Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NQ200_RS17390 Genome accession   NZ_CP102172
Coordinates   3840502..3841611 (+) Length   369 a.a.
NCBI ID   WP_087757149.1    Uniprot ID   -
Organism   Aeromonas salmonicida subsp. masoucida strain wx3-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3835502..3846611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ200_RS17355 - 3836091..3836390 (+) 300 WP_011899099.1 hypothetical protein -
  NQ200_RS17360 - 3836459..3836878 (-) 420 WP_005320482.1 DUF4426 domain-containing protein -
  NQ200_RS17365 yggU 3836897..3837196 (-) 300 WP_005320479.1 DUF167 family protein YggU -
  NQ200_RS17370 - 3837196..3837747 (-) 552 WP_005320477.1 YggT family protein -
  NQ200_RS17375 proC 3837776..3838600 (-) 825 WP_309265706.1 pyrroline-5-carboxylate reductase -
  NQ200_RS17380 - 3838691..3839392 (-) 702 WP_058395315.1 YggS family pyridoxal phosphate-dependent enzyme -
  NQ200_RS17385 pilT 3839432..3840466 (+) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  NQ200_RS17390 pilU 3840502..3841611 (+) 1110 WP_087757149.1 type IVa pilus ATPase TapU Machinery gene
  NQ200_RS17395 yaaA 3841643..3842416 (+) 774 WP_087757148.1 peroxide stress protein YaaA -
  NQ200_RS17400 srmB 3842605..3843828 (-) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  NQ200_RS17405 - 3843957..3844667 (+) 711 WP_096118856.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NQ200_RS17410 brnQ 3844853..3846166 (+) 1314 WP_277735641.1 branched-chain amino acid transport system II carrier protein -
  NQ200_RS17415 - 3846242..3846472 (-) 231 WP_162626231.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41059.10 Da        Isoelectric Point: 6.3717

>NTDB_id=715151 NQ200_RS17390 WP_087757149.1 3840502..3841611(+) (pilU) [Aeromonas salmonicida subsp. masoucida strain wx3-1]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLFSLGSEALDKQSALTLVRETLSPEHFERYIRTKEGNYAIHREALGR
FRVSAFWQQELPGMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=715151 NQ200_RS17390 WP_087757149.1 3840502..3841611(+) (pilU) [Aeromonas salmonicida subsp. masoucida strain wx3-1]
ATGAATCTGGATGCCCTGTTGGCCGAACTGGTCGCACGCAAAGGCTCGGATCTGTTCATCACGGTTGGGACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGTTCTCCCTCGGCAGCGAAGCGCTCGACAAGCAGAGCGCGCTAACCCTGGTCAGGGAAA
CGCTCAGCCCTGAGCATTTCGAGCGCTACATCCGCACCAAGGAGGGTAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTCGGGTGAGTGCCTTCTGGCAGCAGGAGTTGCCGGGCATGGTGGTGCGCCGTATCGAGACCCGCATTCCAACCTTCGA
CGAACTGACCCTGCCGCCCATCTTGCAGGAGGTGGCCATGGCCAAACGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGAA
CGGGCAAGTCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGATGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGGGAAGTGGGCATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCACCTGACGTTATCCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGCCATCTTTGTCTCGCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAACACCGCCAGTTACTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGTCGCCGCCGCTTCGCGGCCTTTGAAGTACTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGTCTCAAGGAGGTGATGACCAAATCCACCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.239

99.729

0.591
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36