Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K6J60_RS03850 Genome accession   NZ_AP018778
Coordinates   747278..747970 (+) Length   230 a.a.
NCBI ID   WP_118787909.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-7 isolate 15H01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742278..752970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J60_RS03830 (CHBNIII7_07260) ampD 743558..744112 (-) 555 WP_112074623.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J60_RS03835 (CHBNIII7_07270) pilA 744227..744676 (+) 450 WP_112074624.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J60_RS03840 (CHBNIII7_07280) pilB 744673..746067 (+) 1395 WP_221267468.1 GspE/PulE family protein Machinery gene
  K6J60_RS03845 (CHBNIII7_07290) pilC 746064..747281 (+) 1218 WP_112074625.1 type II secretion system F family protein Machinery gene
  K6J60_RS03850 (CHBNIII7_07300) pilD 747278..747970 (+) 693 WP_118787909.1 A24 family peptidase Machinery gene
  K6J60_RS03855 (CHBNIII7_07310) rho 748024..749286 (-) 1263 WP_005629550.1 transcription termination factor Rho -
  K6J60_RS03860 (CHBNIII7_07320) metJ 749534..749851 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J60_RS03865 (CHBNIII7_07330) cueR 749865..750251 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J60_RS03870 (CHBNIII7_07340) - 750328..750534 (+) 207 WP_048941374.1 heavy-metal-associated domain-containing protein -
  K6J60_RS03875 (CHBNIII7_07350) - 750620..750826 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  K6J60_RS03880 (CHBNIII7_07360) - 750912..751118 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26660.63 Da        Isoelectric Point: 8.8544

>NTDB_id=71219 K6J60_RS03850 WP_118787909.1 747278..747970(+) (pilD) [Haemophilus influenzae strain CHBN-III-7 isolate 15H01]
MIYFTMFLLGGIFGIALWFYLSGFITRLQQEIYATYIELFPQNFLPFQSSVSSIQQKKCGHILRYFLSVGVGFIFLQIAF
KDSIFTVWFGLTLLILWAISYLDWHYQLISTTPCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYTIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYLVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=71219 K6J60_RS03850 WP_118787909.1 747278..747970(+) (pilD) [Haemophilus influenzae strain CHBN-III-7 isolate 15H01]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTCGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACCCG
TTTACAGCAAGAGATTTATGCGACTTATATTGAATTATTCCCACAAAACTTTCTGCCATTTCAGTCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAGTGTTGGGGTAGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGTTCGGACTCACACTTCTTATCCTTTGGGCAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACACCCTGTTTATGGCTACTTACAATCGGTTTATTTGGCGCAGACAATAACTTTTCATTGCTTACGT
TATCTGAAAGTATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACACAATCTATTGGATTGCAAAATTCTATTACGGC
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTGCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCGGCTGTCATTATTTATCTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

91.739

100

0.917
  pilD Haemophilus influenzae Rd KW20

90.87

100

0.909

Multiple sequence alignment