Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J55_RS03640 Genome accession   NZ_AP018767
Coordinates   723519..724913 (+) Length   464 a.a.
NCBI ID   WP_110459385.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-4 isolate 13H33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 718519..729913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J55_RS03615 (CHBNII4_06850) rsmE 718534..719271 (-) 738 WP_011271976.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J55_RS03620 (CHBNII4_06860) lnt 719321..720850 (-) 1530 WP_079989936.1 apolipoprotein N-acyltransferase -
  K6J55_RS03625 (CHBNII4_06870) corC 720873..721772 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J55_RS03630 (CHBNII4_06880) ampD 722394..722957 (-) 564 WP_048942277.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J55_RS03635 (CHBNII4_06890) pilA 723073..723522 (+) 450 WP_048942287.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J55_RS03640 (CHBNII4_06900) pilB 723519..724913 (+) 1395 WP_110459385.1 GspE/PulE family protein Machinery gene
  K6J55_RS03645 (CHBNII4_06910) pilC 724910..726130 (+) 1221 WP_048942289.1 type II secretion system F family protein Machinery gene
  K6J55_RS03650 (CHBNII4_06920) pilD 726127..726819 (+) 693 WP_048942290.1 A24 family peptidase Machinery gene
  K6J55_RS03655 (CHBNII4_06930) rho 726873..728135 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J55_RS03660 (CHBNII4_06940) metJ 728383..728700 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J55_RS03665 (CHBNII4_06950) cueR 728714..729100 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J55_RS03670 (CHBNII4_06960) - 729177..729383 (+) 207 WP_005666692.1 heavy-metal-associated domain-containing protein -
  K6J55_RS03675 (CHBNII4_06970) - 729469..729675 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53020.55 Da        Isoelectric Point: 5.8432

>NTDB_id=70833 K6J55_RS03640 WP_110459385.1 723519..724913(+) (pilB) [Haemophilus influenzae strain CHBN-II-4 isolate 13H33]
MTSYALLHTQRVIAQNGEIFMISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQENDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCPKC
GGNLANSCDCHQGYKGRIGVYQFLHWQQNGYQTDFENLRDSGLEKVNQGMTDNKEIERVLGKNL

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=70833 K6J55_RS03640 WP_110459385.1 723519..724913(+) (pilB) [Haemophilus influenzae strain CHBN-II-4 isolate 13H33]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGATATTTATGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTTTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACAGGGAAACCTGTAGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGTCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAAATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGTGCGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCCACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATCATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGCCAAGATCCCGACATCATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAAGGATTGCGCTACGTGC
CGCCCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGACGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAGTATGAAATTGAAAACAGTTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAGCTTTGTCCAAAGTGC
GGTGGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATAAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAAAATGGCTATCAAACGGATTTTGAGAATTTACGAGATAGCGGTCTAGAAAAAGTTAATCAAGGAATGACAGATAATA
AAGAAATTGAACGTGTGTTAGGTAAAAATTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.976

99.784

0.968
  pilB Haemophilus influenzae Rd KW20

95.905

100

0.959
  pilB Glaesserella parasuis strain SC1401

57.768

98.491

0.569
  pilB Vibrio cholerae strain A1552

39.658

100

0.45
  pilB Vibrio campbellii strain DS40M4

40.695

100

0.429
  pilB Acinetobacter baylyi ADP1

39.078

100

0.42
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.351

100

0.418
  pilB Acinetobacter baumannii D1279779

39.709

100

0.412
  pilF Neisseria gonorrhoeae MS11

37.395

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Thermus thermophilus HB27

36.632

100

0.375

Multiple sequence alignment