Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J56_RS03620 Genome accession   NZ_AP018768
Coordinates   712813..714207 (+) Length   464 a.a.
NCBI ID   WP_044364868.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-5 isolate 13H40     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707813..719207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J56_RS03595 (CHBNII5_06840) rsmE 707827..708564 (-) 738 WP_044364858.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J56_RS03600 (CHBNII5_06850) lnt 708614..710143 (-) 1530 WP_080334596.1 apolipoprotein N-acyltransferase -
  K6J56_RS03605 (CHBNII5_06860) corC 710166..711065 (-) 900 WP_044364861.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J56_RS03610 (CHBNII5_06870) ampD 711698..712252 (-) 555 WP_044364862.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J56_RS03615 (CHBNII5_06880) pilA 712367..712816 (+) 450 WP_044364865.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J56_RS03620 (CHBNII5_06890) pilB 712813..714207 (+) 1395 WP_044364868.1 GspE/PulE family protein Machinery gene
  K6J56_RS03625 (CHBNII5_06900) pilC 714204..715424 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  K6J56_RS03630 (CHBNII5_06910) pilD 715421..716113 (+) 693 WP_038440789.1 A24 family peptidase Machinery gene
  K6J56_RS03635 (CHBNII5_06920) rho 716167..717429 (-) 1263 WP_005629550.1 transcription termination factor Rho -
  K6J56_RS03640 (CHBNII5_06930) metJ 717677..717994 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J56_RS03645 (CHBNII5_06940) cueR 718008..718394 (-) 387 WP_005663006.1 Cu(I)-responsive transcriptional regulator -
  K6J56_RS03650 (CHBNII5_06950) - 718471..718677 (+) 207 WP_012054944.1 heavy-metal-associated domain-containing protein -
  K6J56_RS03655 (CHBNII5_06960) - 718763..718969 (+) 207 WP_005648955.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52984.41 Da        Isoelectric Point: 5.6445

>NTDB_id=70867 K6J56_RS03620 WP_044364868.1 712813..714207(+) (pilB) [Haemophilus influenzae strain CHBN-II-5 isolate 13H40]
MTSYALLHTQRVIAQNGEIFTISPDLWERNRQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRSLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=70867 K6J56_RS03620 WP_044364868.1 712813..714207(+) (pilB) [Haemophilus influenzae strain CHBN-II-5 isolate 13H40]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATG
GGAACGCAATCGGCAGCAACAATCCTTGCTTTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAGCAACTTACTCCGTGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCTCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCGCTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAACTTGATGGTATTATTCAAAGCCAAATTAATCCGCAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

99.138

100

0.991
  pilB Haemophilus influenzae Rd KW20

96.328

99.784

0.961
  pilB Glaesserella parasuis strain SC1401

57.826

99.138

0.573
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.059

100

0.412
  pilB Acinetobacter baylyi ADP1

38.105

100

0.407
  pilB Acinetobacter baumannii D1279779

38.557

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Neisseria gonorrhoeae MS11

36.555

100

0.375
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment