Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NI391_RS13645 Genome accession   NZ_CP099951
Coordinates   2983870..2984886 (-) Length   338 a.a.
NCBI ID   WP_064354359.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain Isc25B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2978870..2989886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI391_RS13620 (NI391_13630) aroK 2979743..2980261 (-) 519 WP_005381319.1 shikimate kinase AroK -
  NI391_RS13625 (NI391_13635) pilQ 2980449..2982194 (-) 1746 WP_315867617.1 type IV pilus secretin PilQ family protein Machinery gene
  NI391_RS13630 (NI391_13640) pilP 2982220..2982735 (-) 516 WP_005381321.1 pilus assembly protein PilP Machinery gene
  NI391_RS13635 (NI391_13645) pilO 2982725..2983318 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI391_RS13640 (NI391_13650) pilN 2983311..2983886 (-) 576 WP_064354358.1 PilN domain-containing protein Machinery gene
  NI391_RS13645 (NI391_13655) pilM 2983870..2984886 (-) 1017 WP_064354359.1 type IV pilus biogenesis protein PilM Machinery gene
  NI391_RS13650 (NI391_13660) - 2985031..2987592 (+) 2562 WP_005381325.1 penicillin-binding protein 1A -
  NI391_RS13655 (NI391_13665) oxyR 2987646..2988557 (-) 912 WP_064354360.1 DNA-binding transcriptional regulator OxyR -
  NI391_RS13660 (NI391_13670) - 2988727..2989455 (+) 729 WP_005382781.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37553.97 Da        Isoelectric Point: 7.5105

>NTDB_id=702859 NI391_RS13645 WP_064354359.1 2983870..2984886(-) (pilM) [Vibrio alginolyticus strain Isc25B]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGVNCASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEGLSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=702859 NI391_RS13645 WP_064354359.1 2983870..2984886(-) (pilM) [Vibrio alginolyticus strain Isc25B]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGTAAACTGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGGGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTGGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.544

100

0.825
  pilM Vibrio cholerae strain A1552

50.448

99.112

0.5