Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VCA1004_RS01055 Genome accession   NZ_AP018689
Coordinates   227166..228896 (+) Length   576 a.a.
NCBI ID   WP_408646887.1    Uniprot ID   -
Organism   Vibrio aphrogenes strain CA-1004     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 222166..233896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCA1004_RS01035 pilM 224303..225325 (+) 1023 WP_086982011.1 type IV pilus assembly protein PilM Machinery gene
  VCA1004_RS01040 pilN 225315..225929 (+) 615 WP_086982012.1 PilN domain-containing protein Machinery gene
  VCA1004_RS01045 pilO 225929..226513 (+) 585 WP_086982013.1 type 4a pilus biogenesis protein PilO Machinery gene
  VCA1004_RS01050 pilP 226513..227034 (+) 522 WP_086982014.1 pilus assembly protein PilP Machinery gene
  VCA1004_RS01055 pilQ 227166..228896 (+) 1731 WP_408646887.1 type IV pilus secretin PilQ Machinery gene
  VCA1004_RS01060 aroK 229139..229657 (+) 519 WP_086982016.1 shikimate kinase AroK -
  VCA1004_RS01065 aroB 229682..230764 (+) 1083 WP_086982017.1 3-dehydroquinate synthase -
  VCA1004_RS01070 - 230901..232313 (+) 1413 WP_086982018.1 SPOR domain-containing protein -
  VCA1004_RS01075 dam 232410..233231 (+) 822 WP_086982019.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62111.97 Da        Isoelectric Point: 4.4895

>NTDB_id=70388 VCA1004_RS01055 WP_408646887.1 227166..228896(+) (pilQ) [Vibrio aphrogenes strain CA-1004]
MAAPTTTSTVSTTQTSVQPAFSNQFVGLDFRINKQKEGVIVVKLSSSTSAVDIKKNAQGLTIDLIHTRVSDDDLYVIDVK
DFSTAVSTVEVFREGENTRLQAVVSGDFSYDYRLKGNNLEITVTERKATKSAAQSKTVLEGENKKISINFQDIPVRNVLQ
LIAEYNGFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKQIEGNVVLVAPQAELDAQQKQRLEQARMEEELGNLS
SELVQINFAKASDIAALIGGEGEVSMLSSRGNINVDERTNSLLIRDLTTNIEVIKGIIEALDIPVKQVQIEARIVTVSEG
NLQDLGVRWGVSNTNGSFSTGGSIESNLANANRHGQNGRGGLMDGDDLDFAGGDQKVGIDDFLNVNLAATSSNASSIAFQ
VAKLGSDTLLDLELSALQAESKAEIISSPRLITTNKQPAYIEQGTEIPYLEASSSGATSVSFKKAVLSLKVTPQITPDNR
LVLDLSVTQDRPGEVVKTGDGEAVAIDTQRIGTQVLVDNGETVVLGGIYQHSVQTSVDKVPLLGDIPYLGALFRRSYENY
GKSELLIFVTPKVILQ

