Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N4J17_RS16320 Genome accession   NZ_CP104311
Coordinates   3382977..3384191 (-) Length   404 a.a.
NCBI ID   WP_198322880.1    Uniprot ID   -
Organism   Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3377977..3389191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4J17_RS16285 (N4J17_16295) - 3378002..3378664 (+) 663 WP_198322873.1 HAD family hydrolase -
  N4J17_RS16290 (N4J17_16300) - 3378673..3378903 (-) 231 WP_198322874.1 sulfurtransferase TusA family protein -
  N4J17_RS16295 (N4J17_16305) cysG 3378951..3380375 (+) 1425 WP_198322875.1 siroheme synthase CysG -
  N4J17_RS16300 (N4J17_16310) - 3380385..3380600 (-) 216 WP_198322876.1 DNA gyrase inhibitor YacG -
  N4J17_RS16305 (N4J17_16315) zapD 3380613..3381326 (-) 714 WP_338457616.1 cell division protein ZapD -
  N4J17_RS16310 (N4J17_16320) coaE 3381481..3382077 (-) 597 WP_198322878.1 dephospho-CoA kinase -
  N4J17_RS16315 (N4J17_16325) pilD 3382085..3382930 (-) 846 WP_277458359.1 A24 family peptidase Machinery gene
  N4J17_RS16320 (N4J17_16330) pilC 3382977..3384191 (-) 1215 WP_198322880.1 type II secretion system F family protein Machinery gene
  N4J17_RS16325 (N4J17_16335) pilB 3384196..3385911 (-) 1716 WP_198322881.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N4J17_RS16330 (N4J17_16340) - 3385961..3388726 (-) 2766 WP_198322882.1 valine--tRNA ligase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 44070.84 Da        Isoelectric Point: 9.6120

>NTDB_id=728581 N4J17_RS16320 WP_198322880.1 3382977..3384191(-) (pilC) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
MPNQETALFVWEGLDRNGSRTRGEISARSEITARAELRRQGIRVVKIKKKPKPLFGGPHKKITPKHIAVFSRQLATMLSA
GVPLVQAFDIVGRGHDNPAMQDLLMSIKADVEAGTTLADALSKHPVYFDELFCNLVRAGEQAGVLETLLHKVADYKEKTE
FLKGKIKKALAYPIAVVVVAVIVTSILLIFVVPTFEDLFKSFGADLPAFTQMVINLSRWLRDWWYVVFGSLGVATAAFVK
ARQRSPAFNHLLDRLALGLPVVGAILHKAAIARFARTLSTMSAAGVPLVEALQSVAGATGNIIYGDVVMRMREEVSIGQQ
LQMSMRQANLFPNMVVQMVAIGEESGALDSMLSKVADFYEEEVDNAVDSLSSLLEPLIMVVLGILVGGLVIAMYLPIFKL
GSVV

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=728581 N4J17_RS16320 WP_198322880.1 3382977..3384191(-) (pilC) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
ATGCCGAATCAGGAAACCGCTTTGTTCGTTTGGGAGGGCCTGGACAGGAACGGCAGTCGGACCCGGGGGGAAATCAGCGC
CCGGTCGGAGATCACGGCGCGGGCGGAGCTCAGACGTCAGGGCATCCGCGTCGTCAAGATCAAGAAGAAGCCCAAGCCCT
TGTTCGGCGGTCCACACAAGAAGATCACACCCAAGCACATCGCCGTGTTCAGCCGCCAGTTGGCGACCATGCTGTCGGCC
GGGGTTCCCCTAGTCCAGGCATTCGACATCGTGGGCCGCGGCCATGACAATCCGGCTATGCAGGATCTGCTGATGAGCAT
CAAGGCCGACGTGGAAGCCGGCACGACCCTGGCGGATGCCCTGTCGAAGCACCCGGTCTATTTCGACGAGCTTTTCTGTA
ATCTGGTTCGGGCCGGCGAACAGGCCGGTGTGCTGGAAACCTTGTTGCACAAGGTGGCCGACTACAAGGAAAAAACCGAG
TTCCTCAAGGGCAAGATCAAAAAGGCTTTGGCGTATCCGATCGCCGTCGTCGTGGTTGCGGTGATCGTGACGTCGATCTT
GCTGATTTTCGTGGTGCCGACCTTCGAGGACCTGTTCAAGAGTTTCGGCGCCGATCTGCCGGCCTTCACCCAGATGGTGA
TCAATCTTTCGCGCTGGCTGCGGGACTGGTGGTACGTGGTGTTCGGCTCGCTGGGCGTAGCCACGGCTGCATTCGTGAAG
GCCCGGCAGCGCTCGCCGGCGTTCAACCATCTTCTGGACCGGCTGGCCTTGGGGCTGCCGGTCGTCGGTGCCATTTTGCA
CAAGGCTGCCATCGCCCGCTTCGCGAGGACATTGTCTACCATGTCCGCAGCCGGCGTGCCCCTGGTCGAGGCACTGCAAT
CGGTGGCAGGGGCGACCGGAAACATCATTTATGGCGACGTGGTTATGCGAATGCGCGAAGAGGTATCCATCGGCCAACAG
CTCCAGATGTCGATGCGCCAGGCCAACCTATTCCCCAACATGGTGGTGCAGATGGTGGCGATTGGTGAGGAGTCGGGGGC
GCTCGACAGCATGCTGTCCAAAGTCGCCGATTTCTACGAAGAAGAAGTGGACAACGCCGTGGATTCGTTGAGCAGCCTGC
TCGAACCCTTGATCATGGTGGTTCTGGGCATCTTGGTCGGGGGGCTGGTGATCGCCATGTATCTGCCGATTTTCAAACTG
GGTTCCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

62.626

98.02

0.614
  pilC Pseudomonas stutzeri DSM 10701

60.25

99.01

0.597
  pilC Acinetobacter baylyi ADP1

58.065

99.752

0.579
  pilC Acinetobacter baumannii D1279779

58.186

98.267

0.572
  pilG Neisseria meningitidis 44/76-A

46.851

98.267

0.46
  pilG Neisseria gonorrhoeae MS11

46.851

98.267

0.46
  pilC Vibrio campbellii strain DS40M4

44.11

98.762

0.436
  pilC Vibrio cholerae strain A1552

43.434

98.02

0.426
  pilC Thermus thermophilus HB27

40.602

98.762

0.401