Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NNL22_RS10365 Genome accession   NZ_CP101527
Coordinates   2331339..2333048 (+) Length   569 a.a.
NCBI ID   WP_251809591.1    Uniprot ID   A0A9E8HFU4
Organism   Alkalimarinus sediminis strain FA028     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2314804..2330490 2331339..2333048 flank 849


Gene organization within MGE regions


Location: 2314804..2333048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNL22_RS10285 (NNL22_10275) - 2314804..2315877 (-) 1074 WP_251809577.1 Wzz/FepE/Etk N-terminal domain-containing protein -
  NNL22_RS10290 (NNL22_10280) - 2315940..2317451 (-) 1512 WP_251809578.1 hypothetical protein -
  NNL22_RS10295 (NNL22_10285) rfbD 2317663..2318556 (-) 894 WP_251809579.1 dTDP-4-dehydrorhamnose reductase -
  NNL22_RS10300 (NNL22_10290) rfbC 2318568..2319110 (-) 543 WP_251809580.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  NNL22_RS10305 (NNL22_10295) rfbA 2319119..2319994 (-) 876 WP_251809581.1 glucose-1-phosphate thymidylyltransferase RfbA -
  NNL22_RS10310 (NNL22_10300) rfbB 2319997..2321073 (-) 1077 WP_251809582.1 dTDP-glucose 4,6-dehydratase -
  NNL22_RS10315 (NNL22_10305) - 2321114..2322139 (-) 1026 WP_251809583.1 NAD-dependent epimerase/dehydratase family protein -
  NNL22_RS10320 (NNL22_10310) - 2322238..2323629 (-) 1392 WP_251809584.1 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  NNL22_RS10325 (NNL22_10315) - 2324006..2324326 (+) 321 WP_251809585.1 ComEA family DNA-binding protein -
  NNL22_RS10330 (NNL22_10320) - 2324469..2325824 (-) 1356 WP_251809586.1 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  NNL22_RS10335 (NNL22_10325) - 2326082..2327611 (+) 1530 WP_251809587.1 thymidine phosphorylase family protein -
  NNL22_RS10340 (NNL22_10330) - 2327608..2328498 (+) 891 WP_251809588.1 ribose-phosphate pyrophosphokinase -
  NNL22_RS10345 (NNL22_10335) - 2328524..2328694 (-) 171 WP_251809589.1 hypothetical protein -
  NNL22_RS10350 (NNL22_10340) - 2329051..2330466 (-) 1416 WP_251809590.1 O-antigen ligase family protein -
  NNL22_RS10355 (NNL22_10345) - 2330518..2330994 (-) 477 WP_275116306.1 pilin -
  NNL22_RS10365 (NNL22_10355) pilB 2331339..2333048 (+) 1710 WP_251809591.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62532.61 Da        Isoelectric Point: 4.8165

