Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J56_RS03615 Genome accession   NZ_AP018768
Coordinates   712367..712816 (+) Length   149 a.a.
NCBI ID   WP_044364865.1    Uniprot ID   A0AAJ8WBN7
Organism   Haemophilus influenzae strain CHBN-II-5 isolate 13H40     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707367..717816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J56_RS03595 (CHBNII5_06840) rsmE 707827..708564 (-) 738 WP_044364858.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J56_RS03600 (CHBNII5_06850) lnt 708614..710143 (-) 1530 WP_080334596.1 apolipoprotein N-acyltransferase -
  K6J56_RS03605 (CHBNII5_06860) corC 710166..711065 (-) 900 WP_044364861.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J56_RS03610 (CHBNII5_06870) ampD 711698..712252 (-) 555 WP_044364862.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J56_RS03615 (CHBNII5_06880) pilA 712367..712816 (+) 450 WP_044364865.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J56_RS03620 (CHBNII5_06890) pilB 712813..714207 (+) 1395 WP_044364868.1 GspE/PulE family protein Machinery gene
  K6J56_RS03625 (CHBNII5_06900) pilC 714204..715424 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  K6J56_RS03630 (CHBNII5_06910) pilD 715421..716113 (+) 693 WP_038440789.1 A24 family peptidase Machinery gene
  K6J56_RS03635 (CHBNII5_06920) rho 716167..717429 (-) 1263 WP_005629550.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15541.88 Da        Isoelectric Point: 8.4623

>NTDB_id=70866 K6J56_RS03615 WP_044364865.1 712367..712816(+) (pilA) [Haemophilus influenzae strain CHBN-II-5 isolate 13H40]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITTAKGYVASVKTQSGGITVKGNGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=70866 K6J56_RS03615 WP_044364865.1 712367..712816(+) (pilA) [Haemophilus influenzae strain CHBN-II-5 isolate 13H40]
ATGAAATTAACAACACAGCAAACCTTGAAAAAAGGTTTTACATTAATCGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCTTCTGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAACTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAGCCTCAGTGAAAACGCAATCAGGCGGCATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCTGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

95.973

100

0.96
  pilA Haemophilus influenzae Rd KW20

95.302

100

0.953
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila strain ERS1305867

40.136

98.658

0.396
  pilA Vibrio campbellii strain DS40M4

41.135

94.631

0.389
  pilA2 Legionella pneumophila str. Paris

40.288

93.289

0.376

Multiple sequence alignment