Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J66_RS03560 Genome accession   NZ_AP018771
Coordinates   708149..708598 (+) Length   149 a.a.
NCBI ID   WP_038439231.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-8 isolate 10H27     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 703149..713598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J66_RS03540 (CHBNII8_06740) rsmE 703622..704359 (-) 738 WP_038439236.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J66_RS03545 (CHBNII8_06750) lnt 704409..705938 (-) 1530 WP_080281417.1 apolipoprotein N-acyltransferase -
  K6J66_RS03550 (CHBNII8_06760) corC 705961..706860 (-) 900 WP_038439233.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J66_RS03555 (CHBNII8_06770) ampD 707483..708034 (-) 552 WP_038439232.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J66_RS03560 (CHBNII8_06780) pilA 708149..708598 (+) 450 WP_038439231.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J66_RS03565 (CHBNII8_06790) pilB 708595..709992 (+) 1398 WP_038439229.1 GspE/PulE family protein Machinery gene
  K6J66_RS03570 (CHBNII8_06800) pilC 709989..711209 (+) 1221 WP_038439228.1 type II secretion system F family protein Machinery gene
  K6J66_RS03575 (CHBNII8_06810) pilD 711206..711898 (+) 693 WP_038439227.1 A24 family peptidase Machinery gene
  K6J66_RS03580 (CHBNII8_06820) rho 711953..713215 (-) 1263 WP_005666690.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15565.90 Da        Isoelectric Point: 8.1022

>NTDB_id=70970 K6J66_RS03560 WP_038439231.1 708149..708598(+) (pilA) [Haemophilus influenzae strain CHBN-II-8 isolate 10H27]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQHYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITTAKGYVASVKTQSGGITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=70970 K6J66_RS03560 WP_038439231.1 708149..708598(+) (pilA) [Haemophilus influenzae strain CHBN-II-8 isolate 10H27]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAACATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGTACAAATGAAACAACAAATTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAGCCTCAGTAAAAACGCAATCAGGTGGCATTACCGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

95.973

100

0.96
  pilA Haemophilus influenzae Rd KW20

93.96

100

0.94
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila strain ERS1305867

40.136

98.658

0.396
  pilA Vibrio campbellii strain DS40M4

41.135

94.631

0.389
  pilA2 Legionella pneumophila str. Paris

40.288

93.289

0.376

Multiple sequence alignment