Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NI391_RS13625 Genome accession   NZ_CP099951
Coordinates   2980449..2982194 (-) Length   581 a.a.
NCBI ID   WP_315867617.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain Isc25B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2975449..2987194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI391_RS13605 (NI391_13615) - 2976137..2976976 (-) 840 WP_005381301.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  NI391_RS13610 (NI391_13620) - 2977046..2978557 (-) 1512 WP_064354356.1 SPOR domain-containing protein -
  NI391_RS13615 (NI391_13625) aroB 2978610..2979710 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  NI391_RS13620 (NI391_13630) aroK 2979743..2980261 (-) 519 WP_005381319.1 shikimate kinase AroK -
  NI391_RS13625 (NI391_13635) pilQ 2980449..2982194 (-) 1746 WP_315867617.1 type IV pilus secretin PilQ family protein Machinery gene
  NI391_RS13630 (NI391_13640) pilP 2982220..2982735 (-) 516 WP_005381321.1 pilus assembly protein PilP Machinery gene
  NI391_RS13635 (NI391_13645) pilO 2982725..2983318 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI391_RS13640 (NI391_13650) pilN 2983311..2983886 (-) 576 WP_064354358.1 PilN domain-containing protein Machinery gene
  NI391_RS13645 (NI391_13655) pilM 2983870..2984886 (-) 1017 WP_064354359.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63693.70 Da        Isoelectric Point: 4.4593

>NTDB_id=702855 NI391_RS13625 WP_315867617.1 2980449..2982194(-) (pilQ) [Vibrio alginolyticus strain Isc25B]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYMHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAT
SLAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVVQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=702855 NI391_RS13625 WP_315867617.1 2980449..2982194(-) (pilQ) [Vibrio alginolyticus strain Isc25B]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCTGCAGCAGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCGACACCTCGACGACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGCTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGAGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGCTAGGTATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTTGCCACGATCGGTCTCTATAATGGAGAAGGGGGTG
ATGACGGTGGTGACGGTGATGGAGTAGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGTCCGAATGCGACC
AGTCTTGCTTTCCAAGTTGCGAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAGCCTGCCTATATTGAACAAGGTACAGAGATTCCTT
ATCTTGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAAGTGACCCCGCAAATC
ACACCGGATAACCGCTTAGTGTTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTACCTTTGTTGGGCGACCTGCCGCTATTAGGTGCTCTTTTTCGTCGA
AGTTATGAGAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGGTTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.599

100

0.926
  pilQ Vibrio cholerae strain A1552

74.399

100

0.745
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.399

100

0.745
  pilQ Pseudomonas aeruginosa PAK

39.896

99.656

0.398
  pilQ Legionella pneumophila strain Lp02

40.608

96.213

0.391
  pilQ Legionella pneumophila strain ERS1305867

40.608

96.213

0.391
  comQ Acinetobacter baylyi ADP1

37.241

99.828

0.372
  pilQ Acinetobacter baumannii D1279779

35.738

100

0.367
  pilQ Acinetobacter baumannii strain A118

35.738

100

0.367