Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRK98_RS00265 Genome accession   NZ_CP102325
Coordinates   47688..48797 (-) Length   369 a.a.
NCBI ID   WP_019838905.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 42688..53797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRK98_RS00245 (NRK98_00245) brnQ 43145..44452 (-) 1308 WP_005305030.1 branched-chain amino acid transport system II carrier protein -
  NRK98_RS00250 (NRK98_00250) - 44644..45354 (-) 711 WP_268028050.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NRK98_RS00255 (NRK98_00255) srmB 45483..46706 (+) 1224 WP_268028051.1 ATP-dependent RNA helicase SrmB -
  NRK98_RS00260 (NRK98_00260) yaaA 46883..47656 (-) 774 WP_017783834.1 peroxide stress protein YaaA -
  NRK98_RS00265 (NRK98_00265) pilU 47688..48797 (-) 1110 WP_019838905.1 type IVa pilus ATPase TapU Machinery gene
  NRK98_RS00270 (NRK98_00270) pilT 48826..49860 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  NRK98_RS00275 (NRK98_00275) - 49900..50601 (+) 702 WP_010636180.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRK98_RS00280 (NRK98_00280) proC 50662..51486 (+) 825 WP_005305003.1 pyrroline-5-carboxylate reductase -
  NRK98_RS00285 (NRK98_00285) - 51527..52078 (+) 552 WP_017780458.1 YggT family protein -
  NRK98_RS00290 (NRK98_00290) yggU 52078..52377 (+) 300 WP_005304998.1 DUF167 family protein YggU -
  NRK98_RS00295 (NRK98_00295) - 52396..52815 (+) 420 WP_005304995.1 DUF4426 domain-containing protein -
  NRK98_RS00300 (NRK98_00300) - 52879..53190 (-) 312 WP_005304992.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41139.26 Da        Isoelectric Point: 6.4437

>NTDB_id=716100 NRK98_RS00265 WP_019838905.1 47688..48797(-) (pilU) [Aeromonas dhakensis strain BC03]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=716100 NRK98_RS00265 WP_019838905.1 47688..48797(-) (pilU) [Aeromonas dhakensis strain BC03]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAATGGCCACCTGCAGTCACTGGCAGAAGCGCCACTCGACAAGGCAAGGGCACTGGCACTGGTGAAGGAGA
CCCTGAGCCCGGAGCACTTCGAACGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTACTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATATTGCAGGAGATCGCCCTCGCCAAGCGCGGGTTGGTACTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACGGAGTCGTTCGACGT
GGCGCTGAAGAGCTCACTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAATTTGCCGAGACCGGTCACCTCTGTCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTGCCGCAGGAGAAACACCGTCAGTTCCTGTTTGATCTCTCCTTCAATCTGCGCGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCATCGGCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCTGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAACAGCTGGGTTCAGGCACACTCGACAACGTTACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.141

99.729

0.61
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363