Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N4J17_RS16325 Genome accession   NZ_CP104311
Coordinates   3384196..3385911 (-) Length   571 a.a.
NCBI ID   WP_198322881.1    Uniprot ID   -
Organism   Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3379196..3390911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4J17_RS16300 (N4J17_16310) - 3380385..3380600 (-) 216 WP_198322876.1 DNA gyrase inhibitor YacG -
  N4J17_RS16305 (N4J17_16315) zapD 3380613..3381326 (-) 714 WP_338457616.1 cell division protein ZapD -
  N4J17_RS16310 (N4J17_16320) coaE 3381481..3382077 (-) 597 WP_198322878.1 dephospho-CoA kinase -
  N4J17_RS16315 (N4J17_16325) pilD 3382085..3382930 (-) 846 WP_277458359.1 A24 family peptidase Machinery gene
  N4J17_RS16320 (N4J17_16330) pilC 3382977..3384191 (-) 1215 WP_198322880.1 type II secretion system F family protein Machinery gene
  N4J17_RS16325 (N4J17_16335) pilB 3384196..3385911 (-) 1716 WP_198322881.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N4J17_RS16330 (N4J17_16340) - 3385961..3388726 (-) 2766 WP_198322882.1 valine--tRNA ligase -
  N4J17_RS16335 (N4J17_16345) - 3388813..3389244 (-) 432 WP_198322883.1 DNA polymerase III subunit chi -
  N4J17_RS16340 (N4J17_16350) - 3389241..3390734 (-) 1494 WP_198322884.1 leucyl aminopeptidase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63157.87 Da        Isoelectric Point: 5.5367

>NTDB_id=728582 N4J17_RS16325 WP_198322881.1 3384196..3385911(-) (pilB) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
MAAVSGGQQLSGLAKSLVKAQLLSEAEAQAYFEDARRQNTPFVSYLVTNKILDGLQIAQVAHQEFGLPLLDLDAIVFDIP
PTQWVSEKLIRRHHILPLLRRGNHLFVAVSDPTNFLGLDEITFQTRLVTDCILVEENKLSRCIEVVLDAADTSLKQLLEE
DLDSLQITAGDDEGPHAEPEISGIDDAPIVRFVNRLLLDAVRKGASDIHIEPYEKFLRIRFRHDGILHEVANPPSALGVK
ICARIKVMSRMDIAEHRIPQDGRIKMTLSRSRAIDFRVNTCPTLFGEKIVLRILDPGSAQIGVEMLGFEPEQRDNFLRAL
EKPYGMILVTGPTGSGKTVTLYTGLNLLNRVETNISTVEDPVEITVPGINQVNVNYKTGLTFAEALRAFLRQDPDVIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIASSVLLIMAQRLARRLCPHCKREEKVPPEVLLA
AGFREEDIGKFMLYGPAGCDQCVKGYKGRVGIYQVMPISEEINRIILEGGNVVALTEQAHAEGIADLRESGLKKVKAGIT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=728582 N4J17_RS16325 WP_198322881.1 3384196..3385911(-) (pilB) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
ATGGCGGCAGTGTCCGGCGGCCAGCAGCTGAGCGGTCTGGCGAAAAGCCTGGTCAAGGCCCAGTTGCTTTCGGAGGCGGA
GGCTCAGGCGTACTTCGAGGATGCGCGCCGGCAGAACACCCCTTTCGTCAGCTATCTCGTCACCAACAAGATCCTCGACG
GCCTCCAGATCGCCCAGGTCGCCCACCAGGAGTTCGGCCTACCCCTGCTCGACCTGGACGCAATCGTGTTCGACATCCCC
CCCACCCAATGGGTCAGCGAGAAGCTGATACGCCGCCATCACATTCTGCCCTTGCTGCGACGGGGGAATCACCTCTTCGT
CGCGGTCTCGGATCCGACCAATTTTCTGGGGCTGGATGAAATCACTTTCCAGACCCGTCTCGTCACCGACTGTATTCTGG
TCGAGGAGAATAAGCTTTCGCGCTGCATCGAGGTGGTCCTGGATGCGGCCGACACCAGCCTCAAGCAACTGCTCGAAGAG
GACCTGGACAGTCTCCAGATCACCGCGGGGGACGACGAAGGCCCGCACGCCGAACCGGAAATTTCCGGCATCGACGACGC
GCCGATCGTGCGTTTCGTCAACCGGCTCTTATTGGACGCGGTGCGGAAAGGGGCTTCGGACATTCATATCGAGCCCTACG
AAAAATTCCTGCGCATTCGCTTCCGACACGACGGCATCCTGCACGAAGTGGCGAATCCGCCATCGGCGCTGGGCGTCAAG
ATCTGCGCTCGCATCAAAGTGATGTCGCGCATGGATATCGCGGAACACCGCATTCCCCAGGACGGACGCATCAAGATGAC
GCTGTCGCGCTCCCGCGCTATCGATTTCCGCGTCAATACCTGTCCGACGCTGTTCGGGGAAAAGATCGTGCTGCGTATCC
TCGATCCGGGTTCGGCCCAGATCGGCGTTGAAATGCTGGGATTCGAACCCGAGCAGCGAGACAATTTCTTGCGCGCACTG
GAAAAGCCCTATGGCATGATTCTGGTCACCGGCCCCACCGGCAGTGGCAAAACCGTCACGCTCTACACCGGTCTGAACCT
CCTCAACCGCGTCGAAACCAACATCTCTACGGTCGAGGACCCGGTCGAAATCACGGTGCCGGGGATCAACCAGGTCAACG
TCAACTACAAGACCGGCCTCACTTTCGCCGAGGCGCTGCGGGCGTTTCTGCGTCAGGATCCCGACGTCATCATGGTCGGC
GAGATCCGCGACCTGGAAACCGCCGAAATCGCCGTCAAGGCCGCTCAGACCGGACATCTGGTGCTTTCGACCCTGCATAC
CAACGATGCGCCGCAGACCTTGAACCGTCTGATGCAGATGGGGATCCCGGCGTTCAACATCGCCTCCTCCGTGCTCCTGA
TCATGGCACAGCGGCTGGCCCGCCGGCTTTGTCCGCATTGCAAGCGGGAGGAGAAAGTACCGCCCGAGGTCCTGCTCGCC
GCCGGCTTCCGGGAGGAAGACATCGGCAAATTCATGCTTTACGGACCGGCGGGTTGTGATCAGTGCGTGAAAGGCTACAA
GGGGCGGGTCGGCATATACCAAGTCATGCCGATTTCGGAAGAAATCAACCGCATCATCCTGGAAGGCGGGAATGTGGTGG
CCCTGACCGAACAGGCGCATGCGGAAGGTATCGCGGATCTGAGGGAGTCTGGCTTGAAGAAGGTGAAGGCGGGCATCACC
AGCCTCGAGGAAATCGACCGCGTGACGAGGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56

100

0.564
  pilB Acinetobacter baylyi ADP1

52.055

100

0.532
  pilB Acinetobacter baumannii D1279779

51.295

100

0.52
  pilB Vibrio cholerae strain A1552

49.023

98.599

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

47.865

98.424

0.471
  pilF Neisseria gonorrhoeae MS11

47.695

98.774

0.471
  pilB Vibrio campbellii strain DS40M4

46.892

98.599

0.462
  pilF Thermus thermophilus HB27

38.146

98.249

0.375