Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J60_RS03840 Genome accession   NZ_AP018778
Coordinates   744673..746067 (+) Length   464 a.a.
NCBI ID   WP_221267468.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-7 isolate 15H01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 739673..751067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J60_RS03815 (CHBNIII7_07230) rsmE 739687..740424 (-) 738 WP_221267466.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J60_RS03820 (CHBNIII7_07240) lnt 740474..742003 (-) 1530 WP_343213440.1 apolipoprotein N-acyltransferase -
  K6J60_RS03825 (CHBNIII7_07250) corC 742026..742925 (-) 900 WP_005662987.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J60_RS03830 (CHBNIII7_07260) ampD 743558..744112 (-) 555 WP_112074623.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J60_RS03835 (CHBNIII7_07270) pilA 744227..744676 (+) 450 WP_112074624.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J60_RS03840 (CHBNIII7_07280) pilB 744673..746067 (+) 1395 WP_221267468.1 GspE/PulE family protein Machinery gene
  K6J60_RS03845 (CHBNIII7_07290) pilC 746064..747281 (+) 1218 WP_112074625.1 type II secretion system F family protein Machinery gene
  K6J60_RS03850 (CHBNIII7_07300) pilD 747278..747970 (+) 693 WP_118787909.1 A24 family peptidase Machinery gene
  K6J60_RS03855 (CHBNIII7_07310) rho 748024..749286 (-) 1263 WP_005629550.1 transcription termination factor Rho -
  K6J60_RS03860 (CHBNIII7_07320) metJ 749534..749851 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J60_RS03865 (CHBNIII7_07330) cueR 749865..750251 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J60_RS03870 (CHBNIII7_07340) - 750328..750534 (+) 207 WP_048941374.1 heavy-metal-associated domain-containing protein -
  K6J60_RS03875 (CHBNIII7_07350) - 750620..750826 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52987.44 Da        Isoelectric Point: 5.7877

>NTDB_id=71217 K6J60_RS03840 WP_221267468.1 744673..746067(+) (pilB) [Haemophilus influenzae strain CHBN-III-7 isolate 15H01]
MTSYALLHTQRVIAQNGEVFTISPDLWERNQQQQSLLLRYFALLLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQENDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTESQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFNRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDVISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=71217 K6J60_RS03840 WP_221267468.1 744673..746067(+) (pilB) [Haemophilus influenzae strain CHBN-III-7 isolate 15H01]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAACAACAATCCTTGCTCTTACGTTATTTTGCTTTGCTACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGTCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAAATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGC
ATGACTGAAAGCCAACAACAGGCATTTCAACACGCTCTTAGCCAACCCCAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACTGCACTTCAATGGCTTAATACACCTGATAAACACATTATGACAGCGGAAGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCGCAGATTGGACTAGATTTTAACCGCCTATTACGC
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGTAATATCTGCCATTTCCCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

97.845

100

0.978
  pilB Haemophilus influenzae Rd KW20

95.68

99.784

0.955
  pilB Glaesserella parasuis strain SC1401

57.112

98.491

0.563
  pilB Vibrio cholerae strain A1552

40.574

100

0.427
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.752

100

0.414
  pilB Legionella pneumophila strain ERS1305867

38.855

100

0.409
  pilB Acinetobacter baylyi ADP1

37.903

100

0.405
  pilB Acinetobacter baumannii D1279779

38.254

100

0.397
  pilF Neisseria gonorrhoeae MS11

36.555

100

0.375
  pilF Thermus thermophilus HB27

36.421

100

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.752

100

0.371

Multiple sequence alignment