Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KU43P_RS01545 Genome accession   NZ_AP019365
Coordinates   323848..325092 (+) Length   414 a.a.
NCBI ID   WP_317660745.1    Uniprot ID   -
Organism   Pseudomonas sp. KU43P     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318848..330092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU43P_RS01520 (KU43P_03010) - 318879..321329 (-) 2451 WP_411567242.1 penicillin-binding protein 1A -
  KU43P_RS01525 (KU43P_03020) pilM 321512..322369 (+) 858 WP_317660741.1 type IV pilus biogenesis protein PilM -
  KU43P_RS01530 (KU43P_03030) - 322366..322902 (+) 537 WP_317660742.1 PilN domain-containing protein -
  KU43P_RS01535 (KU43P_03040) pilO 322899..323402 (+) 504 WP_317660743.1 type 4a pilus biogenesis protein PilO -
  KU43P_RS01540 (KU43P_03050) - 323436..323894 (+) 459 WP_317660744.1 pilus assembly protein PilP -
  KU43P_RS01545 (KU43P_03060) pilQ 323848..325092 (+) 1245 WP_317660745.1 type IV pilus secretin PilQ Machinery gene
  KU43P_RS01550 (KU43P_03070) aroK 325097..325615 (+) 519 WP_317660746.1 shikimate kinase AroK -
  KU43P_RS01555 (KU43P_03080) aroB 325710..326807 (+) 1098 WP_317660747.1 3-dehydroquinate synthase -
  KU43P_RS01560 (KU43P_03090) - 326823..328436 (+) 1614 WP_317660748.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 414 a.a.        Molecular weight: 44775.13 Da        Isoelectric Point: 4.6912

>NTDB_id=72420 KU43P_RS01545 WP_317660745.1 323848..325092(+) (pilQ) [Pseudomonas sp. KU43P]
MAAMLIPVHGLSASYKGEPVSLNFQDVEVRSVLQVLADYAGINLVASDQVQGNITLRLQDVPWDQALALVLRSKGLVRRE
EGNVLLVAPAGELHEQSFGDSFGQALDAQLLPLRRELFAIHHADAAELAEVLLAALADDGILAGRGSLSVDARTNTLVAH
QPEERLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGALHEEVARMGKDVFVDLGVERSGSAVGLGLLRGD
VLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSLSGATSVSFREASLSLEVTPQITPDGKVIMTVKV
TKDEPDFVNALNDVPPIRKNEVNAKVRVADGDTIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFL
TPRIMSDQAIAVSR

Nucleotide


Download         Length: 1245 bp        

>NTDB_id=72420 KU43P_RS01545 WP_317660745.1 323848..325092(+) (pilQ) [Pseudomonas sp. KU43P]
ATGGCAGCGATGCTGATACCGGTTCACGGGCTGTCAGCCTCTTATAAGGGGGAGCCGGTTTCGTTGAATTTTCAGGATGT
GGAGGTGCGCTCGGTGCTTCAGGTCCTGGCGGACTATGCGGGCATCAACCTGGTGGCCAGTGACCAGGTGCAAGGCAACA
TCACCCTGCGCTTGCAGGATGTGCCATGGGACCAAGCCTTGGCACTGGTGCTGCGCAGCAAAGGCCTGGTGCGGCGCGAA
GAGGGCAACGTGCTGTTGGTGGCGCCTGCGGGTGAGCTGCACGAGCAATCTTTCGGCGATTCGTTCGGGCAGGCACTCGA
TGCTCAGTTACTGCCTTTGCGCAGGGAGCTGTTTGCGATTCATCACGCTGATGCCGCCGAGCTTGCGGAGGTATTGCTGG
CAGCGCTGGCGGACGATGGCATCCTCGCGGGGCGCGGTAGCCTGAGCGTCGATGCGCGTACCAATACGCTGGTAGCGCAC
CAACCCGAGGAGCGGCTGGCCGAGTTGCGCCAACTGGTGGCGCAACTGGATGTGCCGGTGCGCCAGGTGGCAATCGAGGC
GCGCATTGTCGAAGCCAATGTCGATTATGAAAAAAGCCTAGGCGTGCGTTGGGGTGGCGCCTTGCATGAAGAAGTCGCGC
GTATGGGCAAGGATGTGTTCGTCGACCTTGGCGTGGAACGCTCCGGTTCAGCGGTAGGGTTGGGCCTGTTGCGGGGCGAT
GTATTGCTGGACCTTGAGCTCAGCGCCATGGAAAAGAGCGGCAATGGCGAGATCATTTCCCAGCCCAAGGTGGTTACCGC
TGACAAGGAAACGGCGAGGATCCTCAAGGGAACGGAGGTGCCTTACCAGGAAACCAGCTTGAGCGGCGCTACCTCGGTTT
CTTTCAGGGAGGCTTCGCTGTCGCTAGAAGTTACCCCGCAGATCACCCCGGACGGCAAGGTCATCATGACCGTCAAGGTG
ACCAAGGACGAGCCGGACTTCGTCAATGCCTTGAACGACGTGCCGCCGATTCGCAAGAACGAGGTCAACGCCAAGGTGCG
TGTGGCCGACGGTGACACCATCGTCATTGGTGGCGTGTACTCGACCACGCAAAACAATGTGGTCGACAAGGTGCCATTTT
TCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTG
ACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(117-182)

Secretin and TonB N terminus short domain (STN)

(43-89)

Bacterial type II and III secretion system protein (Secretin)

(249-405)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.039

100

0.623
  comQ Acinetobacter baylyi ADP1

42

100

0.457
  pilQ Acinetobacter baumannii D1279779

39.61

100

0.442
  pilQ Acinetobacter baumannii strain A118

39.61

100

0.442
  pilQ Legionella pneumophila strain ERS1305867

42.344

100

0.428
  pilQ Legionella pneumophila strain Lp02

42.344

100

0.428
  pilQ Vibrio campbellii strain DS40M4

40.094

100

0.411
  pilQ Vibrio cholerae strain A1552

39.904

100

0.401
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.904

100

0.401
  pilQ Neisseria gonorrhoeae strain FA1090

36.3

100

0.374
  pilQ Neisseria meningitidis M1080

35.363

100

0.365

Multiple sequence alignment