Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NK667_RS32325 Genome accession   NZ_CP101125
Coordinates   7283703..7285403 (-) Length   566 a.a.
NCBI ID   WP_054616650.1    Uniprot ID   -
Organism   Pseudomonas nunensis strain In5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7278703..7290403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NK667_RS32290 (NK667_32290) - 7279040..7279666 (-) 627 WP_008154887.1 DUF1780 domain-containing protein -
  NK667_RS32295 (NK667_32295) - 7279801..7280490 (+) 690 WP_054051318.1 energy-coupling factor ABC transporter permease -
  NK667_RS32300 (NK667_32300) - 7280554..7280769 (+) 216 WP_008033212.1 hypothetical protein -
  NK667_RS32305 (NK667_32305) yacG 7280792..7280992 (-) 201 WP_054051315.1 DNA gyrase inhibitor YacG -
  NK667_RS32310 (NK667_32310) coaE 7280989..7281612 (-) 624 WP_054616653.1 dephospho-CoA kinase -
  NK667_RS32315 (NK667_32315) pilD 7281609..7282481 (-) 873 WP_054616652.1 prepilin peptidase Machinery gene
  NK667_RS32320 (NK667_32320) pilC 7282483..7283700 (-) 1218 WP_054616651.1 type II secretion system F family protein Machinery gene
  NK667_RS32325 (NK667_32325) pilB 7283703..7285403 (-) 1701 WP_054616650.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NK667_RS32330 (NK667_32330) - 7285629..7286051 (+) 423 WP_054051305.1 pilin -
  NK667_RS32335 (NK667_32335) - 7286408..7286758 (+) 351 WP_054616649.1 BON domain-containing protein -
  NK667_RS32345 (NK667_32345) - 7287073..7287369 (-) 297 WP_054051301.1 DUF2845 domain-containing protein -
  NK667_RS32350 (NK667_32350) - 7287369..7290311 (-) 2943 WP_054616648.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62734.96 Da        Isoelectric Point: 6.2449

>NTDB_id=708921 NK667_RS32325 WP_054616650.1 7283703..7285403(-) (pilB) [Pseudomonas nunensis strain In5]
MNDIALSGLAKQLVLAELLTEKSAQQAFQQAQRNRISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLHCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDSHATGLEEMADVDLDG
LDIETVDDKNHDSIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKALFMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFSQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSITLIIAQRLARKLCSHCKTPIEIPREALIKEGFPE
ERIGSFTIYEPVGCDHCNHGYKGRIGIYEVVKNTPDLQRLIMAEGNSLEIEIQMRKDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=708921 NK667_RS32325 WP_054616650.1 7283703..7285403(-) (pilB) [Pseudomonas nunensis strain In5]
ATGAATGACATCGCTCTTAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTGCTCACTGAAAAAAGCGCGCAACAGGC
GTTTCAGCAAGCCCAACGCAATCGTATTTCCCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAAGAGCCGGCAAGTGG
CGGAGATTGCCTCGGAACATTTCGGCATGGCCCTGCTGGACCTCCACTGTCTGGACAAGGAAACCCAACCCAAGGGGCTG
GTCAGCGAAAAACTGGTCCGCCAGCACCACGCCCTGCCCCTCTGGCGACGTGGCAATAAACTGTTCGTGGGGATTTCCGA
CCCGACCAATCATCAGGCAATCAACGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCCATCCTGGTAGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTCTTCGACAGCCACGCCACCGGCCTGGAAGAGATGGCCGATGTGGACCTCGATGGC
CTGGACATCGAAACCGTCGACGATAAAAACCACGACTCCATCGCCGGGCAAGACGCCGATGACGCGCCCGTGGTGCGCTT
CGTCCACAAGATGCTGCTCGATGCGATCAAGAGCGGCTCTTCCGACTTGCACTTCGAACCCTACGAAAAAACCTATCGGG
TACGGATGCGCACCGACGGCATCCTGCGCGAAGTTGCCAAACCGCCGATTCAACTGGCTGGGCGAATTGCAGCCCGGCTT
AAGGTTATGGCCAGCCTCGACATTTCCGAAAGACGCAAACCTCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTTCGGGTCAACACCTTGCCGACGCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CCCAGATGGGCATCGACGCCCTCGGTTACGAGCCCGACCAGAAAGCCTTGTTCATGGCCGCCCTCAAGCAGCCACAAGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAAACCGTGTCGCTCTACACCGGGCTGAATATTCTCAATACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAAG
GGCTGGATTTTTCCCAGGCCCTGCGCTCATTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTT
GAAACCGCGGAAATCGCGATCAAGGCCGCCCAGACCGGGCACTTGGTGTTGTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCCTGCATAACATGGGCATCCCGGGGTTCAACATCGCCACCTCGATCACCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGACGCCGATCGAGATTCCCCGTGAAGCGCTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCTTTCACGATCTATGAGCCCGTCGGTTGCGATCACTGCAACCACGGTTACAAAGGGCGAATCGGGAT
TTATGAAGTGGTGAAGAACACGCCAGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCCCTGGAAATCGAGATCCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACTTCCGGGCTGATCAAGGCCATGCAAGGCATTACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.584

99.823

0.585
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

50.545

97.173

0.491
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486