Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRZ30_RS09910 Genome accession   NZ_CP102367
Coordinates   2183015..2184124 (-) Length   369 a.a.
NCBI ID   WP_201912858.1    Uniprot ID   -
Organism   Aeromonas jandaei strain BC17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2178015..2189124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ30_RS09880 (NRZ30_09880) - 2178586..2179593 (-) 1008 WP_268018237.1 Fe(3+) ABC transporter substrate-binding protein -
  NRZ30_RS09885 (NRZ30_09885) glnK 2179787..2180125 (-) 339 WP_268018162.1 P-II family nitrogen regulator -
  NRZ30_RS09890 (NRZ30_09890) arfB 2180327..2180743 (+) 417 WP_033114047.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  NRZ30_RS09895 (NRZ30_09895) - 2180765..2181256 (+) 492 WP_268018163.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  NRZ30_RS09900 (NRZ30_09900) - 2181571..2181999 (-) 429 WP_043849992.1 hypothetical protein -
  NRZ30_RS09905 (NRZ30_09905) yaaA 2182194..2182967 (-) 774 WP_268018164.1 peroxide stress protein YaaA -
  NRZ30_RS09910 (NRZ30_09910) pilU 2183015..2184124 (-) 1110 WP_201912858.1 type IVa pilus ATPase TapU Machinery gene
  NRZ30_RS09915 (NRZ30_09915) pilT 2184153..2185187 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  NRZ30_RS09920 (NRZ30_09920) - 2185227..2185928 (+) 702 WP_156853921.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRZ30_RS09925 (NRZ30_09925) proC 2185995..2186822 (+) 828 WP_268018165.1 pyrroline-5-carboxylate reductase -
  NRZ30_RS09930 (NRZ30_09930) - 2186834..2187385 (+) 552 WP_042031658.1 YggT family protein -
  NRZ30_RS09935 (NRZ30_09935) yggU 2187385..2187684 (+) 300 WP_033114039.1 DUF167 family protein YggU -
  NRZ30_RS09940 (NRZ30_09940) - 2187714..2188133 (+) 420 WP_268018166.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41128.22 Da        Isoelectric Point: 6.4424

>NTDB_id=716524 NRZ30_RS09910 WP_201912858.1 2183015..2184124(-) (pilU) [Aeromonas jandaei strain BC17]
MNLDELLGQLVEKKGSDLFVTVGTPPTLKVNGHLLSLGGEALDKKAALTLVRETLSPEHFERYIRTREANYAIHRETLGR
FRVSAFWQQELPGMVVRRIETRIPTFDDLQLPVILQEVALAKRGLVLFVGATGAGKSTTQATMIGYRNQHADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSSETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLKAIVAQQLVPSVDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQS
LFNLFCSGQIGYSEALAHADSANDLRLLIKLSGREQLGMGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=716524 NRZ30_RS09910 WP_201912858.1 2183015..2184124(-) (pilU) [Aeromonas jandaei strain BC17]
ATGAATCTGGATGAACTGCTGGGCCAGCTGGTAGAGAAAAAAGGGTCGGATCTCTTTGTGACGGTGGGGACGCCCCCCAC
CCTCAAGGTGAACGGCCATCTGCTCTCTTTGGGAGGCGAAGCGCTCGATAAAAAAGCTGCTCTTACGCTGGTGAGGGAGA
CCCTGAGCCCCGAGCATTTCGAACGCTATATCCGCACCAGGGAGGCCAACTACGCCATTCACCGCGAAACTTTGGGCCGG
TTTCGGGTCAGCGCTTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTTAGGCGTATCGAGACCCGGATCCCTACCTTCGA
TGACCTGCAATTGCCCGTGATCCTGCAGGAGGTCGCATTGGCCAAGCGGGGACTGGTGCTGTTTGTCGGCGCCACCGGTG
CGGGTAAATCGACCACCCAAGCGACCATGATTGGTTATCGCAACCAGCATGCCGACGGCCATATTCTGACGGTGGAAGAT
CCGGTGGAGTTTGTCCATCAGCATGGCCGCAGCCTGATCACCCAGCGTGAGGTGGGGATCGATACCGAGTCGTTCGATGT
AGCGTTGAAAAGCTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCAGAAGCTCTGAGACCATGGAGTTTG
CCCTGCAATTTGCCGAGACCGGCCACCTCTGCCTCGCGACTCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATT
CTGCATCTGGTGCCGCAGGAGAAGCATCGTCAGTTCCTGTTCGATCTCTCCTTCAACCTCAAGGCCATTGTCGCCCAGCA
ACTGGTGCCGAGCGTGGATGGCAAGCGGCGCTGCGCCGCATTCGAGATCCTGCTCAACACCCCGCTTATCACCGACATTA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAATCGACCGAGCTTGGCATGCAAACCTTCGATCAATCG
CTCTTCAACCTGTTCTGCTCCGGCCAGATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGGCT
GCTGATCAAGCTTTCCGGTCGTGAGCAGCTGGGAATGGGAACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.783

99.729

0.596
  pilU Pseudomonas stutzeri DSM 10701

60.114

95.122

0.572
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.444

97.561

0.385
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363