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=70388 VCA1004_RS01055 WP_408646887.1 227166..228896(+) (pilQ) [Vibrio aphrogenes strain CA-1004]
ATGGCAGCCCCTACGACGACGTCTACGGTTTCCACAACCCAAACTTCGGTACAACCGGCGTTTAGCAATCAATTTGTTGG
GCTCGATTTTCGGATCAATAAACAAAAAGAAGGGGTGATTGTGGTGAAACTCTCCTCTTCAACTTCTGCTGTGGATATTA
AAAAGAATGCCCAAGGCTTAACGATCGATCTGATTCATACGCGTGTCAGTGATGATGACTTATACGTTATTGATGTTAAA
GATTTTTCAACGGCAGTCAGTACCGTTGAGGTCTTTCGAGAGGGGGAGAATACCCGTTTACAAGCGGTGGTGAGCGGTGA
CTTTAGTTATGACTATCGCTTAAAAGGCAACAATCTTGAAATCACCGTCACGGAGAGAAAAGCAACGAAGTCGGCGGCTC
AATCTAAGACGGTGTTAGAAGGGGAAAATAAAAAGATTTCGATTAACTTTCAAGATATTCCGGTGCGAAACGTCCTGCAA
TTAATCGCAGAGTATAACGGTTTTAATCTCGTGGTCTCAGATTCGGTAACGGGTAACCTAACTCTGCGCTTAGATGGCGT
ACCGTGGCAACAAGTGCTGGATATTATTTTGCAAGTAAAAGGACTGGATAAGCAAATTGAAGGTAATGTGGTGTTAGTGG
CTCCGCAAGCTGAACTGGATGCTCAACAAAAACAACGTTTAGAGCAAGCTCGTATGGAAGAAGAGCTTGGCAACTTGAGT
TCAGAGTTGGTGCAAATTAATTTTGCTAAAGCTTCGGATATTGCCGCCTTAATTGGAGGGGAAGGGGAAGTCAGTATGTT
GTCGAGTCGTGGCAATATCAATGTCGATGAACGTACCAACTCGCTGCTTATTCGTGATTTAACCACCAATATTGAAGTGA
TTAAAGGCATTATTGAAGCGTTAGATATTCCGGTAAAGCAGGTGCAAATTGAAGCTCGTATTGTGACTGTGAGTGAGGGG
AATTTGCAAGATCTCGGCGTGCGTTGGGGGGTGAGTAATACTAATGGTTCGTTTTCGACCGGCGGTTCGATTGAAAGCAA
TTTAGCCAATGCCAATCGTCATGGTCAAAATGGTCGTGGTGGTTTAATGGATGGTGATGATTTGGACTTTGCTGGCGGCG
ATCAAAAAGTTGGCATTGATGATTTTTTAAATGTTAATTTAGCCGCAACCAGTTCCAATGCTTCAAGTATTGCCTTTCAA
GTGGCTAAATTAGGCTCTGATACGTTATTGGATTTAGAGTTATCGGCGCTACAAGCTGAATCGAAAGCTGAAATTATTTC
CAGCCCACGCCTGATCACGACCAACAAGCAACCGGCTTATATTGAGCAAGGGACCGAAATTCCTTATTTGGAAGCCTCCT
CCAGTGGTGCTACCTCAGTCAGTTTTAAAAAAGCGGTGCTGAGTTTAAAGGTTACTCCTCAAATTACCCCAGACAATCGC
TTGGTGTTGGATTTGAGTGTGACGCAAGACCGCCCCGGTGAAGTGGTGAAGACTGGAGATGGGGAAGCGGTGGCCATTGA
TACCCAGCGCATCGGAACTCAAGTGCTGGTGGATAATGGAGAAACGGTCGTGTTGGGCGGTATCTATCAACATTCAGTCC
AAACCAGTGTTGATAAAGTGCCTTTGTTAGGTGATATCCCTTATCTTGGGGCGTTATTTCGTCGTAGTTATGAAAATTAT
GGCAAGAGCGAGTTATTGATTTTTGTTACGCCTAAAGTCATTCTACAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(242-307)

Bacterial type II and III secretion system protein (Secretin)

(416-574)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

71.712

96.354

0.691
  pilQ Vibrio cholerae strain A1552

69.894

98.611

0.689
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

69.894

98.611

0.689
  pilQ Legionella pneumophila strain ERS1305867

41.441

96.354

0.399
  pilQ Legionella pneumophila strain Lp02

41.441

96.354

0.399
  pilQ Acinetobacter baumannii D1279779

36.738

100

0.387
  pilQ Acinetobacter baumannii strain A118

36.738

100

0.387
  pilQ Pseudomonas aeruginosa PAK

38.899

97.743

0.38
  comQ Acinetobacter baylyi ADP1

36.622

100

0.38

Multiple sequence alignment