Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J67_RS03600 Genome accession   NZ_AP018776
Coordinates   707864..709261 (+) Length   465 a.a.
NCBI ID   WP_042601843.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-5 isolate 13H30     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 702864..714261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J67_RS03575 (CHBNIII5_06880) rsmE 702882..703619 (-) 738 WP_050822717.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J67_RS03580 (CHBNIII5_06890) lnt 703669..705198 (-) 1530 WP_080358502.1 apolipoprotein N-acyltransferase -
  K6J67_RS03585 (CHBNIII5_06900) corC 705221..706120 (-) 900 WP_011961742.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J67_RS03590 (CHBNIII5_06910) ampD 706743..707303 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J67_RS03595 (CHBNIII5_06920) pilA 707418..707867 (+) 450 WP_042601842.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J67_RS03600 (CHBNIII5_06930) pilB 707864..709261 (+) 1398 WP_042601843.1 GspE/PulE family protein Machinery gene
  K6J67_RS03605 (CHBNIII5_06940) pilC 709258..710478 (+) 1221 WP_042601844.1 type II secretion system F family protein Machinery gene
  K6J67_RS03610 (CHBNIII5_06950) pilD 710475..711167 (+) 693 WP_050822711.1 A24 family peptidase Machinery gene
  K6J67_RS03615 (CHBNIII5_06960) rho 711221..712483 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  K6J67_RS03620 (CHBNIII5_06970) metJ 712731..713048 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J67_RS03625 (CHBNIII5_06980) cueR 713062..713448 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J67_RS03630 (CHBNIII5_06990) - 713525..713731 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  K6J67_RS03635 (CHBNIII5_07000) - 713805..714011 (+) 207 WP_012054944.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 52992.39 Da        Isoelectric Point: 5.8115

>NTDB_id=71149 K6J67_RS03600 WP_042601843.1 707864..709261(+) (pilB) [Haemophilus influenzae strain CHBN-III-5 isolate 13H30]
MTSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGV
LQPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAEL
GMTESQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNMPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLL
RAFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSK
CGGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=71149 K6J67_RS03600 WP_042601843.1 707864..709261(+) (pilB) [Haemophilus influenzae strain CHBN-III-5 isolate 13H30]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGTCTGATTTATG
GGCGCGTAATCAACAACAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAGCAAGTTAAATATTA
CCAACATCAAGAACAGTCTCATCTTGAACAACAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTG
CCTTACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTT
TTACAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATAT
TAGTGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAA
CCTTACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTG
GGCATGACTGAAAGCCAACAACAGGCATTTCAACACGCGCTTAGCCAACCCCAAGGATTAATTTTAGTCACTGGCCCAAC
AGGAAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATATGCCTGATAAACATATTATGACTGCTGAGG
ATCCCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTG
CGCGCCTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCAAGGATTGCATTACG
TGCCGCCCAAACAGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAAC
TCGGTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGTTCAAAG
TGCGGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTG
GCAACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATG
AGAAAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.774

100

0.968
  pilB Haemophilus influenzae Rd KW20

95.043

99.785

0.948
  pilB Glaesserella parasuis strain SC1401

58.553

98.065

0.574
  pilB Vibrio cholerae strain A1552

40.607

100

0.46
  pilB Vibrio campbellii strain DS40M4

41.104

100

0.432
  pilB Vibrio parahaemolyticus RIMD 2210633

40.787

100

0.424
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.417
  pilB Acinetobacter baylyi ADP1

37.98

100

0.404
  pilB Acinetobacter baumannii D1279779

38.462

100

0.398
  pilF Neisseria gonorrhoeae MS11

37.605

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.74

100

0.381
  pilF Thermus thermophilus HB27

35.789

100

0.366

Multiple sequence alignment