Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J60_RS03835 Genome accession   NZ_AP018778
Coordinates   744227..744676 (+) Length   149 a.a.
NCBI ID   WP_112074624.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-7 isolate 15H01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 739227..749676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J60_RS03815 (CHBNIII7_07230) rsmE 739687..740424 (-) 738 WP_221267466.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J60_RS03820 (CHBNIII7_07240) lnt 740474..742003 (-) 1530 WP_343213440.1 apolipoprotein N-acyltransferase -
  K6J60_RS03825 (CHBNIII7_07250) corC 742026..742925 (-) 900 WP_005662987.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J60_RS03830 (CHBNIII7_07260) ampD 743558..744112 (-) 555 WP_112074623.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J60_RS03835 (CHBNIII7_07270) pilA 744227..744676 (+) 450 WP_112074624.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J60_RS03840 (CHBNIII7_07280) pilB 744673..746067 (+) 1395 WP_221267468.1 GspE/PulE family protein Machinery gene
  K6J60_RS03845 (CHBNIII7_07290) pilC 746064..747281 (+) 1218 WP_112074625.1 type II secretion system F family protein Machinery gene
  K6J60_RS03850 (CHBNIII7_07300) pilD 747278..747970 (+) 693 WP_118787909.1 A24 family peptidase Machinery gene
  K6J60_RS03855 (CHBNIII7_07310) rho 748024..749286 (-) 1263 WP_005629550.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15452.82 Da        Isoelectric Point: 8.7927

>NTDB_id=71216 K6J60_RS03835 WP_112074624.1 744227..744676(+) (pilA) [Haemophilus influenzae strain CHBN-III-7 isolate 15H01]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSTCSGGNNGIA
ADIKTAKGYVASVKTQSGGITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=71216 K6J60_RS03835 WP_112074624.1 744227..744676(+) (pilA) [Haemophilus influenzae strain CHBN-III-7 isolate 15H01]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGTTTTACATTAATCGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCGTCTGCGC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGCAAACCTTCAACTTGCTCAGGAGGAAACAATGGAATTGCA
GCGGATATAAAGACAGCAAAAGGCTATGTAGCCTCAGTGAAAACTCAATCGGGTGGCATTACCGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

91.275

100

0.913
  pilA Haemophilus influenzae Rd KW20

91.275

100

0.913
  pilA Glaesserella parasuis strain SC1401

56.376

100

0.564
  pilA2 Legionella pneumophila str. Paris

39.189

99.329

0.389
  pilA2 Legionella pneumophila strain ERS1305867

39.189

99.329

0.389
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376

Multiple sequence alignment