Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J73_RS03615 Genome accession   NZ_AP018780
Coordinates   717177..718574 (+) Length   465 a.a.
NCBI ID   WP_005660019.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-IV-1 isolate 13H36     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 712177..723574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J73_RS03590 (CHBNIV1_06790) rsmE 712192..712929 (-) 738 WP_005660011.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J73_RS03595 (CHBNIV1_06800) lnt 712979..714508 (-) 1530 WP_343213443.1 apolipoprotein N-acyltransferase -
  K6J73_RS03600 (CHBNIV1_06810) corC 714531..715430 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J73_RS03605 (CHBNIV1_06820) ampD 716053..716616 (-) 564 WP_005660014.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J73_RS03610 (CHBNIV1_06830) pilA 716731..717180 (+) 450 WP_005660016.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J73_RS03615 (CHBNIV1_06840) pilB 717177..718574 (+) 1398 WP_005660019.1 GspE/PulE family protein Machinery gene
  K6J73_RS03620 (CHBNIV1_06850) pilC 718571..719791 (+) 1221 WP_005660021.1 type II secretion system F family protein Machinery gene
  K6J73_RS03625 (CHBNIV1_06860) pilD 719788..720480 (+) 693 WP_221253448.1 A24 family peptidase Machinery gene
  K6J73_RS03630 (CHBNIV1_06870) rho 720534..721796 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J73_RS03635 (CHBNIV1_06880) metJ 722043..722360 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J73_RS03640 (CHBNIV1_06890) cueR 722374..722760 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  K6J73_RS03645 (CHBNIV1_06900) - 722837..723043 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  K6J73_RS03650 (CHBNIV1_06910) - 723118..723324 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 52912.27 Da        Isoelectric Point: 5.8432

>NTDB_id=71288 K6J73_RS03615 WP_005660019.1 717177..718574(+) (pilB) [Haemophilus influenzae strain CHBN-IV-1 isolate 13H36]
MTSYALLHTQRVTAQNGEIFTISPDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLAPNQPKVEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGV
LQPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAEL
GMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLL
RAFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSK
CGGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=71288 K6J73_RS03615 WP_005660019.1 717177..718574(+) (pilB) [Haemophilus influenzae strain CHBN-IV-1 isolate 13H36]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACTATCTCGCCAGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTACTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTATTACAGCAACTTGCTCCTAATCAACCTAAAGTGGAAGAGCAAGTTAAATATTA
CCAACATCAAGAACAGTCTCATCTTGAACAACAAGATGATGAACCTGTTATCCGTTTACTTAATCAGATTTTTGAATCTG
CCTTACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTT
TTACAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATAT
TAGTGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAA
CCTTACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTG
GGTATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCAC
AGGAAGTGGGAAAAGTATCTCACTTTACACTGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACAGCGGAAG
ATCCCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTG
CGTGCTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGCGATGAAGAAAGTGCAAGGATTGCATTACG
TGCCGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAAC
TCGGTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGTTCAAAG
TGCGGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTG
GCAACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATG
AGAAAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.989

100

0.97
  pilB Haemophilus influenzae Rd KW20

95.474

99.785

0.953
  pilB Glaesserella parasuis strain SC1401

59.211

98.065

0.581
  pilB Vibrio cholerae strain A1552

41.366

100

0.469
  pilB Vibrio campbellii strain DS40M4

40.695

100

0.428
  pilB Vibrio parahaemolyticus RIMD 2210633

40.58

100

0.422
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.415
  pilB Acinetobacter baylyi ADP1

38.229

100

0.409
  pilB Acinetobacter baumannii D1279779

38.669

100

0.4
  pilF Neisseria gonorrhoeae MS11

37.605

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.74

100

0.381
  pilF Thermus thermophilus HB27

35.876

100

0.374

Multiple sequence alignment