Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NI391_RS13635 Genome accession   NZ_CP099951
Coordinates   2982725..2983318 (-) Length   197 a.a.
NCBI ID   WP_064354357.1    Uniprot ID   A0AAW7A2J1
Organism   Vibrio alginolyticus strain Isc25B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2977725..2988318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI391_RS13615 (NI391_13625) aroB 2978610..2979710 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  NI391_RS13620 (NI391_13630) aroK 2979743..2980261 (-) 519 WP_005381319.1 shikimate kinase AroK -
  NI391_RS13625 (NI391_13635) pilQ 2980449..2982194 (-) 1746 WP_315867617.1 type IV pilus secretin PilQ family protein Machinery gene
  NI391_RS13630 (NI391_13640) pilP 2982220..2982735 (-) 516 WP_005381321.1 pilus assembly protein PilP Machinery gene
  NI391_RS13635 (NI391_13645) pilO 2982725..2983318 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI391_RS13640 (NI391_13650) pilN 2983311..2983886 (-) 576 WP_064354358.1 PilN domain-containing protein Machinery gene
  NI391_RS13645 (NI391_13655) pilM 2983870..2984886 (-) 1017 WP_064354359.1 type IV pilus biogenesis protein PilM Machinery gene
  NI391_RS13650 (NI391_13660) - 2985031..2987592 (+) 2562 WP_005381325.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23029.56 Da        Isoelectric Point: 5.0885

>NTDB_id=702857 NI391_RS13635 WP_064354357.1 2982725..2983318(-) (pilO) [Vibrio alginolyticus strain Isc25B]
MVDWQDLEIDEMAEWPLLPQIIVIALLIMVIQGLGYWFYLKPKDEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=702857 NI391_RS13635 WP_064354357.1 2982725..2983318(-) (pilO) [Vibrio alginolyticus strain Isc25B]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTATAGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGACGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACCCGTTACGAGTTATTACTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
ACTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

86.842

96.447

0.838
  pilO Vibrio cholerae strain A1552

70.157

96.954

0.68