS02290 WP_001960918.1 475972..476847(-) (pilD) [Vibrio cholerae strain PNUSAV001141]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGTWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=729042 N4269_RS02290 WP_001960918.1 475972..476847(-) (pilD) [Vibrio cholerae strain PNUSAV001141]
ATGGAACTGTTTTACTTCTACCCTTGGCTTTTTCCGGTGCTCGCCACCCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTACCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTGGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCTACTCACTTTCCGTTCGGGGTGTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTGCGGCGACCTTCATCGACTTCGATACCCTGTTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCGGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCTATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCACTCGGCACTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTATGGGGCGATAAGGTGATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(135-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

99.656

100

0.997
  pilD Vibrio campbellii strain DS40M4

75

98.969

0.742
  pilD Acinetobacter nosocomialis M2

53.309

93.471

0.498
  pilD Acinetobacter baumannii D1279779

52.941

93.471

0.495
  pilD Neisseria gonorrhoeae MS11

44.444

98.969

0.44