Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NWE71_RS17185 Genome accession   NZ_CP103291
Coordinates   3557626..3558282 (-) Length   218 a.a.
NCBI ID   WP_309520645.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans strain NS1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3516367..3557652 3557626..3558282 flank -26


Gene organization within MGE regions


Location: 3516367..3558282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWE71_RS16860 - 3516559..3517209 (-) 651 WP_258831432.1 hypothetical protein -
  NWE71_RS16865 - 3517115..3518308 (-) 1194 WP_258831433.1 FtsK/SpoIIIE domain-containing protein -
  NWE71_RS16870 - 3518301..3518531 (-) 231 WP_258831434.1 hypothetical protein -
  NWE71_RS16875 - 3518712..3518894 (-) 183 WP_230301407.1 hypothetical protein -
  NWE71_RS16880 - 3519039..3519233 (+) 195 WP_258831435.1 helix-turn-helix transcriptional regulator -
  NWE71_RS16885 - 3519291..3519893 (+) 603 WP_258831436.1 hypothetical protein -
  NWE71_RS16890 - 3519894..3520142 (+) 249 WP_258831437.1 hypothetical protein -
  NWE71_RS16895 - 3520183..3521328 (-) 1146 WP_258831438.1 peptidoglycan-binding protein -
  NWE71_RS16900 - 3521380..3521652 (-) 273 WP_374116789.1 holin -
  NWE71_RS16905 - 3521657..3521998 (-) 342 WP_258831439.1 hypothetical protein -
  NWE71_RS16910 - 3522233..3522445 (+) 213 WP_258831440.1 hypothetical protein -
  NWE71_RS16915 - 3522540..3523574 (-) 1035 WP_258831441.1 acyltransferase -
  NWE71_RS16920 - 3523671..3525887 (-) 2217 WP_258831442.1 BppU family phage baseplate upper protein -
  NWE71_RS16925 - 3525923..3528484 (-) 2562 WP_258831443.1 phage tail spike protein -
  NWE71_RS16930 - 3528497..3529348 (-) 852 WP_258831444.1 phage tail family protein -
  NWE71_RS16935 - 3529357..3532200 (-) 2844 WP_258831445.1 hypothetical protein -
  NWE71_RS16940 - 3532201..3532443 (-) 243 WP_230301421.1 hypothetical protein -
  NWE71_RS16945 - 3532533..3532988 (-) 456 WP_258831446.1 tail assembly chaperone -
  NWE71_RS16950 - 3533049..3533546 (-) 498 WP_258831447.1 phage major tail protein, TP901-1 family -
  NWE71_RS16955 - 3533558..3533965 (-) 408 WP_258831448.1 DUF3168 domain-containing protein -
  NWE71_RS16960 - 3533971..3534378 (-) 408 WP_258831449.1 HK97-gp10 family putative phage morphogenesis protein -
  NWE71_RS16965 - 3534375..3534689 (-) 315 WP_258831450.1 phage head closure protein -
  NWE71_RS16970 - 3534689..3534982 (-) 294 WP_258831451.1 phage head-tail connector protein -
  NWE71_RS16975 - 3535132..3535944 (-) 813 WP_258831452.1 N4-gp56 family major capsid protein -
  NWE71_RS16980 - 3535957..3536568 (-) 612 WP_258831453.1 phage scaffolding protein -
  NWE71_RS16985 - 3536661..3537575 (-) 915 WP_258831454.1 phage minor head protein -
  NWE71_RS16990 - 3537572..3539131 (-) 1560 WP_258831455.1 phage portal protein -
  NWE71_RS16995 - 3539145..3540404 (-) 1260 WP_258831456.1 PBSX family phage terminase large subunit -
  NWE71_RS17000 - 3540394..3540837 (-) 444 WP_258831457.1 terminase small subunit -
  NWE71_RS17005 - 3540871..3540999 (-) 129 WP_258831458.1 hypothetical protein -
  NWE71_RS17010 - 3541052..3541183 (-) 132 WP_258831459.1 hypothetical protein -
  NWE71_RS17015 - 3541214..3541420 (-) 207 WP_258831460.1 hypothetical protein -
  NWE71_RS17020 - 3541491..3541994 (-) 504 WP_258831461.1 hypothetical protein -
  NWE71_RS17025 - 3542275..3542709 (-) 435 WP_258831462.1 hypothetical protein -
  NWE71_RS17030 - 3542741..3543223 (-) 483 WP_258831463.1 hypothetical protein -
  NWE71_RS17035 - 3543256..3543657 (-) 402 WP_258831464.1 hypothetical protein -
  NWE71_RS17040 - 3543654..3543833 (-) 180 WP_258831465.1 hypothetical protein -
  NWE71_RS17045 - 3543852..3544343 (-) 492 WP_258831466.1 hypothetical protein -
  NWE71_RS17050 - 3544340..3544480 (-) 141 WP_258831467.1 hypothetical protein -
  NWE71_RS17055 - 3544473..3544805 (-) 333 WP_258831468.1 nucleoside triphosphate pyrophosphohydrolase family protein -
  NWE71_RS17060 - 3544806..3545618 (-) 813 WP_258831469.1 hypothetical protein -
  NWE71_RS17065 ssbA 3545629..3546099 (-) 471 WP_258831470.1 single-stranded DNA-binding protein Machinery gene
  NWE71_RS17070 - 3546264..3546758 (-) 495 WP_258831471.1 HNH endonuclease -
  NWE71_RS17075 - 3546758..3547675 (-) 918 WP_258831472.1 hypothetical protein -
  NWE71_RS17080 - 3547708..3547977 (-) 270 WP_258831473.1 hypothetical protein -
  NWE71_RS17085 - 3548115..3548381 (-) 267 WP_258831474.1 hypothetical protein -
  NWE71_RS17090 - 3548387..3548695 (-) 309 WP_258831475.1 hypothetical protein -
  NWE71_RS17095 - 3548713..3549537 (-) 825 WP_258831476.1 ATP-binding protein -
  NWE71_RS17100 - 3549497..3550267 (-) 771 WP_258831477.1 phage replisome organizer N-terminal domain-containing protein -
  NWE71_RS17105 - 3550368..3550982 (-) 615 WP_258831478.1 hypothetical protein -
  NWE71_RS17110 - 3551016..3551231 (-) 216 WP_258831479.1 helix-turn-helix transcriptional regulator -
  NWE71_RS17115 - 3551442..3551660 (+) 219 WP_258831480.1 helix-turn-helix transcriptional regulator -
  NWE71_RS17120 - 3551727..3551921 (+) 195 WP_258831481.1 hypothetical protein -
  NWE71_RS17125 - 3551987..3552271 (-) 285 WP_258831482.1 hypothetical protein -
  NWE71_RS17130 - 3552341..3552583 (-) 243 WP_258831483.1 hypothetical protein -
  NWE71_RS17135 - 3552642..3552845 (-) 204 WP_258831484.1 hypothetical protein -
  NWE71_RS17140 - 3552976..3553767 (-) 792 WP_258831485.1 PD-(D/E)XK nuclease-like domain-containing protein -
  NWE71_RS17145 - 3553748..3554578 (-) 831 WP_258831486.1 hypothetical protein -
  NWE71_RS17150 - 3554656..3554859 (-) 204 WP_258831487.1 hypothetical protein -
  NWE71_RS17155 - 3554856..3555152 (-) 297 WP_258831488.1 hypothetical protein -
  NWE71_RS17160 - 3555199..3555387 (-) 189 WP_241589791.1 helix-turn-helix domain-containing protein -
  NWE71_RS17165 - 3555431..3555616 (-) 186 WP_258831489.1 helix-turn-helix transcriptional regulator -
  NWE71_RS17170 - 3555817..3556152 (+) 336 WP_258831490.1 helix-turn-helix domain-containing protein -
  NWE71_RS17175 - 3556341..3556472 (+) 132 WP_258831491.1 hypothetical protein -
  NWE71_RS17180 - 3556441..3557562 (+) 1122 WP_258831492.1 site-specific integrase -
  NWE71_RS17185 comGA 3557626..3558282 (-) 657 WP_309520645.1 ATPase, T2SS/T4P/T4SS family Machinery gene

