Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   M2043_RS21555 Genome accession   NZ_AP023433
Coordinates   4446481..4447158 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain M719     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4441481..4452158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2043_RS21520 (MYEC719_4367) gspG 4441961..4442398 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  M2043_RS21525 (MYEC719_4368) gspH 4442406..4442915 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  M2043_RS21530 (MYEC719_4369) gspI 4442912..4443289 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  M2043_RS21535 (MYEC719_4370) gspJ 4443282..4443869 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  M2043_RS21540 (MYEC719_4371) gspK 4443862..4444845 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  M2043_RS21545 (MYEC719_4372) gspL 4444860..4446023 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  M2043_RS21550 (MYEC719_4373) gspM 4446020..4446481 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  M2043_RS21555 (MYEC719_4374) pilD 4446481..4447158 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  M2043_RS21560 (MYEC719_4375) bfr 4447187..4447663 (-) 477 WP_000675504.1 bacterioferritin -
  M2043_RS21565 (MYEC719_4376) bfd 4447735..4447929 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  M2043_RS21570 (MYEC719_4378) chiA 4448098..4450791 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=71150 M2043_RS21555 WP_000178161.1 4446481..4447158(+) (pilD) [Escherichia coli strain M719]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=71150 M2043_RS21555 WP_000178161.1 4446481..4447158(+) (pilD) [Escherichia coli strain M719]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment