Detailed information NTG0701169

Bioinformatically predicted

Overview

• Name: comGB/cglB
• Type: Machinery gene
• Locus tag: NEE09_RS01215
• Genome accession: NZ_CP099641
• Coordinates: 238416..239432 (+)
• Length: 338 a.a.
• NCBI ID: WP_078130737.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain ATCC 700671
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 233416..244432

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NEE09_RS01195	nagA	234563..235714 (+)	1152	WP_001134457.1	N-acetylglucosamine-6-phosphate deacetylase	-
NEE09_RS01200	-	235877..236935 (+)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-
NEE09_RS01205	-	237086..237451 (+)	366	WP_000286415.1	DUF1033 family protein	-
NEE09_RS01210	comGA/cglA/cilD	237527..238468 (+)	942	WP_000249536.1	competence type IV pilus ATPase ComGA	Machinery gene
NEE09_RS01215	comGB/cglB	238416..239432 (+)	1017	WP_078130737.1	competence type IV pilus assembly protein ComGB	Machinery gene
NEE09_RS01220	comGC/cglC	239434..239757 (+)	324	WP_000738628.1	comG operon protein ComGC	Machinery gene
NEE09_RS01225	comGD/cglD	239720..240154 (+)	435	WP_033606693.1	competence type IV pilus minor pilin ComGD	Machinery gene
NEE09_RS01230	comGE/cglE	240117..240419 (+)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
NEE09_RS01235	comGF/cglF	240382..240843 (+)	462	WP_000250531.1	competence type IV pilus minor pilin ComGF	Machinery gene
NEE09_RS01240	comGG/cglG	240821..241267 (+)	447	WP_001809944.1	competence type IV pilus minor pilin ComGG	Machinery gene
NEE09_RS01245	-	241370..241962 (+)	593	Protein_226	class I SAM-dependent methyltransferase	-
NEE09_RS01250	comYH	242023..242976 (+)	954	WP_000345126.1	class I SAM-dependent methyltransferase	Machinery gene
NEE09_RS01255	-	243027..244217 (+)	1191	WP_000167765.1	acetate kinase	-
NEE09_RS01260	-	244218..244349 (+)	132	WP_000768904.1	hypothetical protein	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 38523.66 Da
• Isoelectric Point: 9.8240

>NTDB_id=701169 NEE09_RS01215 WP_078130737.1 238416..239432(+) (comGB/cglB) [Streptococcus pneumoniae strain ATCC 700671]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMKSLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide

• Length: 1017 bp

>NTDB_id=701169 NEE09_RS01215 WP_078130737.1 238416..239432(+) (comGB/cglB) [Streptococcus pneumoniae strain ATCC 700671]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGAAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTCCATGGTAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATTTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (24-141)

• Type II secretion system (T2SS), protein F (T2SSF) (209-329)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB/cglB	Streptococcus pneumoniae Rx1	98.817	100	0.988
comGB/cglB	Streptococcus pneumoniae D39	98.817	100	0.988
comGB/cglB	Streptococcus pneumoniae R6	98.817	100	0.988
comGB/cglB	Streptococcus pneumoniae TIGR4	98.817	100	0.988
comGB/cglB	Streptococcus mitis SK321	94.675	100	0.947
comGB/cglB	Streptococcus mitis NCTC 12261	94.083	100	0.941
comYB	Streptococcus gordonii str. Challis substr. CH1	70.833	99.408	0.704
comYB	Streptococcus mutans UA140	58.176	94.083	0.547
comYB	Streptococcus mutans UA159	58.176	94.083	0.547
comGB	Lactococcus lactis subsp. cremoris KW2	50.599	98.817	0.5