>NTDB_id=710403 NNL22_RS10365 WP_251809591.1 2331339..2333048(+) (pilB) [Alkalimarinus sediminis strain FA028]
MASNSAITLTGLARKFVLDNLLEEEVAKDAFTQASNNNIPFITYLVQNKLAGAKALAYSAAQEFGMPVMDISTFLVELIP
EKIVDEKLIRKHHALPLFKRGNRLFLATSDPTNIQALDELKFHTGLSTDAVLVEDDKLSDAIEKYLESQDTSMGDLEGGD
LDDLDIESGETEQEEAQNNEADDAPIVKYVNKMLLDAIKGGASDLHFEPYEKSYRVRYRTDGILHEVSKPSIKLAPKIAA
RIKVMCQMDISERRIPQDGRIKLKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKMGIDALGYEEDQKELYMQALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPETNISTAEDPVEINLEGINQVNVNPKVGLGFPEALRSFLRQDPDIVMVGEIR
DLDTASIAIKAAQTGHLVLSTLHTNSAPETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCNNCKEKADIPKDVLIKQGF
TEEQIETGFTLYQPKGCDKCSGGYKGRVGIYEVVKITPAISQLIMEEATSLDIAAAAQKEGFNNLRQSAMLKAIEGVTSL
EEVNRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=710403 NNL22_RS10365 WP_251809591.1 2331339..2333048(+) (pilB) [Alkalimarinus sediminis strain FA028]
ATGGCATCTAATAGCGCAATCACACTCACAGGTTTAGCCCGAAAGTTTGTGTTGGACAACCTTCTTGAAGAAGAGGTTGC
GAAAGACGCATTTACTCAGGCTAGCAACAACAATATTCCATTTATTACCTACCTGGTACAAAACAAGCTAGCAGGCGCCA
AAGCTTTAGCCTACTCTGCAGCACAAGAGTTCGGGATGCCTGTAATGGATATTTCAACTTTTCTGGTTGAGTTAATCCCT
GAAAAGATTGTTGATGAGAAGCTTATTAGAAAGCATCATGCGCTTCCCCTCTTTAAAAGAGGTAACCGGCTGTTTTTAGC
GACCTCTGACCCAACCAATATTCAAGCGCTCGATGAACTCAAGTTTCACACAGGGTTAAGTACAGACGCCGTTCTGGTTG
AAGACGATAAGCTCAGTGACGCTATTGAGAAATACCTAGAGTCGCAAGATACTTCGATGGGAGATCTCGAGGGCGGAGAC
TTAGATGATCTAGACATTGAATCGGGTGAGACAGAGCAAGAGGAAGCACAAAACAATGAGGCAGACGATGCACCTATTGT
AAAATACGTCAACAAAATGCTATTGGATGCTATAAAAGGCGGCGCCTCAGATTTACATTTCGAACCATACGAGAAATCAT
ACCGAGTTCGATACCGAACGGATGGTATTTTGCATGAAGTATCTAAACCTTCAATTAAGCTTGCGCCCAAAATTGCTGCC
CGCATAAAAGTAATGTGCCAAATGGACATTTCTGAAAGGCGCATCCCTCAAGACGGACGGATAAAGTTAAAACTCTCTAA
AACAAAAGCCATTGATTTTCGAGTCAACACCCTCCCTACACTATGGGGAGAGAAAATCGTACTACGTATCCTTGACCCTA
GCAGTGCCAAAATGGGGATTGACGCACTCGGCTATGAAGAGGATCAAAAAGAACTTTATATGCAAGCCTTGGCACAACCA
CAAGGTATGATTTTAGTAACAGGCCCTACAGGTAGCGGTAAGACAGTATCACTTTATACAGGATTGAATATTCTTAATAC
GCCAGAAACCAATATATCAACGGCTGAAGACCCAGTAGAAATCAACCTAGAGGGTATAAACCAGGTTAACGTTAACCCTA
AGGTTGGCTTAGGTTTCCCAGAAGCACTGAGATCATTCTTAAGGCAAGACCCTGATATTGTTATGGTGGGAGAGATACGG
GACTTAGATACTGCATCTATCGCAATCAAAGCCGCTCAAACAGGTCACTTGGTATTGTCAACACTGCACACCAATAGTGC
CCCTGAAACCTTAACCCGTATGATGAATATGGGCGTACCTGCTTTTAATATCGCGACATCTGTCAGCTTAATCATCGCTC
AGCGATTGGGCAGACGCTTATGCAATAACTGCAAAGAGAAAGCAGATATTCCAAAAGATGTACTTATTAAGCAAGGGTTC
ACAGAAGAACAAATTGAAACAGGGTTTACATTGTACCAACCAAAAGGCTGTGATAAATGTTCAGGTGGATACAAAGGGCG
TGTCGGTATTTATGAAGTGGTTAAAATTACACCTGCTATATCTCAATTGATAATGGAAGAAGCGACATCGCTTGATATAG
CAGCAGCAGCCCAAAAAGAAGGTTTTAATAATTTAAGACAGTCAGCAATGCTAAAAGCAATTGAAGGCGTGACAAGCTTG
GAAGAAGTTAACCGAGTGACTAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.825

100

0.599
  pilB Acinetobacter baylyi ADP1

57.473

98.77

0.568
  pilB Legionella pneumophila strain ERS1305867

56.89

99.473

0.566
  pilB Vibrio cholerae strain A1552

51.292

95.255

0.489
  pilF Neisseria gonorrhoeae MS11

48.319

99.297

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

50.558

94.552

0.478
  pilB Vibrio campbellii strain DS40M4

52.86

89.104

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.462

91.213

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

40.154

91.037

0.366