Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VCA1004_RS01045 Genome accession   NZ_AP018689
Coordinates   225929..226513 (+) Length   194 a.a.
NCBI ID   WP_086982013.1    Uniprot ID   -
Organism   Vibrio aphrogenes strain CA-1004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 220929..231513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCA1004_RS01030 - 221469..224039 (-) 2571 WP_086982010.1 penicillin-binding protein 1A -
  VCA1004_RS01035 pilM 224303..225325 (+) 1023 WP_086982011.1 type IV pilus assembly protein PilM Machinery gene
  VCA1004_RS01040 pilN 225315..225929 (+) 615 WP_086982012.1 PilN domain-containing protein Machinery gene
  VCA1004_RS01045 pilO 225929..226513 (+) 585 WP_086982013.1 type 4a pilus biogenesis protein PilO Machinery gene
  VCA1004_RS01050 pilP 226513..227034 (+) 522 WP_086982014.1 pilus assembly protein PilP Machinery gene
  VCA1004_RS01055 pilQ 227166..228896 (+) 1731 WP_408646887.1 type IV pilus secretin PilQ Machinery gene
  VCA1004_RS01060 aroK 229139..229657 (+) 519 WP_086982016.1 shikimate kinase AroK -
  VCA1004_RS01065 aroB 229682..230764 (+) 1083 WP_086982017.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22516.92 Da        Isoelectric Point: 5.7213

>NTDB_id=70386 VCA1004_RS01045 WP_086982013.1 225929..226513(+) (pilO) [Vibrio aphrogenes strain CA-1004]
MVDFRELDLDDMPEWPLLPQLLAIGGLIIVLQIVGTWFYLSPQHDELNQIRQQEVTLKSSIRIKAAKVAALPQLQAQLDE
LNTRYQYLLKQLPVQKELASMLSSVNDMGLKNDLTFTRVDWGEKQVQEFLYRLPLNIELTGAYNNIGQFSEAIAKLPRII
SLHNVEWQRVSQESSTLHFRVRAYTYQFKSQEVQ

Nucleotide


Download         Length: 585 bp        

>NTDB_id=70386 VCA1004_RS01045 WP_086982013.1 225929..226513(+) (pilO) [Vibrio aphrogenes strain CA-1004]
ATGGTCGATTTTCGTGAATTGGATCTTGATGACATGCCAGAATGGCCATTATTACCGCAATTATTAGCGATTGGTGGCCT
CATTATTGTGCTGCAAATTGTGGGTACTTGGTTTTACCTGTCACCGCAACATGATGAGTTAAACCAAATTCGCCAACAAG
AAGTGACACTCAAAAGCTCAATTCGTATCAAGGCGGCCAAAGTGGCTGCTTTACCTCAATTACAAGCTCAATTGGATGAG
CTCAATACCCGTTATCAATATCTACTCAAGCAACTGCCGGTGCAAAAGGAATTAGCCAGCATGTTGTCTTCGGTTAATGA
CATGGGGCTTAAGAATGATTTGACCTTTACGCGAGTGGATTGGGGAGAAAAGCAGGTACAAGAATTCTTGTATCGCTTAC
CGCTGAATATCGAATTAACAGGGGCTTACAACAATATTGGGCAGTTTTCAGAAGCGATTGCTAAGTTGCCTCGTATTATC
AGTTTACACAACGTGGAATGGCAACGAGTCAGCCAAGAGAGCAGCACTTTGCATTTTCGGGTTCGAGCGTACACCTACCA
GTTTAAGTCACAGGAGGTGCAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

67.358

99.485

0.67
  pilO Vibrio campbellii strain DS40M4

63.492

97.423

0.619

Multiple sequence alignment