Detailed information NTG0728580

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N4J17_RS16315
• Genome accession: NZ_CP104311
• Coordinates: 3382085..3382930 (-)
• Length: 281 a.a.
• NCBI ID: WP_277458359.1
• Uniprot ID: -
• Organism: Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3377085..3387930

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4J17_RS16280 (N4J17_16290)	-	3377233..3377808 (-)	576	WP_198322906.1	RNA pyrophosphohydrolase	-
N4J17_RS16285 (N4J17_16295)	-	3378002..3378664 (+)	663	WP_198322873.1	HAD family hydrolase	-
N4J17_RS16290 (N4J17_16300)	-	3378673..3378903 (-)	231	WP_198322874.1	sulfurtransferase TusA family protein	-
N4J17_RS16295 (N4J17_16305)	cysG	3378951..3380375 (+)	1425	WP_198322875.1	siroheme synthase CysG	-
N4J17_RS16300 (N4J17_16310)	-	3380385..3380600 (-)	216	WP_198322876.1	DNA gyrase inhibitor YacG	-
N4J17_RS16305 (N4J17_16315)	zapD	3380613..3381326 (-)	714	WP_338457616.1	cell division protein ZapD	-
N4J17_RS16310 (N4J17_16320)	coaE	3381481..3382077 (-)	597	WP_198322878.1	dephospho-CoA kinase	-
N4J17_RS16315 (N4J17_16325)	pilD	3382085..3382930 (-)	846	WP_277458359.1	A24 family peptidase	Machinery gene
N4J17_RS16320 (N4J17_16330)	pilC	3382977..3384191 (-)	1215	WP_198322880.1	type II secretion system F family protein	Machinery gene
N4J17_RS16325 (N4J17_16335)	pilB	3384196..3385911 (-)	1716	WP_198322881.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 30925.47 Da
• Isoelectric Point: 7.2780

>NTDB_id=728580 N4J17_RS16315 WP_277458359.1 3382085..3382930(-) (pilD) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
MIWVWGLFGLIIGSFLNVVIHRLPLMMEQAWRRECREFLEPGGGHPSVEPEYDLWRPGSQCPHCRAPVRFWQNVPILSYL
WLRGRCAACGAAISWRYPLVEALTGMLFATLAWRFGASWQALAASGLTAALIALAFIDIDHLVLPDDITLPLLWLGLLLN
AQSLFCNLETALYGAVAGYLLLWTVHRGFLLLTGREGMGFGDFKLLAMLGAWMGWPMLPVIILFSSISGAVIGSIVLAVG
GKERNTPIPFGPFLAAGGWIALLWGNTLNDAYWRWATAGGG

Nucleotide

• Length: 846 bp

>NTDB_id=728580 N4J17_RS16315 WP_277458359.1 3382085..3382930(-) (pilD) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
TTGATCTGGGTTTGGGGCTTGTTCGGACTGATCATCGGCAGTTTCCTCAACGTAGTTATCCATCGTCTACCGCTCATGAT
GGAGCAGGCTTGGCGCAGGGAGTGCCGGGAGTTTCTCGAGCCTGGCGGCGGGCATCCGTCCGTGGAGCCCGAATACGACC
TGTGGAGACCCGGCTCGCAGTGTCCACACTGCAGGGCGCCGGTCCGGTTCTGGCAGAATGTCCCTATACTCAGCTATCTT
TGGCTGCGCGGCCGCTGTGCCGCTTGCGGCGCCGCAATCTCCTGGCGATATCCTCTGGTCGAGGCGCTCACGGGCATGCT
GTTCGCGACGCTGGCCTGGCGTTTCGGCGCGTCCTGGCAGGCCCTGGCTGCGTCCGGTCTGACAGCGGCTTTGATCGCCC
TGGCTTTCATCGATATTGATCACCTCGTGCTCCCGGACGACATCACCCTTCCCCTGCTGTGGCTAGGCCTGCTACTGAAC
GCCCAGAGTCTGTTCTGCAACCTGGAGACGGCGCTGTACGGCGCCGTCGCTGGCTATCTCCTGCTATGGACGGTGCATCG
CGGCTTTCTGCTGCTGACCGGTCGGGAGGGCATGGGGTTCGGTGACTTCAAGCTGCTCGCCATGCTCGGCGCCTGGATGG
GCTGGCCCATGCTGCCGGTCATTATCCTGTTCTCGTCCATCTCGGGAGCGGTGATCGGTTCGATTGTGCTGGCGGTCGGC
GGCAAGGAGAGGAATACGCCGATTCCATTCGGGCCGTTTCTGGCGGCGGGTGGCTGGATTGCCCTGTTGTGGGGAAATAC
GCTCAACGACGCCTATTGGCGCTGGGCTACTGCCGGAGGAGGCTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (7-115)

• Type IV leader peptidase family (Peptidase_A24) (128-233)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.746	95.374	0.484
pilD	Neisseria gonorrhoeae MS11	48.897	96.797	0.473
pilD	Acinetobacter baumannii D1279779	47.761	95.374	0.456
pilD	Acinetobacter nosocomialis M2	47.761	95.374	0.456
pilD	Vibrio campbellii strain DS40M4	47.388	95.374	0.452