Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J66_RS03565 Genome accession   NZ_AP018771
Coordinates   708595..709992 (+) Length   465 a.a.
NCBI ID   WP_038439229.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-8 isolate 10H27     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 703595..714992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J66_RS03540 (CHBNII8_06740) rsmE 703622..704359 (-) 738 WP_038439236.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J66_RS03545 (CHBNII8_06750) lnt 704409..705938 (-) 1530 WP_080281417.1 apolipoprotein N-acyltransferase -
  K6J66_RS03550 (CHBNII8_06760) corC 705961..706860 (-) 900 WP_038439233.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J66_RS03555 (CHBNII8_06770) ampD 707483..708034 (-) 552 WP_038439232.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J66_RS03560 (CHBNII8_06780) pilA 708149..708598 (+) 450 WP_038439231.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J66_RS03565 (CHBNII8_06790) pilB 708595..709992 (+) 1398 WP_038439229.1 GspE/PulE family protein Machinery gene
  K6J66_RS03570 (CHBNII8_06800) pilC 709989..711209 (+) 1221 WP_038439228.1 type II secretion system F family protein Machinery gene
  K6J66_RS03575 (CHBNII8_06810) pilD 711206..711898 (+) 693 WP_038439227.1 A24 family peptidase Machinery gene
  K6J66_RS03580 (CHBNII8_06820) rho 711953..713215 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  K6J66_RS03585 (CHBNII8_06830) metJ 713463..713780 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J66_RS03590 (CHBNII8_06840) cueR 713794..714180 (-) 387 WP_038439225.1 Cu(I)-responsive transcriptional regulator -
  K6J66_RS03595 (CHBNII8_06850) - 714257..714463 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  K6J66_RS03600 (CHBNII8_06860) - 714537..714743 (+) 207 WP_005686717.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53073.40 Da        Isoelectric Point: 5.6399

>NTDB_id=70971 K6J66_RS03565 WP_038439229.1 708595..709992(+) (pilB) [Haemophilus influenzae strain CHBN-II-8 isolate 10H27]
MTSYALLHTQRVTAQNGEIFTISPDLWERNQQQQSLLLRYFALPIKEENNRLWLGVDSLSNLSACETISFITGKPVEPIL
LESSQLKELLQQLTPHQMQVEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGV
LQPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAEL
GMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLL
RAFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSK
CGGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=70971 K6J66_RS03565 WP_038439229.1 708595..709992(+) (pilB) [Haemophilus influenzae strain CHBN-II-8 isolate 10H27]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCAATTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTTCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGTGGAAGAGCAAGTTAAATATTA
CCAACATCAAGAACAGTCTCATCTTGAACAACAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTG
CCTTACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTT
TTACAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATAT
TAGTGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAA
CCTTACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTG
GGTATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGCCCAAC
AGGAAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAATGGCTTAATACACCTGATAAACATATTATGACAGCGGAAG
ATCCCATTGAAATTGAGCTTGATGGTATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTG
CGCGCTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGCGATGAAGAAAGTGCAAGGATTGCATTACG
TGCCGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAAC
TCGGTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGTTCAAAG
TGCGGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTG
GCAACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATG
AGAAAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

97.634

100

0.976
  pilB Haemophilus influenzae Rd KW20

95.69

99.785

0.955
  pilB Glaesserella parasuis strain SC1401

58.772

98.065

0.576
  pilB Vibrio cholerae strain A1552

40.607

100

0.46
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.419
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.417
  pilB Acinetobacter baylyi ADP1

38.028

100

0.406
  pilB Acinetobacter baumannii D1279779

38.462

100

0.398
  pilF Neisseria gonorrhoeae MS11

37.605

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.74

100

0.381
  pilF Thermus thermophilus HB27

36

100

0.368

Multiple sequence alignment