Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NNL22_RS10370 Genome accession   NZ_CP101527
Coordinates   2333051..2334271 (+) Length   406 a.a.
NCBI ID   WP_251809592.1    Uniprot ID   A0A9E8HNB6
Organism   Alkalimarinus sediminis strain FA028     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2328051..2339271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNL22_RS10345 (NNL22_10335) - 2328524..2328694 (-) 171 WP_251809589.1 hypothetical protein -
  NNL22_RS10350 (NNL22_10340) - 2329051..2330466 (-) 1416 WP_251809590.1 O-antigen ligase family protein -
  NNL22_RS10355 (NNL22_10345) - 2330518..2330994 (-) 477 WP_275116306.1 pilin -
  NNL22_RS10365 (NNL22_10355) pilB 2331339..2333048 (+) 1710 WP_251809591.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NNL22_RS10370 (NNL22_10360) pilC 2333051..2334271 (+) 1221 WP_251809592.1 type II secretion system F family protein Machinery gene
  NNL22_RS10375 (NNL22_10365) pilD 2334339..2335208 (+) 870 WP_251809593.1 prepilin peptidase Machinery gene
  NNL22_RS10380 (NNL22_10370) coaE 2335215..2335802 (+) 588 WP_251809594.1 dephospho-CoA kinase -
  NNL22_RS10385 (NNL22_10375) yacG 2335892..2336119 (+) 228 WP_251809595.1 DNA gyrase inhibitor YacG -
  NNL22_RS10390 (NNL22_10380) - 2336168..2336614 (+) 447 WP_251809596.1 HIT family protein -
  NNL22_RS10395 (NNL22_10385) - 2336630..2336989 (+) 360 WP_251809597.1 CidA/LrgA family protein -
  NNL22_RS10400 (NNL22_10390) - 2336986..2337705 (+) 720 WP_251809598.1 LrgB family protein -
  NNL22_RS10405 (NNL22_10395) tsaA 2337717..2338430 (+) 714 WP_251809599.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  NNL22_RS10410 (NNL22_10400) - 2338555..2338992 (-) 438 WP_251809600.1 PilZ domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44341.94 Da        Isoelectric Point: 7.8133

>NTDB_id=710404 NNL22_RS10370 WP_251809592.1 2333051..2334271(+) (pilC) [Alkalimarinus sediminis strain FA028]
MVEKAEKLSAYNWEGTDRKGNKTKGKTNGKNAALVKAQLRQQGITPIKVKKEAISLGGPKKKKITPFDIAIFTRQLATMM
KAGVPLIQAFDIVAEGLENPSLRDVVLDIKTEVAAGNNFAGALRKHPKYFDDLFCNLVDSGEQSGALETMLDRVATYMEK
TETLKKKVKKAMSYPIAVVCVAVIVTAILLIKVVPQFEELFSGFGAELPAFTQFVIGISEWMQEWWYVGLGIIIATIFIF
KEAKQRSSAFADKLDHLILRLPVVGIIIDKSAIARFGRVLSTTFAAGVPLVDALDSVAGAAGNAKYRDAIYRIRDDVSTG
TQLQFSMKQTNVFPAMAVQLVSIGEESGALDSMLEKVADYYEEEVDALVDSLTSLMEPIIMSVLGVLVGGLIVAMYLPIF
QMGQVV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=710404 NNL22_RS10370 WP_251809592.1 2333051..2334271(+) (pilC) [Alkalimarinus sediminis strain FA028]
ATGGTTGAAAAAGCAGAAAAATTAAGTGCCTATAATTGGGAAGGAACTGACCGAAAAGGTAACAAGACCAAGGGTAAAAC
AAACGGTAAAAATGCTGCGTTAGTAAAAGCACAGCTCCGGCAACAAGGCATAACTCCAATTAAAGTAAAAAAAGAAGCAA
TATCACTCGGAGGTCCCAAGAAAAAGAAGATCACCCCATTTGATATTGCTATATTTACCCGACAACTCGCTACAATGATG
AAGGCAGGCGTGCCACTGATCCAAGCATTTGATATTGTTGCAGAAGGCCTGGAAAACCCAAGCCTTCGCGATGTTGTACT
AGACATTAAAACCGAAGTCGCCGCTGGTAACAACTTCGCTGGCGCTCTTAGAAAACACCCAAAGTACTTCGACGATTTAT
TTTGTAATCTAGTAGACTCTGGTGAACAGTCCGGTGCTTTAGAAACCATGCTTGATCGTGTTGCGACATACATGGAGAAA
ACAGAAACACTTAAGAAAAAAGTTAAAAAAGCAATGAGCTACCCAATCGCTGTAGTATGTGTTGCGGTGATCGTAACAGC
AATTCTACTTATTAAAGTAGTTCCCCAGTTTGAAGAACTTTTTTCTGGATTTGGAGCGGAGCTTCCAGCATTTACTCAGT
TTGTAATTGGAATTTCCGAGTGGATGCAAGAATGGTGGTATGTCGGGCTTGGTATAATTATCGCCACTATTTTCATATTT
AAAGAAGCTAAACAAAGATCTAGTGCATTTGCAGATAAACTTGACCACCTCATTCTGCGATTACCAGTTGTTGGAATCAT
AATAGATAAGTCTGCAATAGCAAGATTTGGGCGAGTATTATCAACAACTTTTGCAGCAGGCGTACCATTAGTCGATGCCC
TAGACTCTGTGGCTGGTGCAGCTGGAAATGCCAAATATCGCGATGCAATTTATAGAATAAGAGATGATGTTTCCACCGGT
ACTCAACTGCAGTTTTCAATGAAACAAACGAATGTATTTCCTGCCATGGCTGTTCAACTTGTTTCTATTGGCGAAGAATC
TGGTGCGCTAGACTCTATGCTTGAAAAAGTAGCTGACTATTATGAAGAAGAGGTTGACGCCCTTGTCGACAGCCTGACTA
GTTTGATGGAACCGATAATAATGTCTGTGTTGGGTGTGCTAGTTGGCGGCTTAATTGTTGCCATGTACTTACCGATATTC
CAGATGGGGCAAGTTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(278-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

66.995

100

0.67
  pilC Acinetobacter baumannii D1279779

62.408

100

0.626
  pilC Acinetobacter baylyi ADP1

61.823

100

0.618
  pilC Legionella pneumophila strain ERS1305867

54.926

100

0.549
  pilG Neisseria gonorrhoeae MS11

44.089

100

0.441
  pilG Neisseria meningitidis 44/76-A

44.089

100

0.441
  pilC Vibrio campbellii strain DS40M4

41.191

99.261

0.409
  pilC Vibrio cholerae strain A1552

41.103

98.276

0.404
  pilC Thermus thermophilus HB27

36.946

100

0.369