Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K6J55_RS03650 Genome accession   NZ_AP018767
Coordinates   726127..726819 (+) Length   230 a.a.
NCBI ID   WP_048942290.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-4 isolate 13H33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721127..731819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J55_RS03630 (CHBNII4_06880) ampD 722394..722957 (-) 564 WP_048942277.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J55_RS03635 (CHBNII4_06890) pilA 723073..723522 (+) 450 WP_048942287.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J55_RS03640 (CHBNII4_06900) pilB 723519..724913 (+) 1395 WP_110459385.1 GspE/PulE family protein Machinery gene
  K6J55_RS03645 (CHBNII4_06910) pilC 724910..726130 (+) 1221 WP_048942289.1 type II secretion system F family protein Machinery gene
  K6J55_RS03650 (CHBNII4_06920) pilD 726127..726819 (+) 693 WP_048942290.1 A24 family peptidase Machinery gene
  K6J55_RS03655 (CHBNII4_06930) rho 726873..728135 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J55_RS03660 (CHBNII4_06940) metJ 728383..728700 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J55_RS03665 (CHBNII4_06950) cueR 728714..729100 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J55_RS03670 (CHBNII4_06960) - 729177..729383 (+) 207 WP_005666692.1 heavy-metal-associated domain-containing protein -
  K6J55_RS03675 (CHBNII4_06970) - 729469..729675 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  K6J55_RS03680 (CHBNII4_06980) - 729749..729955 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  K6J55_RS03685 (CHBNII4_06990) - 730029..730235 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26655.61 Da        Isoelectric Point: 8.5626

>NTDB_id=70835 K6J55_RS03650 WP_048942290.1 726127..726819(+) (pilD) [Haemophilus influenzae strain CHBN-II-4 isolate 13H33]
MIYFTMFLLGGILGIALWFYLSGFITRLQQDIYATYVELFPQNFLSFQPSVSSIQQKKCGHILRYFLNVGVEFIFLQIAF
KDSTFAIWLGLTLLILWAISYLDWHYQLISTTLCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=70835 K6J55_RS03650 WP_048942290.1 726127..726819(+) (pilD) [Haemophilus influenzae strain CHBN-II-4 isolate 13H33]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTAGGGATCGCATTGTGGTTCTATCTATCTGGTTTTATTACGCG
TTTACAGCAAGATATTTATGCGACTTACGTTGAATTATTTCCACAAAACTTTCTGTCATTTCAGCCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAATGTTGGGGTAGAATTTATATTTTTACAAATTGCCTTC
AAGGACTCTACTTTCGCTATATGGCTAGGGCTCACACTTCTTATCCTTTGGGCAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACTACACTCTGTTTATGGCTACTTACAATCGGTTTATTTGGAGCAGACAATAATTTTTCATTGCTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae Rd KW20

89.565

100

0.896
  pilD Haemophilus influenzae 86-028NP

89.565

100

0.896

Multiple sequence alignment