Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NK667_RS32320 Genome accession   NZ_CP101125
Coordinates   7282483..7283700 (-) Length   405 a.a.
NCBI ID   WP_054616651.1    Uniprot ID   -
Organism   Pseudomonas nunensis strain In5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7277483..7288700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NK667_RS32280 (NK667_32280) - 7278288..7278461 (-) 174 WP_054051322.1 DUF3094 family protein -
  NK667_RS32285 (NK667_32285) - 7278516..7279043 (-) 528 WP_054616654.1 MOSC domain-containing protein -
  NK667_RS32290 (NK667_32290) - 7279040..7279666 (-) 627 WP_008154887.1 DUF1780 domain-containing protein -
  NK667_RS32295 (NK667_32295) - 7279801..7280490 (+) 690 WP_054051318.1 energy-coupling factor ABC transporter permease -
  NK667_RS32300 (NK667_32300) - 7280554..7280769 (+) 216 WP_008033212.1 hypothetical protein -
  NK667_RS32305 (NK667_32305) yacG 7280792..7280992 (-) 201 WP_054051315.1 DNA gyrase inhibitor YacG -
  NK667_RS32310 (NK667_32310) coaE 7280989..7281612 (-) 624 WP_054616653.1 dephospho-CoA kinase -
  NK667_RS32315 (NK667_32315) pilD 7281609..7282481 (-) 873 WP_054616652.1 prepilin peptidase Machinery gene
  NK667_RS32320 (NK667_32320) pilC 7282483..7283700 (-) 1218 WP_054616651.1 type II secretion system F family protein Machinery gene
  NK667_RS32325 (NK667_32325) pilB 7283703..7285403 (-) 1701 WP_054616650.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NK667_RS32330 (NK667_32330) - 7285629..7286051 (+) 423 WP_054051305.1 pilin -
  NK667_RS32335 (NK667_32335) - 7286408..7286758 (+) 351 WP_054616649.1 BON domain-containing protein -
  NK667_RS32345 (NK667_32345) - 7287073..7287369 (-) 297 WP_054051301.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44398.32 Da        Isoelectric Point: 9.9725

>NTDB_id=708920 NK667_RS32320 WP_054616651.1 7282483..7283700(-) (pilC) [Pseudomonas nunensis strain In5]
MAVKAAKISVYAWEGTDKKGTKMNGELSGQNPALIKAQLRKQGINPGKVRKKSASIFSFGKRIKAQDISLFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EQLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFKTVFSGFGAELPSFTLMVIDLSEFMQEWWWVMLGALIAAIFGFR
RAMKKSQPFRDRMDTWLLKLPLVGALMYKSAVARYARTLSTTFAAGVPLVDALESVAGATGNIVFKRAVLRIKQDVSTGM
QLNFAMRTSGVFPNMAIQMAAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=708920 NK667_RS32320 WP_054616651.1 7282483..7283700(-) (pilC) [Pseudomonas nunensis strain In5]
ATGGCGGTCAAGGCAGCAAAAATCAGCGTGTACGCCTGGGAAGGCACGGACAAGAAAGGCACGAAAATGAACGGCGAGTT
GAGCGGTCAGAACCCGGCGCTGATCAAGGCCCAATTGCGCAAGCAAGGGATCAATCCGGGCAAGGTGCGCAAGAAATCCG
CGTCGATTTTCAGCTTCGGCAAACGCATCAAGGCCCAGGACATTTCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGTGTGCCGCTGTTGCAGTCGTTCGACATCATCGGCGAAGGCTTCGACAACCCGGCCATGCGCAAACTGGTGGACGA
GGTGAAACAGGAAGTCGCCGCGGGCAACAGCTTCGCCGCATCCCTGCGCAAGAAGCCGCAGTATTTCGACGAGTTGTACT
GCAACCTGGTGGATGCCGGTGAACAGGCCGGCGCACTCGATACCCTGCTGGATCGGGTGGCCACCTATAAAGAAAAGAGC
GAGCAGCTCAAGGCCAAGATCAAGAAGGCCATGACGTATCCGTTGGCCGTGGTGTTCGTCGCGATCATCGTGACGGGGAT
TCTGCTGGTCAAAGTGGTGCCGCAATTCAAGACTGTGTTCTCCGGGTTTGGCGCCGAGTTGCCGTCCTTTACCTTGATGG
TGATCGACTTGTCGGAGTTCATGCAGGAGTGGTGGTGGGTGATGCTCGGCGCGCTGATCGCCGCGATCTTCGGTTTTCGT
CGTGCAATGAAAAAGTCGCAACCCTTTCGCGACCGGATGGACACCTGGCTGCTGAAACTGCCGCTGGTGGGCGCACTGAT
GTACAAGTCTGCCGTGGCGCGTTACGCGCGCACGCTGTCGACCACGTTCGCCGCCGGGGTGCCGTTGGTGGACGCACTCG
AATCAGTGGCCGGCGCCACCGGCAACATCGTGTTCAAACGCGCGGTACTGCGCATCAAGCAGGACGTCTCGACCGGCATG
CAGCTGAATTTCGCCATGCGCACCTCCGGTGTCTTCCCGAACATGGCGATCCAGATGGCCGCCATTGGCGAAGAGTCCGG
CGCACTCGACGACATGCTCGACAAAGTCGCGAGTTTCTACGAGGACGAAGTGGACAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATTATGGTAGTGCTCGGCGTTGTCGTTGGCGGGCTGGTAGTTGCGATGTACCTGCCTATTTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

60.294

100

0.607
  pilC Acinetobacter baumannii D1279779

60.294

100

0.607
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

44.913

99.506

0.447
  pilG Neisseria meningitidis 44/76-A

44.169

99.506

0.44
  pilC Vibrio cholerae strain A1552

42.677

97.778

0.417
  pilC Vibrio campbellii strain DS40M4

40.887

100

0.41
  pilC Thermus thermophilus HB27

37.093

98.519

0.365