Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VCA1004_RS01035 Genome accession   NZ_AP018689
Coordinates   224303..225325 (+) Length   340 a.a.
NCBI ID   WP_086982011.1    Uniprot ID   -
Organism   Vibrio aphrogenes strain CA-1004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 219303..230325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCA1004_RS15155 - 220107..220256 (-) 150 WP_164520808.1 hypothetical protein -
  VCA1004_RS01025 oxyR 220479..221369 (+) 891 WP_086982009.1 DNA-binding transcriptional regulator OxyR -
  VCA1004_RS01030 - 221469..224039 (-) 2571 WP_086982010.1 penicillin-binding protein 1A -
  VCA1004_RS01035 pilM 224303..225325 (+) 1023 WP_086982011.1 type IV pilus assembly protein PilM Machinery gene
  VCA1004_RS01040 pilN 225315..225929 (+) 615 WP_086982012.1 PilN domain-containing protein Machinery gene
  VCA1004_RS01045 pilO 225929..226513 (+) 585 WP_086982013.1 type 4a pilus biogenesis protein PilO Machinery gene
  VCA1004_RS01050 pilP 226513..227034 (+) 522 WP_086982014.1 pilus assembly protein PilP Machinery gene
  VCA1004_RS01055 pilQ 227166..228896 (+) 1731 WP_408646887.1 type IV pilus secretin PilQ Machinery gene
  VCA1004_RS01060 aroK 229139..229657 (+) 519 WP_086982016.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37461.12 Da        Isoelectric Point: 6.9163

>NTDB_id=70384 VCA1004_RS01035 WP_086982011.1 224303..225325(+) (pilM) [Vibrio aphrogenes strain CA-1004]
MGKSFVTGIDIGHHSIKAVVVKPVGVQFELVTYAEVPVSGAIFSDNHTVNHQEIVKNLKQLKKQLPMRAKKVVLAVPDSA
VISKLLHIDSELEEREKEFALYQLFAQQSPFPIEELSLDYVKLEPERATVSSTSTYQVYATRKEVIESRVEVIKKSGFTP
WVMDLQAHGLLRVWQMACQEQAEKANGLLVDIGQSQTSICILPAGRPAYYKDFPLGTQQLPSSLTTSQAVSEPSFTLSLE
PPESFIAELAERLTRQLSLYASVNQQHQIDGVWLTGGGATLLGLEESLSTKLNLPVARFNPFARLQGKLTIPERMEPGTF
GVAVGLALKGLQWQGGKHVA

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=70384 VCA1004_RS01035 WP_086982011.1 224303..225325(+) (pilM) [Vibrio aphrogenes strain CA-1004]
ATGGGTAAATCTTTTGTTACGGGTATTGATATCGGTCATCACAGTATAAAAGCTGTGGTTGTTAAGCCCGTTGGAGTGCA
GTTTGAACTGGTGACTTATGCCGAAGTACCAGTAAGCGGCGCTATTTTTTCAGATAACCATACGGTGAATCATCAAGAAA
TTGTCAAAAATCTAAAACAGCTCAAAAAACAGCTTCCGATGAGAGCCAAAAAAGTGGTGTTGGCGGTGCCCGATAGTGCC
GTGATCAGTAAATTGTTGCATATCGATAGTGAACTTGAGGAGCGCGAAAAAGAATTTGCGCTTTATCAGTTGTTTGCTCA
ACAGTCGCCATTTCCCATCGAAGAGTTGAGCTTAGATTATGTCAAGCTTGAACCGGAGCGGGCAACGGTCTCTAGCACGA
GCACTTATCAAGTCTATGCTACCCGCAAAGAGGTAATTGAAAGTCGCGTTGAGGTCATCAAAAAATCGGGCTTTACTCCA
TGGGTGATGGATTTACAAGCGCACGGATTATTACGAGTGTGGCAAATGGCTTGCCAAGAACAAGCCGAAAAAGCCAATGG
CTTATTGGTTGATATCGGTCAAAGCCAAACCTCTATCTGTATTTTGCCGGCAGGGCGTCCCGCTTATTACAAAGATTTTC
CCCTTGGCACGCAGCAGCTGCCTTCCTCGTTAACTACATCACAAGCGGTTTCCGAGCCGAGTTTTACTTTGTCATTGGAA
CCACCTGAGTCCTTTATTGCTGAATTAGCGGAGCGCTTAACTCGGCAATTGAGTTTGTATGCTTCGGTAAACCAGCAACA
TCAGATTGACGGGGTGTGGCTGACCGGTGGAGGAGCGACCTTGCTTGGGCTTGAAGAATCACTCTCTACTAAATTGAATT
TGCCAGTGGCGCGCTTTAACCCTTTTGCTCGCTTACAAGGCAAGTTGACCATTCCTGAGCGTATGGAACCTGGTACTTTT
GGGGTTGCTGTGGGCTTAGCACTCAAAGGGTTGCAGTGGCAAGGAGGAAAACATGTTGCATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

50.146

100

0.506
  pilM Vibrio campbellii strain DS40M4

42.353

100

0.424

Multiple sequence alignment