Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EL334_RS10565 Genome accession   NZ_AP019192
Coordinates   1936595..1937611 (-) Length   338 a.a.
NCBI ID   WP_013193332.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain ASP0581     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1931595..1942611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL334_RS12550 - 1931679..1931810 (-) 132 WP_000768904.1 hypothetical protein -
  EL334_RS10525 (ASP0581_19530) - 1931811..1933001 (-) 1191 WP_000167766.1 acetate kinase -
  EL334_RS10530 (ASP0581_19540) comYH 1933052..1934005 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  EL334_RS10535 (ASP0581_19550) - 1934066..1934653 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  EL334_RS10540 (ASP0581_19560) comGG/cglG 1934790..1935203 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  EL334_RS10545 comGF/cglF 1935181..1935642 (-) 462 WP_033705217.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL334_RS10550 (ASP0581_19580) comGE/cglE 1935605..1935907 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  EL334_RS10555 (ASP0581_19590) comGD/cglD 1935870..1936274 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL334_RS10560 (ASP0581_19600) comGC/cglC 1936267..1936593 (-) 327 WP_000738638.1 comG operon protein ComGC Machinery gene
  EL334_RS10565 (ASP0581_19610) comGB/cglB 1936595..1937611 (-) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL334_RS10570 (ASP0581_19620) comGA/cglA/cilD 1937559..1938500 (-) 942 WP_126432103.1 competence type IV pilus ATPase ComGA Machinery gene
  EL334_RS10575 (ASP0581_19630) - 1938576..1938941 (-) 366 WP_000286415.1 DUF1033 family protein -
  EL334_RS10580 (ASP0581_19640) - 1939092..1940150 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  EL334_RS10585 (ASP0581_19650) nagA 1940313..1941464 (-) 1152 WP_033705218.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38524.60 Da        Isoelectric Point: 9.6162

>NTDB_id=72172 EL334_RS10565 WP_013193332.1 1936595..1937611(-) (comGB/cglB) [Streptococcus pneumoniae strain ASP0581]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=72172 EL334_RS10565 WP_013193332.1 1936595..1937611(-) (comGB/cglB) [Streptococcus pneumoniae strain ASP0581]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTCCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae D39

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae R6

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae TIGR4

99.112

100

0.991
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment