Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J67_RS03595 Genome accession   NZ_AP018776
Coordinates   707418..707867 (+) Length   149 a.a.
NCBI ID   WP_042601842.1    Uniprot ID   Q5D8C1
Organism   Haemophilus influenzae strain CHBN-III-5 isolate 13H30     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 702418..712867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J67_RS03575 (CHBNIII5_06880) rsmE 702882..703619 (-) 738 WP_050822717.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J67_RS03580 (CHBNIII5_06890) lnt 703669..705198 (-) 1530 WP_080358502.1 apolipoprotein N-acyltransferase -
  K6J67_RS03585 (CHBNIII5_06900) corC 705221..706120 (-) 900 WP_011961742.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J67_RS03590 (CHBNIII5_06910) ampD 706743..707303 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J67_RS03595 (CHBNIII5_06920) pilA 707418..707867 (+) 450 WP_042601842.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J67_RS03600 (CHBNIII5_06930) pilB 707864..709261 (+) 1398 WP_042601843.1 GspE/PulE family protein Machinery gene
  K6J67_RS03605 (CHBNIII5_06940) pilC 709258..710478 (+) 1221 WP_042601844.1 type II secretion system F family protein Machinery gene
  K6J67_RS03610 (CHBNIII5_06950) pilD 710475..711167 (+) 693 WP_050822711.1 A24 family peptidase Machinery gene
  K6J67_RS03615 (CHBNIII5_06960) rho 711221..712483 (-) 1263 WP_005666690.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15400.70 Da        Isoelectric Point: 8.4623

>NTDB_id=71148 K6J67_RS03595 WP_042601842.1 707418..707867(+) (pilA) [Haemophilus influenzae strain CHBN-III-5 isolate 13H30]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSSCSGGSNGIA
ADITTAKGYVKSVTTSNGAITVKGDGTLANMEYILQASGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=71148 K6J67_RS03595 WP_042601842.1 707418..707867(+) (pilA) [Haemophilus influenzae strain CHBN-III-5 isolate 13H30]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCGGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGTACAGGTAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGAATTGCG
GCTGATATTACAACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCCAGTGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5D8C1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

94.631

100

0.946
  pilA Haemophilus influenzae Rd KW20

88.591

100

0.886
  pilA Glaesserella parasuis strain SC1401

56.376

100

0.564
  pilA2 Legionella pneumophila str. Paris

39.189

99.329

0.389
  pilA2 Legionella pneumophila strain ERS1305867

39.189

99.329

0.389
  comP Acinetobacter baylyi ADP1

42.52

85.235

0.362
  pilA Vibrio campbellii strain DS40M4

38.298

94.631

0.362

Multiple sequence alignment