Sequence

Protein


Download         Length: 218 a.a.        Molecular weight: 24319.38 Da        Isoelectric Point: 7.6497

>NTDB_id=721730 NWE71_RS17185 WP_309520645.1 3557626..3558282(-) (comGA) [Peribacillus frigoritolerans strain NS1]
MNELISIEKTAEKILTRAIVESASDIHIFFRGEGPHIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSG
AITINLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSL
LHHANEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVKA

Nucleotide


Download         Length: 657 bp        

>NTDB_id=721730 NWE71_RS17185 WP_309520645.1 3557626..3558282(-) (comGA) [Peribacillus frigoritolerans strain NS1]
ATGAATGAATTGATATCGATTGAAAAGACCGCAGAAAAAATACTAACCCGTGCCATCGTGGAATCGGCATCGGATATCCA
CATTTTTTTTCGCGGAGAGGGGCCTCACATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACATTATCATTCT
TTGAAGCTGAAAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGT
GCGATCACAATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGT
CATCCGCTTAATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTG
CACTCCTGAAGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACACTATATTCCCTG
CTTCACCACGCCAATGAGATGATCAATCGAAATATCATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTATT
GCAAGTCCAAATCAATGAAAAGGCGGGCATTACGTATTCAGTCGGCCTAAAAGCGGTTCTGAGGCATGACCCGGACGTGA
TCATGGTTAAAGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.82

96.789

0.56
  pilB Vibrio cholerae strain A1552

36.564

100

0.381
  pilB Legionella pneumophila strain ERS1305867

38.426

99.083

0.381
  pilB Glaesserella parasuis strain SC1401

40.686

93.578

0.381
  pilB Haemophilus influenzae 86-028NP

39.423

95.413

0.376
  pilB Acinetobacter baylyi ADP1

38.679

97.248

0.376
  pilB Acinetobacter baumannii D1279779

38.208

97.248

0.372
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.5

91.743

0.372
  pilF Neisseria gonorrhoeae MS11

38.278

95.872

0.367
  pilB Haemophilus influenzae Rd KW20

38.462

95.413

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

38.835

94.495

0.367