Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   NN994_RS08660 Genome accession   NZ_CP101646
Coordinates   1660302..1661243 (-) Length   313 a.a.
NCBI ID   WP_014608745.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain UCCSt95     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1655302..1666243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NN994_RS08620 (NN994_08625) - 1655378..1656571 (-) 1194 WP_014608740.1 acetate kinase -
  NN994_RS08625 (NN994_08630) comYH 1656627..1657583 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  NN994_RS08630 (NN994_08635) comGG 1657628..1657945 (-) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  NN994_RS08635 (NN994_08640) comYF 1657923..1658360 (-) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  NN994_RS08640 (NN994_08645) comGE 1658344..1658637 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  NN994_RS08645 (NN994_08650) comYD 1658609..1659037 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  NN994_RS08650 (NN994_08655) comYC 1658997..1659323 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  NN994_RS08655 (NN994_08660) comYB 1659320..1660420 (-) 1101 WP_120764773.1 competence type IV pilus assembly protein ComGB Machinery gene
  NN994_RS08660 (NN994_08665) comGA/cglA/cilD 1660302..1661243 (-) 942 WP_014608745.1 competence type IV pilus ATPase ComGA Machinery gene
  NN994_RS08665 (NN994_08670) - 1661324..1661686 (-) 363 WP_014608746.1 DUF1033 family protein -
  NN994_RS08670 (NN994_08675) rpoC 1661845..1665483 (-) 3639 WP_011681690.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35508.70 Da        Isoelectric Point: 6.6091

>NTDB_id=711243 NN994_RS08660 WP_014608745.1 1660302..1661243(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain UCCSt95]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVARMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=711243 NN994_RS08660 WP_014608745.1 1660302..1661243(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain UCCSt95]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGAGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCTGAACGTCGGGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGGGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.273

99.361

0.649
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.987

99.361

0.636
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.218

99.681

0.61
  comGA Lactococcus lactis subsp. cremoris KW2

50.804

99.361

0.505
  comGA Latilactobacillus sakei subsp. sakei 23K

38.487

97.125

0.374