Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NMD13_RS04920 Genome accession   NZ_CP101034
Coordinates   1028455..1029672 (-) Length   405 a.a.
NCBI ID   WP_370600744.1    Uniprot ID   -
Organism   Pseudomonas nitroreducens strain OT171     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1023455..1034672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD13_RS04875 (NMD13_04860) - 1023779..1023949 (-) 171 WP_017516731.1 DUF3094 domain-containing protein -
  NMD13_RS04880 (NMD13_04865) - 1024032..1024661 (-) 630 WP_370600738.1 DUF1780 domain-containing protein -
  NMD13_RS04885 (NMD13_04870) - 1024767..1025183 (+) 417 WP_370600739.1 GNAT family N-acetyltransferase -
  NMD13_RS04890 (NMD13_04875) - 1025186..1025653 (+) 468 WP_370600740.1 FAD/FMN-containing dehydrogenase -
  NMD13_RS04895 (NMD13_04880) - 1025650..1026339 (+) 690 WP_370600741.1 energy-coupling factor ABC transporter permease -
  NMD13_RS04900 (NMD13_04885) - 1026447..1026662 (+) 216 WP_331318187.1 hypothetical protein -
  NMD13_RS04905 (NMD13_04890) yacG 1026703..1026906 (-) 204 WP_015475735.1 DNA gyrase inhibitor YacG -
  NMD13_RS04910 (NMD13_04895) coaE 1026903..1027511 (-) 609 WP_370600742.1 dephospho-CoA kinase -
  NMD13_RS04915 (NMD13_04900) pilD 1027577..1028449 (-) 873 WP_370600743.1 A24 family peptidase Machinery gene
  NMD13_RS04920 (NMD13_04905) pilC 1028455..1029672 (-) 1218 WP_370600744.1 type II secretion system F family protein Machinery gene
  NMD13_RS04925 (NMD13_04910) pilB 1029676..1031379 (-) 1704 WP_370600745.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NMD13_RS04930 (NMD13_04915) comP 1031603..1032013 (+) 411 WP_370600746.1 pilin Machinery gene
  NMD13_RS04935 (NMD13_04920) - 1032083..1033867 (+) 1785 WP_370600747.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44309.91 Da        Isoelectric Point: 9.8909

>NTDB_id=708274 NMD13_RS04920 WP_370600744.1 1028455..1029672(-) (pilC) [Pseudomonas nitroreducens strain OT171]
MADKALKTSVFTWEGTDRKGSKIKGELSGTNMALVKAQLRKQGINPTKVRKKGMSLLGQGKKIKPMDIALFTRQMATMMS
SGVPLLQSFDIIGEGFDNPNMRKLIDEIKQEVASGNSLANSLRKKPQYFDNLYCNLVDAGEQSGSLETLLDRVATYKEKT
ESLKAKIRKAMTYPIAVVVVAIIVSSILLIKVVPQFQSVFSSFGAELPAFTRFVISISEALQAWWFIVLILMFITAYALR
TAHTRSEKFRDAVDRLILKLPIVGDILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVNKIKQDVSSGT
QLNFSMRTTGVFPSMAIQMAAIGEESGSLDAMLDKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=708274 NMD13_RS04920 WP_370600744.1 1028455..1029672(-) (pilC) [Pseudomonas nitroreducens strain OT171]
ATGGCGGACAAAGCATTGAAGACCAGTGTCTTTACCTGGGAAGGCACTGATCGGAAGGGCAGCAAGATCAAAGGGGAGCT
TTCCGGTACCAACATGGCGCTGGTCAAGGCCCAGTTGCGCAAACAGGGCATAAACCCCACCAAAGTCCGCAAGAAAGGCA
TGTCGCTGCTGGGCCAGGGCAAGAAGATCAAGCCCATGGACATCGCCCTGTTCACTCGACAGATGGCGACCATGATGAGT
TCGGGCGTTCCGCTGTTGCAGTCGTTCGACATCATCGGTGAAGGTTTTGACAATCCGAACATGCGCAAGCTGATTGACGA
GATTAAACAAGAAGTCGCTTCTGGTAATAGCTTGGCCAACTCCCTGCGCAAGAAGCCGCAGTACTTCGACAACCTATACT
GCAATTTGGTTGACGCAGGCGAACAGTCCGGTTCCCTGGAGACCCTGCTCGACCGGGTGGCGACTTACAAGGAAAAGACC
GAGTCGCTGAAGGCCAAGATCAGGAAGGCGATGACATACCCCATCGCTGTCGTCGTTGTAGCTATCATTGTTTCGTCCAT
TCTGCTGATAAAGGTCGTACCGCAGTTCCAATCGGTGTTCTCTAGTTTTGGAGCAGAGCTTCCAGCATTTACCCGTTTTG
TCATTTCCATTTCGGAAGCGCTGCAGGCCTGGTGGTTCATCGTCCTCATCCTGATGTTCATAACAGCATATGCGCTGCGC
ACCGCCCATACTCGCTCGGAAAAATTCCGGGACGCGGTAGATCGCCTGATACTGAAACTGCCTATTGTCGGCGACATCCT
CTACAAATCAGCCGTCGCCCGTTATGCCCGTACGCTCTCCACGACCTTCGCTGCCGGTGTTCCACTGGTGGAAGCACTGG
ACTCGGTATCCGGTGCGACCGGCAACGTGGTATTCCGCAATGCGGTGAACAAGATCAAGCAGGACGTATCCTCCGGCACC
CAGTTGAACTTCTCCATGCGCACCACCGGCGTGTTCCCGAGCATGGCCATCCAGATGGCCGCCATCGGCGAGGAATCCGG
CTCGCTGGACGCCATGCTCGACAAGGTCGCCAGCTATTACGAGGAAGAGGTCGACAATGCCGTCGATAACCTCACCACAC
TGATGGAGCCAATGATCATGGCCGTCCTGGGCGTCCTGGTTGGCGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAA
CTGGGCAACGTCGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80.741

100

0.807
  pilC Acinetobacter baumannii D1279779

60.494

100

0.605
  pilC Acinetobacter baylyi ADP1

59.951

100

0.602
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria meningitidis 44/76-A

43.781

99.259

0.435
  pilG Neisseria gonorrhoeae MS11

43.532

99.259

0.432
  pilC Vibrio cholerae strain A1552

40.988

100

0.41
  pilC Vibrio campbellii strain DS40M4

39.394

97.778

0.385
  pilC Thermus thermophilus HB27

38.155

99.012

0.378