Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   PCP07_RS20845 Genome accession   NZ_CP115298
Coordinates   4507629..4508093 (-) Length   154 a.a.
NCBI ID   WP_003122079.1    Uniprot ID   P17836
Organism   Pseudomonas aeruginosa strain 37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 4502629..4513093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP07_RS20830 (PCP07_20810) nadC 4505140..4505988 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCP07_RS20840 (PCP07_20820) - 4506169..4507554 (-) 1386 WP_025324686.1 O-antigen ligase family protein -
  PCP07_RS20845 (PCP07_20825) pilA 4507629..4508093 (-) 465 WP_003122079.1 pilin Machinery gene
  PCP07_RS20850 (PCP07_20830) pilB 4508324..4510024 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP07_RS20855 (PCP07_20835) pilC 4510028..4511245 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  PCP07_RS20860 (PCP07_20840) pilD 4511246..4512118 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP07_RS20865 (PCP07_20845) coaE 4512115..4512726 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PCP07_RS20870 (PCP07_20850) yacG 4512723..4512923 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence


Protein


Download         Length: 154 a.a.        Molecular weight: 16277.67 Da        Isoelectric Point: 8.9987

>NTDB_id=702925 PCP07_RS20845 WP_003122079.1 4507629..4508093(-) (pilA) [Pseudomonas aeruginosa strain 37]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSATPKDTQYDIGFTE
STLLDGSGKSQIQVTDNKDGTVELVATLGKSSGSAIKGAVITVSRKNDGVWNCKITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=702925 PCP07_RS20845 WP_003122079.1 4507629..4508093(-) (pilA) [Pseudomonas aeruginosa strain 37]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCGCGACTCCTAAAGATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATAAAGATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTAATCACTGTTTCGCGTAAAAATGACGGAGTCTGGAACTGCA
AAATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  PDB 8V7P

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.737

98.701

0.442

  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

99.351

0.442

  pilA Pseudomonas aeruginosa PAK

45.985

88.961

0.409

  pilA2 Legionella pneumophila strain ERS1305867

39.073

98.052

0.383

  pilA Vibrio parahaemolyticus RIMD 2210633

37.255

99.351

0.37

  pilA2 Legionella pneumophila str. Paris

38

97.403

0.37

  pilA Vibrio cholerae strain A1552

36.774

100

0.37

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.774

100

0.37

  pilA Vibrio cholerae C6706

36.774

100

0.37